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1

The influence of membrane lipid metabolites on lymphocyte potassium channel activity

100%
Teisseyre A., Michalak K., Kuliszkiewicz-Janus M.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr 4
In the present study, the whole-cell patch-clamp technique was applied to elucidate modulatory effects of high-density lipoproteins (HDL), sphingosine (SPH), sphingosine-l-phosphate (SPP), lysophosphatidic acid (LPA) and sphingosyl- phosphorylcholine (SPC) on the activity of Kvl.3 channels in human T lymphocytes (TL). Obtained data provide evidence that application of SPC at micromolar concentrations shifts the channel activation midpoint by about 20 mV towards positive membrane potentials. This effect occurs in a concentration-dependent manner and is saturated at SPC concentrations higher than 10 µM. The shift of channel activation midpoint is accompanied by a pronounced slowing of the activation kinetics. The modulatory effect of SPC is clearly voltage-dependent, being most potent at -20 mV and least potent at +60 mV. The steady-state inactivation curve is also shifted by about 20 mV towards positive membrane potentials. The kinetics of channel inactivation and deactivation (closure) remain unaffected upon SPC treatment. In contrast, application of HDL (250 µg/ml), SPH (50 and 100 µM), SPP (10 µM) and LPA (10 and 36 µM) does not exert any modulatory effect on the channel activity. The effect of SPC on Kvl.3 channel gating resembles the effect exerted by extracellular zinc at the concentration of 10 µM. It is concluded that the effect of SPC is specific and may be due to the presence of a choline residue in SPC molecules. The possible mechanism and the physiological significance of this modulatory effect on Kvl.3 channels are discussed.
2

Lipid changes occuring in the course of hematological cancers

100%
Kuliszkiewicz-Janus M., Malecki R., Mohamed A. S.
Cellular and Molecular Biology Letters
|
2008
|
tom 13
|
nr 3
465-474
The relationship between plasma lipid levels and mortality from cardiovascular diseases has been shown in many studies, but there has been far less investigation into their relationship to non-cardiovascular diseases. The aim of this study was to investigate the lipid profile of individuals with hematological malignancies and its relationship to disease activity. 238 patients were included in the study: 84 with acute leukemia, 62 with non-Hodgkin lymphoma, 35 with Hodgkin’s lymphoma, 32 with multiple myeloma, and 25 with myeloproliferative syndrome. The HDL cholesterol level of the patients differed to that of the individuals in the control group in the active disease period for all the analyzed disorders, but only remained statistically significant in the acute leukemia and non-Hodgkin lymphoma groups during the remission period. Smaller differences were observed for the remaining lipid fractions, except for the triglyceride level, which increased in the active disease period in all the analyzed disorders except non-Hodgkin lymphoma. The most pronounced changes in the lipid fractions occurred in the HDL cholesterol level, and were the most remarkable for acute leukemia.
3

Natural killer (NK) activity in patients with Hodgkin’s disease before, during and after multiple-agent therapy

100%
Frydecka I., Kotlarek-Haus S., Kuliszkiewicz-Janus M., Kuliczkowski K.
Archivum Immunologiae et Therapiae Experimentalis
|
1990
|
tom 38
|
nr 5-6
4

The modulation of lymphocyte potassium channel activity by membrane lipid metabolites

100%
Teisseyre A., Michalak K., Kuliszkiewicz-Janus M.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr Suppl.
5

P MRS analysis of the phospholipid composition of normal human peripheral blood mononuclear cells [PBMC]

88%
Kuliszkiewicz-Janus M., Tuz M. A., Baczynski S., Prajs I., Jazwiec B.
Cellular and Molecular Biology Letters
|
2005
|
tom 10
|
nr 3
6

Spectroscopic properties of selected fluorescence probes used for labelling of lymphocyte or blast cell membranes and plasma high density lipoproteins

88%
Michalak K., Sidorowicz A., Kuliszkiewicz-Janus M., Modrzycka T., Pola A.
Cellular and Molecular Biology Letters
|
1998
|
tom 03
|
nr 2
7

Platelet-activating factor changes in phospholipid extracts from the plasma, peripheral blood mononuclear cells and bone marrow mononuclear cells of patients with acute leukemia - A 31P MRS in vitro study

76%
Kuliszkiewicz-Janus M., Tuz M. A., Kielbinski M., Baczynski S., Jazwiec B., Sladowska H.
Cellular and Molecular Biology Letters
|
2008
|
tom 13
|
nr 1
58-66
The aim of this investigation was to evaluate the changes in PAF concentrations in the plasma, PBMC and BMMC of patients with acute lymphoblastic leukemia (ALL) and acute myeloblastic leukemia (AML). The plasma was from 23 healthy volunteers (HV) and 44 patients with AL (16 ALL, 28 AML). The PBMC were from 15 HV and 55 patients with AL (18 ALL, 37 AML), and the BMMC from 40 patients with AL (11 ALL, 29 AML). Methanol-chloroform phospholipid extraction from 60 × 106 cells (PBMC or BMMC) was performed according to a modified version of Folch’s method. 31P MRS data was obtained on an AMX 300 Bruker spectrometer (7.05 T). The PAF concentration in the plasma of the patients with ALL or AML was lower than that for the healthy volunteers. The PAF concentration in the plasma of the patients with ALL did not differ significantly from that of the patients with AML. In the case of both the PBMC and BMMC, the PAF concentration was significantly diminished in patients with ALL relative to the concentration for those with AML and for the healthy volunteers. No differences were observed in the PAF concentrations for the AML patients and the healthy volunteers.
8

P MRS analysis of the phospholipid composition of the peripheral blood mononuclear cells [PMBC] and bone marrow mononuclear cells [BMMC] of patients with acute leukemia [AL]

76%
Kuliszkiewicz-Janus M., Tuz M. A., Kielbinski M., Jazwiec B., Niedoba J., Baczynski S.
Cellular and Molecular Biology Letters
|
2009
|
tom 14
|
nr 1
35-45
The aim of this study was to evaluate the phospholipid concentration in acute leukemia (AL) blast cells from peripheral blood (PBMC) and bone marrow (BMMC). In vitro 31P Nuclear Magnetic Resonance Spectroscopy (31P MRS) was used. The integral intensities of the resonant peaks and the phospholipid concentrations in PBMC and BMMC were analyzed. Differences in the phospholipid concentrations in cells from myeloblastic or lymphoblastic lines were also evaluated. This investigation was carried out on phospholipid extracts from PBMC and BMMC from 15 healthy volunteers and 77 patients with AL (samples taken at the moment of diagnosis). A significant decrease in sphingomyelin (SM) and phosphtidylserine (PS) was observed in the PBMC of patients with AL relative to the results for the healthy volunteers. For ALL, we found a significant decrease in the concentration of phosphatidylcholine plasmalogen (CPLAS), SM, PI+PE (phosphatidylinositol + phosphatidylethanolamine) and PS in comparison with the results for healthy volunteers and patients with AML. Experiments with BMMC cells revealed a significant decrease in the concentration of CPLAS, SM, PI+PE, and PS in ALL relative to AML. Additionally, a significant decrease in phosphatidylcholine (PC) concentration was observed in ALL compared to AML. If the phospholipid extracts were taken simultaneously from the same patient, there were no significant differences in the integral intensities and phospholipid concentrations between PBMC and BMMC.
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