The experiment was conducted with 38,000 ROSS 308 chickens kept in two buildings of a poultry production farm. The chickens from the experimental group were receiving and addition of a herbal preparation Prisma Jet to a feed mixture. On day 42, the chickens were slaughtered and samples of their intestines were collected for microflora composition analyses and these of meat for qualitative analyses. Determinations were carried out for the counts of lactic acid bacilli Lactobacillus spp., bacteria of the family Enterobacteriaceae sp., mesophilic heterotrophs as well as yeast and filamentous fungi in digesta of the small intestine and blind gut (cecum) of the chickens. In turn, breast and leg muscles were determined for the chemical composition, physicochemical properties and fatty acid profile in the intramuscular and abdominal fat. The study demonstrated that the Prisma Jet preparation evoked a decrease in the total bacterial count in the small intestine and an increase in the count of beneficial intestinal microflora in the cecum. The preparation was also shown to improve meat quality, which was indicated by more favorable physicochemical parameters and fatty acid profile.