Wyniki wyszukiwania

1

Flow karyotyping and chromosome sorting in plants

100%

Dolezel J.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

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1998

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tom 1696

2

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Application of flow cytometry for the study of plant genomes

100%

Dolezel J.

Journal of Applied Genetics

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1997

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tom 38

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nr 3

During the last fifteen years, flow cytometry and sorting greatly contributed to the improvement of our knowledge of plant genome structure and function. This paper reviews the applications of flow cytometry for the analysis of isolated nuclei and chromosomes. Because of its speed, precision and convenience, this method of analysis of the nuclear DNA content finds an enormous number of applications which cover basic research, breeding and production. The results obtained with chromosome analysis and sorting indicate that the technique might greatly simplify the analysis of plant genomes at the molecular level.

3

Karyological characterization of sugar beet gynogenetic lines cultured in vitro

63%

Svirshchevskaya A., Dolezel J.

Journal of Applied Genetics

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2001

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tom 42

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nr 1

4

Cell cycle synchronisation and chromosome isolation in barley [Hordeum vulgare]

51%

Cihalikova J., Lysak M., Dolezel J.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

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1998

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tom 1696

5

Cytogenetic variation in tissue culture-derived Hypericum perforatum L. plants and their seed progenies

51%

Brutovska R., Cellarova E., Dolezel J.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

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1998

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tom 1696

6

Localisation of GAA microsatellites on barley chromosomes by direct cycling-PRINS [C-PRINS]

51%

Kubalakova M., Cihalikova J., Dolezel J.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

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1998

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tom 1696

7

Flow cytometric analysis of nuclear genome size in Musa spp.

51%

Lysak M., Dolezelova M., Dolezel J.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

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1998

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tom 1696

8

Molecular cytogenetics of Musa spp.

51%

Dolezelova M., Valarik M., Dolezel J.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

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1998

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tom 1696

9

Regulation of plant cyclin-dependent kinases and cell cycle machinery by plant hormone analogues

45%

Krystof V., Bogre L., Binarova P., Dolezel J., Strnad M.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 3

10

Multiple, concentration-dependent effects of sucrose, auxins and cytokinins in explant cultures of kale and tobacco

39%

Lustinec J., Cvrckova F., Cizkova J., Dolezel J., Kaminek M., Zarsky V.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 08

We describe complex multiple concentration dependencies for the response of isolated pith tissues to plant biologically active substances. Kale and tobacco stem pith explants were cultured on agar media containing combinations of sucrose, cytokinin [kinetin or benzyladenine (BA)] and auxin [indole-3-acetic acid (IAA) or naphthaleneacetic acid (NAA)]. Absorption of these components by explants and their effects on explant mass, contents of soluble proteins, starch and sugars, and activity of ADP-glucose pyrophosphorylase (AGPase) were studied in relation to their concentration. Up to ten pronounced statistically significant maxima (peaks or waves) were repeatedly detected in the dose–response curves over a concentration range of several logarithmic orders. Slight maxima were observed in the corresponding absorption curves. Pronounced maxima of sucrose absorption were induced by IAA and BA, and those of NAA absorption were induced by sucrose. Both types of multiple maxima (in dose–response and absorption curves) may be due to changes in concentration of intracellular solutes (sugars, auxins and cytokinins), thereby affecting metabolic processes that act as sinks for external solutes and elicit feedback appearance of maxima in absorption curves. Good correspondence between external concentrations at which maxima of different compared curves occur in addition to statistical significance of individual maxima and repeatability of experimental results supports the conclusion that the multiple maxima exhibited are genuine. We consider it possibile that the multiple maxima are associated with endopolyploidy or mixoploidy and/or epigenomic diversity of pith cells that show different sensitivities to biologically active solutes.

11

The bacterial artificial chromosome [BAC] library of the narrow-leaved lupin [Lupinus angustifolius L.]

39%

Kasprzak A., Safar J., Janda J., Dolezel J., Wolko B., Naganowska B.

Cellular and Molecular Biology Letters

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2006

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tom 11

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nr 3

The narrow-leafed lupin possesses valuable traits for environment-friendly agriculture and for the production of unconventional agricultural products. Despite various genetic and environmental studies, the breeding of improved cultivars has been slow due to the limited knowledge of its genomic structure. Further advances in genomics require, among other things, the availability of a genomic DNA library with large inserts. We report here on the construction of the first DNA library cloned in a BAC (bacterial artificial chromosome) vector from diploid Lupinus angustifolius L. cv. Sonet. The high molecular weight DNA used for its preparation was isolated from interphase nuclei that were purified by flow cytometry. The library comprises 55,296 clones and is ordered in 144×384-well microtitre plates. With an average insert size of 100 kb, the library represents six haploid genome equivalents. Thanks to the purification of the nuclei by flow cytometry, contamination with chloroplast DNA and mitochondrial DNA was negligible. The availability of a BAC library opens avenues for the development of a physical contig map and positional gene cloning, as well as for the analysis of the plant’s genome structure and evolution.

Ograniczanie wyników

Flow karyotyping and chromosome sorting in plants

Application of flow cytometry for the study of plant genomes

Karyological characterization of sugar beet gynogenetic lines cultured in vitro

Cell cycle synchronisation and chromosome isolation in barley [Hordeum vulgare]

Cytogenetic variation in tissue culture-derived Hypericum perforatum L. plants and their seed progenies

Localisation of GAA microsatellites on barley chromosomes by direct cycling-PRINS [C-PRINS]

Flow cytometric analysis of nuclear genome size in Musa spp.

Molecular cytogenetics of Musa spp.

Regulation of plant cyclin-dependent kinases and cell cycle machinery by plant hormone analogues

Multiple, concentration-dependent effects of sucrose, auxins and cytokinins in explant cultures of kale and tobacco

The bacterial artificial chromosome [BAC] library of the narrow-leaved lupin [Lupinus angustifolius L.]