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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Optimization of acid assisted extraction process of foxtail millet polysaccharides and its antioxidant activity

100%
Shi Y., Zhu A., Shen L.
International Agrophysics
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2020
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tom 34
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nr 2
2

MiR-139-5p alleviates neural cell apoptosis induced by spinal cord injury through targeting TRAF3

100%
Zhang Z., Shen L., Yan Y.
Acta Biochimica Polonica
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2020
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tom 67
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nr 3
3

Expression of BMP-2 and BMP-4 proteins by type-1 and type-2 astrocytes induced from neural stem cells under different differentiation conditions

76%
Hu J.-G., Zhang Y.-X., Qi Q., Shen L., Xi J., Lu H.-Z.
Acta Neurobiologiae Experimentalis
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2012
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tom 72
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nr 1
Bone morphogenetic proteins (BMPs), a subgroup of the TGF-P superfamily, play critical roles in neural progenitor cell fate determination. Neural stem cells (NSCs) are multipotent progenitor cells that can differentiate into neurons, oligodendrocytes and astrocytes under certain conditions. In our recent report, using an antibody that can recognize both BMP-2 and BMP-4 (BMP-2/4), we showed that BMP-2/4 is only expressed in astrocytes differentiated from NSCs in a medium containing 1% fetal bovine serum (FBS). In this in vitro model, the astrocytic differentiation of NSCs was mainly toward type-2. When NSCs were cultured in a medium containing 10% FBS, most of the cells differentiated into type-1 astrocytes. However, little information is available for BMP-2 and BMP-4 expression in type-1 and type-2 astrocytes induced from NSCs under these different culture conditions. In this study, using two antibodies specific for BMP-2 and BMP-4, respectively, we discriminated the presence of BMP-2 and BMP-4 in NSCs and their derivatives under 1% and 10% FBS culture conditions by RT-PCR, western blot and immunofluorescence staining. We found that BMP-2 and BMP-4 are highly expressed in both type-1 and type-2 astrocytes, and no detectable expression in NSCs, neurons and oligodendrocytes. This suggests that the astrocytes might be one source of BMPs during the differentiation of NSCs. However, in our model, we cannot exclude the possibility that microglia or endothelial cells could also be a source of BMPs.
4

Gene cloning, protein expression and functional analysis of a type 3 metallothionein with bioaccumulation potential from Sonneratia alba

64%
Niu D., Li S., Yi S., Shen L., Huang M., Zhou R., Tan L. C., Breitzig M., Wang F.
Polish Journal of Environmental Studies
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2018
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tom 27
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nr 5
Sonneratia alba (S. alba) is a mangrove species grown in brackish water of tropical and subtropical regions. Due to its unique environment, it has evolved various mechanisms for modulating salt and metal levels. In order to find the genes connected with bioaccumulation of metals, the root transcriptome annotation of Sonneratia alba was analyzed and a new metallothionein (MT) gene was cloned. Sequence analysis found that the new MT gene belongs to type 3 MT, which is mostly expressed in roots. A simple and efficient method was used to express the type 3 MT of S. alba (SaMT3) by transforming the recombinant expression vector pET15b-SaMT3 into Escherichia coli (E. coli) Rosetta-gami and induction with the optimal conditions of 500 μM Isopropyl β-D-1-thiogalactopyranoside (IPTG) at 24ºC for 12 h. OD₆₀₀ of E. coli cells expressing His fused SaMT3 protein after treated with 500 μM Cu²⁺ or 500 μM Pb²⁺ for 12 h can reach 1.01 or 0.98, while OD₆₀₀ of control cells expressing His-tag can reach only 0.81 or 0.75. Both control cells and the cells expressing SaMT3 accumulated metals. Cells expressing SaMT3, however, accumulated more Pb²⁺ and Cu²⁺ (more than two times) than control cells. In vivo, real-time PCR showed that the SaMT3 transcript was induced significantly when stimulated with 250 μM, 500 μM, or 1,000 μM Cu²⁺ or Pb²⁺ for 24 h and 48 h. Taken together, the expression of SaMT3 can increase Cu²⁺ and Pb²⁺ resistance and binding capacity of E. coli.
5

Evolution of the water resources system based on synergetic and entropy theory

64%
Zhu Q., Shen L., Liu P., Zhao Y., Yang Y., Huang D., Wang P., Yang J.
Polish Journal of Environmental Studies
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2015
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tom 24
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nr 6
6
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Relationship of adiponectin, leptin, visfatin and IGF-1 in cow’s venous blood and venous cord blood with calf birth weight

52%
Shen L., Zhu Y., Xiao J., Deng J., Peng G., Zuo Z., Yu S., Ma X., Zhong Z., Ren Z., Zhou Z., Liu H., Zong X., Cao S.
Polish Journal of Veterinary Sciences
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2019
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tom 22
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nr 3
7

Importance of a semen analysis report for determining the relationship between SCSA sperm DNA fragmentation index and assisted reproductive technology pregnancy rate

52%
Cheng H., Han M., Ding J., Wang F., Wang G., Shen L., Wang J., Zheng B., Meng Q., Wang W., Yang S., Li H.
Reproductive Biology
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2020
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tom 20
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nr 4
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Characterization of serum adiponectin and leptin in healthy perinatal dairy cows or cows with ketosis, and their effects on ketosis involved indices

45%
Shen L., Qian B., Xiao J., Zhu Y., Hussain S., Deng J., Peng G., Zuo Z., Zou L., Yu S., Ma X., Zhong Z., Ren Z., Wang Y., Liu H., Zhou Z., Cai D., Hu Y., Zong X., Cao S.
Polish Journal of Veterinary Sciences
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2020
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tom 23
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nr 3
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