Wyniki wyszukiwania

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Antibiotic resistance patterns and resistance genes in enterococci isolated from sheep gastrointestinal tract in Slovakia

100%

Stovcik V., Javorsky P., Pristas P.

Bulletin of the Veterinary Institute in Puławy

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2008

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tom 52

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nr 1

Three hundred Enterococcus isolates from the rumen and faeces of three sheep were analysed for antibiotic resistance. Resistance to tetracycline, kanamycin, and streptomycin was predominant, followed by erythromycin resistance. About 7% of the isolates were resistant to ampicillin and vancomycin only. All tested isolates were susceptible to chloramphenicol. The presence of selected antibiotic resistance determinants was tested by PCR. No tested β-lactamase resistance determinant was detected in ampicillin resistant lactamase positive isolates. Both tet(M) and tet(L) tetracycline resistance determinants were detected. While tet(M) was predominant in both rumen and faecal isolates, clearly a higher frequency of tet(L) determinant was observed in rumen isolates. Among erythromycin resistant isolates, the erm(B) gene was detected only. From this data, it may be concluded that genotypically different enterococcal populations dwell in the upper and lower parts of sheep gastrointestinal tract.

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Factors influencing morphological variability of rumen ciliates from the genus Ophryoscolex

88%

Miltko R., Michalowski T., Pristas P., Javorsky P., Hackstein J. H. P.

Journal of Animal and Feed Sciences. Supplement

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2006

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tom 15

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nr 1

3

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Limited genetic diversity of Aerococcus viridans strains isolated from clinical and subclinical cases of bovine mastitis in Slovakia

76%

Spakova T., Elecko J., Vasil M., Legath J., Pristas P., Javorsky P.

Polish Journal of Veterinary Sciences

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2012

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tom 15

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nr 2

The Aerococcus viridans isolates from bovine mastitis in Slovakia were isolated and characterized by classical microbiological and biochemical, and molecular techniques including IGS-PCR and rep-PCR, ARDRA and 16S rDNA gene sequencing. The substantial variability of antibiotic resistance patterns was observed. The majority of strains were resistant to beta-lactam antibiotics, the resistance to tetracycline was observed in 3 tested strains, resistance to lincomycin was found in 4 strains and practically all tested strains were sensitive to neomycin and ciprofloxacin. While variable at a phenotypic level, no significant genetic variability among A. viridans isolates was detected by molecular DNA based methods. The data obtained suggest that a few A. viridans strains spread among cow's population in Slovak farms.

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Effects of feed supplemented with selenite or Se-yeast on antioxidant enzyme activities in lamb tissues

64%

Sobekova A., Holovska K., Lenartova V., Holovska K.,Jr., Javorsky P., Boldizarova K., Gresakova L., Leng L.

Journal of Animal and Feed Sciences

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2006

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tom 15

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nr 4

569-557

The activities of antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx), glutathione-S-transferase (GST) and glutathione reductase (GR) were examined in liver, kidney cortex and heart tissues of lambs fed diets supplemented with inorganic (sodium selenite) and an organic (Se-yeast) form of selenium. Additional selenium resulted in a significant increase of the Se content in the examined tissues in both supplemented groups. The activities of GSHPx, CAT in the liver as well as of CAT, GST and SOD in the kidney cortex were significantly lower in the Se-yeast supplemented group when compared with both the control and selenite-fed groups. In the heart, the activities of all of the assayed enzymes increased in both supplemented groups. SOD activity was found to be significantly higher in the Se-yeast supplemented group when compared with the selenite group. In addition, two Cu, Zn-SOD isoenzymes of higher band intensity were generated in this group, probably as a result of oxidative stress, which was also manifested by a significant increase of thiobarbituric acid reactive substances (TBARS). The presented results suggest specific regulation of antioxidant enzyme activities in the tissues of lambs depending on the form of selenium intake.

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Antioxidant enzyme activities in liver tissue of chickens fed diets supplemented with various forms and amounts of selenium

64%

Holovska K., Holovska K., Boldizarova K., Cekonova S., Lenartova V., Levkut M., Javorsky P., Leng L.

Journal of Animal and Feed Sciences

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2003

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tom 12

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nr 1

6

Rapid identification of rumen Protozoa by restriction analysis of amplified 18S rRNA gene

51%

Regensbogenova M., Kisidayova S., Michalowski T., Javorsky P., Moon-Van Der Staay S. Y., Moon-Van Der Staay G. W. M., Hackstein J. H. P., McEwan N. R., Jouany J. P., Newbold J. C., Pristas P.

Acta Protozoologica

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2004

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tom 43

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nr 3

Ograniczanie wyników

Antibiotic resistance patterns and resistance genes in enterococci isolated from sheep gastrointestinal tract in Slovakia

Factors influencing morphological variability of rumen ciliates from the genus Ophryoscolex

Limited genetic diversity of Aerococcus viridans strains isolated from clinical and subclinical cases of bovine mastitis in Slovakia

Effects of feed supplemented with selenite or Se-yeast on antioxidant enzyme activities in lamb tissues

Antioxidant enzyme activities in liver tissue of chickens fed diets supplemented with various forms and amounts of selenium

Rapid identification of rumen Protozoa by restriction analysis of amplified 18S rRNA gene