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Isolation and functional expression of a novel lipase gene isolated directly from oil-contaminated soil

100%

Zuo K., Zhang L., Yao H., Wang J.

Acta Biochimica Polonica

2010

tom 57

nr 3

A lipase gene SR1 encoding an extracellular lipase was isolated from oil-contaminated soil and expressed in Escherichia coli. The gene contained a 1845-bp reading frame and encoded a 615-amino-acid lipase protein. The mature part of the lipase was expressed with an N-terminal histidine tag in E. coli BL21, purified and characterized biochemically. The results showed that the purified lipase combines the properties of Pseudomonas chlororaphis and other Serratia lipases characterized so far. Its optimum pH and temperature for hydrolysis activity was pH 5.5-8.0 and 37ºC respectively. The enzyme showed high preference for short chain substrates (556.3 ± 2.8 U/μg for C10 fatty acid oil) and surprisingly it also displayed high activity for long-chain fatty acid. The deduced lipase SR1 protein is probably from Serratia, and is organized as a prepro-protein and belongs to the GXSXG lipase family.

Isolation and characterization of a serine-threonine protein kinase SOS2 gene from Brassica napus

63%

Wang J., Wu W., Zuo K., Fei J., Sun X., Lin J., Li X., Tang K.

Cellular and Molecular Biology Letters

2004

tom 09

nr 3

A full-length cDNA of a new serine/threonine (Ser/Thr) protein kinase gene, designated as BnSOS2 (GenBank Acc. No.AY310413), was cloned from Brassica napus by rapid amplification of cDNA ends (RACE). The fulllength cDNA of BnSOS2 was 1779 bp and contained a 1539-bp open reading frame encoding a protein of 512 amino acids. Homology analysis shows that BnSOS2 strongly resembles other Ser/Thr protein kinase genes, and that its putative protein belongs to a typical Ser/Thr kinase family. Northern blot analysis reveals that BnSOS2 is salt-inducible. Our results indicate that BnSOS2 is a new member of the plant SOS2 gene family, which may play an important role in salt tolerance of plants.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Cellular and Molecular Biology Letters

2 Wang J.

2 Zuo K.

1 Fei J.

1 Li X.

1 Lin J.

1 Sun X.

1 Tang K.

1 Wu W.

1 Yao H.

1 Zhang L.

1 2010

1 2004

Wyniki wyszukiwania

Isolation and functional expression of a novel lipase gene isolated directly from oil-contaminated soil

Isolation and characterization of a serine-threonine protein kinase SOS2 gene from Brassica napus