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Studies on prevalence of infection with Trichomonas tenax identified by molecular techniques - in respect to oral health of patients with various systemic disease requiring immunosuppressive therapy

100%

Dybicz M., Perkowski K., Sedzikowska A., Baltaza W., Chomicz L.

Annals of Parasitology

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2018

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tom 64

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nr 3

Trichomonas tenax, cosmopolitan flagellate inhabiting human oral cavity, is the etiological agent of oral trichomonosis associated with gingival and periodontium deterioration. Purpose of this studies was to investigate the prevalence of infection with Trichomonas tenax identified by molecular techniques amplifying the region of ITS1-5.8S rRNA-ITS2 specific for T. tenax. The study included 498 persons: diabetic, renal transplant, rheumatoid arthritis patients and the control group. Prevalence of T. tenax in oral cavity was 10.2% in control group, 14.1% in diabetics, 12.0% in renal transplant patients and 14.0% in rheumatoid arthritis patients. Comparative assessment of results showed symptoms of gingiva and periodontium deteriorations, at varying intensity in patients with various systemic diseases; higher prevalence of the trichomonad infection was revealed in adults in all groups. Simultaneously, renal transplantation, diabetes, rheumatoid arthritis and related therapy do not affect T. tenax incidences and no increased risk of the infection has been observed in the patients; the permanent medication used due to main disease should be taken into consideration as likely inhibitory factor.

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Examination of oral biofilm microbiota in patients using fixed orthodontic appliances in order to prevent risk factors for health complications

100%

Perkowski K., Balraza W., Conn D. B., Marczynska-Stolarek M., Chomicz L.

Annals of Agricultural and Environmental Medicine

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2019

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tom 26

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nr 2

3

Somatic and typological differentiation of first-year male and female students from the Jozef Pilsudski University of Physical Education in Warsaw

100%

Gryko K., Kopiczko A., Kowalczyk K., Maliszewska I., Perkowski K.

Central European Journal of Sport Sciences and Medicine

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2019

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tom 28

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nr 4

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Molecular identification of Trichomonas tenax in the oral environment of domesticated animals in Poland – potential effects of host diversity for human health

86%

Dybicz M., Perkowski K., Baltaza W., Padzik M., Sedzikowska A., Chomicz L.

Annals of Agricultural and Environmental Medicine

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2018

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tom 25

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nr 3

5

Badania nad wrazliwoscia Trichomonas hominis na czynniki abiotyczne. 1. Wstepna ocena przezywalnosci wiciowcow w wybranych mediach w warunkach in vitro

86%

Chomicz L., Zebrowska J., Zawadzki P., Myjak P., Perkowski K., Rebandel H., Kazimierczuk Z.

Wiadomości Parazytologiczne

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2004

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tom 50

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nr 3

405-409

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Evaluation of selected oral cavity microbiota – risk factors of management complications in patients with masticatory system disorders

86%

Zawadzki P. J., Perkowski K., Starosciak B., Dybicz M., Baltaza W., Pionkowski K., Chomicz L.

Annals of Parasitology

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2016

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tom 62

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nr 1

The retrospective analysis of data on oral cavity clinical status in relation to microbiota species composition is presented. The research regards patients of different age, with and without congenital malformation, pretreatment assessed for occurrence of pathological changes in the masticatory system. Samples of the swabs collected from each patient (from dental plaque, periodontium and dental pockets) were used for identification of oral protozoans in wet slides and stained preparations; additionally, transmission electron microscope examination was performed. The material was used for in vitro cultures to identify bacteria strains. Clinically, intensity of tissue deteriorations was higher in patients with a congenital disease. Alive Trichomonas tenax and Entamoeba gingivalis, species with confirmed pathogenic impact on oral cavity and neighboring structures, were detected with higher prevalence in older patients. Enterococci, Staphylococcus aureus, various Enterobacteriaceae were more frequently detected in patients with somatic and mental retardations; in mouths of those patients, Klebsiella pneumonia and Pseudomonas aeruginosa opportunistic strains occurred. Masticatory system abnormalities favor colonization of oral cavity by exogenous species and dissemination of infections, especially dangerous for patients with congenital diseases. Oral microbiota assessment and preventive measures may be helpful to avoid subsequent peri-surgery complications.

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Evaluation of in vitro effects of selected physical and chemical agents on detected in Poland Acanthamoeba strains - factors of increasing threats for public health

86%

Baltaza W., Padzik M., Szaflik J. P., Perkowski K., Dybicz M., Chomicz L.

Annals of Parasitology

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2019

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tom 65

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nr 1

Acanthamoeba keratitis, the vision-threatening corneal disease reported with increasing frequency in Poland is difficult to treat due to extremely high resistance of the amoeba cysts to chemicals. The agents of possible anti-amoebic activity are still tested. Pathogenic Acanthamoeba samples/isolates acquired from severe cases of keratitis examined by molecular techniques to determine genotypes, compared to one another as well as to the environmental Acanthamoeba castellanii Neff strain were included in the studies. These strains were in vitro examined in terms of their sensitivity/resistance to selected chemicals and tolerance to temperature changes. Samples of the strains cultivated in vitro under bacteria-free conditions were monitored during different growth phases. Higher amoebic population dynamics was observed in both pathogenic Acanthamoeba strains during transfer to 37°C. Agents tested influenced population dynamics in different degree; they showed amoebostatic or amoebicidal effects, however a tendency toward induction of encystment also appeared. Because activation of the dormant cysts can lead to repeated development of amoebae, very important is cysticidal efficacy of chemicals. Further in vitro investigations on various Acanthamoeba strains with different chemicals are still necessary.

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Comparative assessment of selected intraoral microorganisms – potential factors for peri-surgical management complications

86%

Zawadzki P. J., Starosciak B., Perkowski K., Baltaza W., Padzik M., Pionkowski K., Chomicz L.

Annals of Parasitology

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2016

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tom 62

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nr 3

In this research, a comparative analysis of results of investigations involving different human populations, in terms of a relation between the oral cavity health and the species composition of mouth microbiota is reported. The purpose of this analysis was to identify and assess microorganisms that could cause health complications in patients with neoplasm requiring dental problem-related surgical management. The patients with the oral cancer surgically treated and those without neoplasm were assessed for their oral health: status of teeth, gingiva, periodontium, and occurrence of inflammatory processes. From each patient, microorganisms isolated of periodontium, dental plaque, and dental pocket swabs were identified in wet and stained microscopic preparations; standard microbiology in vitro techniques were also applied to determine the fungal and bacterial strains. The comparative analysis of results of direct microscopic examinations and in vitro cultures assessment indicated significant differences in prevalences of fungi, parasitic oral protozoans and bacteriae in particular patient’s groups. Yeast-like fungi belonging to Candida genus, mostly of C. albicans group, were identified in 93.75% patients with the oral cancer, while in 25% of individuals assessed without neoplasm. E. gingivalis amoebae were only found in 12.5% patients with the serious disease; no trichomonads were detected in all patients analyzed. Among bacteria species, potentially pathogenic Enterobacteriaceae were found in the patients with oral cancer. The pronounced shift in the microbiota species composition in the patients who needed prolonged treatment due to oral cavity cancer, compared to other generally healthy persons has been showed in this analysis.

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Składniki mikrobiomu jamy ustnej jako czynniki ryzyka zakażeń lokalnych i uogólnionych u pacjentów bez oraz z wadami wrodzonymi narządu żucia

86%

Perkowski K., Zawadzki P. J., Starosciak B., Dybicz M., Padzik M., Marczynska-Stolarek M., Chomicz L.

Postępy Mikrobiologii

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2016

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tom 55

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nr 1

10

In vivo confocal microscopy and in vitro culture techniques as tools for evaluation of severe Acanthamoeba keratitis incidents

86%

Padzik M., Szaflik J. P., Baltaza W., Perkowski K., Dybicz M., Chomicz L.

Annals of Parasitology

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2017

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tom 63

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nr 4

Amphizoic amoebae belonging to the genus Acanthamoeba are known as etiological agents of sightthreatening Acanthamoeba keratitis. The leading risk factor for the development of this serious human disease is contact lens wearing which popularity increases worldwide, also in Poland. The disease with active epithelial inflammations, corneal ulcers, including loss of the visual acuity is a serious medical problem as an emerging threat for the public health related to improper contact lens hygiene. The treatment of the amoebic keratitis is difficult, often unsuccessful due to delayed proper diagnosis. The clinical picture of the disease, often with severe course is nonspecific, similar to that occurring in viral, fungal or bacterial keratitis, thus clinical symptoms alone are not sufficient to identify the causative agent of the amoebic infection. Early diagnosis is decisive for the suitable therapeutic management and the treatment efficacy. In our studies, several complicated, difficult to treat Acanthamoeba keratitis incidences pertaining Polish patients using contact lenses have been retrospectively analyzed in terms of the usefulness of non-invasive methods of in vivo confocal microscopy and in vitro culture techniques applied for diagnosis. Hyper-reflective double-walled spherical Acanthamoeba cysts, with a more reflective outer wall were detected in the epithelium and anterior layers of the corneal stroma. In vivo confocal microscopy, if available, may be a valuable, sensitive tool for diagnosis in late identified severe infections mainly with strong viability strains, however confoscan may offer limited value at lowintensity amoebic infections. The microscopic visualization of amoebae in slides prepared directly from corneal scraping and laboratory examinations of specimens from in vitro cultivated corneal isolates allow to confirm or verify results of in vivo examinations, furthermore to identify directly the pathogens and to clarify previous misdiagnoses.

Ograniczanie wyników

Studies on prevalence of infection with Trichomonas tenax identified by molecular techniques - in respect to oral health of patients with various systemic disease requiring immunosuppressive therapy

Examination of oral biofilm microbiota in patients using fixed orthodontic appliances in order to prevent risk factors for health complications

Somatic and typological differentiation of first-year male and female students from the Jozef Pilsudski University of Physical Education in Warsaw

Molecular identification of Trichomonas tenax in the oral environment of domesticated animals in Poland – potential effects of host diversity for human health

Badania nad wrazliwoscia Trichomonas hominis na czynniki abiotyczne. 1. Wstepna ocena przezywalnosci wiciowcow w wybranych mediach w warunkach in vitro

Evaluation of selected oral cavity microbiota – risk factors of management complications in patients with masticatory system disorders

Evaluation of in vitro effects of selected physical and chemical agents on detected in Poland Acanthamoeba strains - factors of increasing threats for public health

Comparative assessment of selected intraoral microorganisms – potential factors for peri-surgical management complications

Składniki mikrobiomu jamy ustnej jako czynniki ryzyka zakażeń lokalnych i uogólnionych u pacjentów bez oraz z wadami wrodzonymi narządu żucia

In vivo confocal microscopy and in vitro culture techniques as tools for evaluation of severe Acanthamoeba keratitis incidents