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1

Identification, characterisation and expression analysis of MADS-box genes in sweetpotato wild relative Ipomoea trifida

100%
Zhu P., Dong T., Xu T., Kang H.
Acta Physiologiae Plantarum
|
2020
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tom 42
|
nr 11
2

Progressive collapse test of ship structures in waves

100%
Pei Z., Xu T., Wu W.
Polish Maritime Research
|
2018
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tom 25
|
nr Special Issue S3
The external loads and structural ultimate strength are two important aspects for the safety of ship hull girder. It may collapse in case the structural capacity is less than the external forces in extreme seas. In the present research, progressive collapse test is performed to investigate the collapse mechanism of ship structure in waves. External load with time history and corresponding structural collapse behavior are measured and discussed to demonstrate the interaction of fluid and structures
3

Efficient in vitro plant regeneration of Pinellia ternata [Thunb] Breit

100%
Xu T., Zhang L., Sun X., Tang K.
Acta Biologica Cracoviensia. Series Botanica
|
2005
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tom 47
|
nr 2
An efficient plant regeneration system for Pinellia ternata (Thunb) Breit, an important wild Chinese herb, was established. The investigated factors influencing callus induction and plant regeneration included sterilizing agents, explant type, culture medium, and combinations of plant growth regulators in the medium. Ten min treatment of explants with 0.1% HgCl2 was more suitable for sterilization of tuber explants than NaCIO and H2O2. Tubers as explants were superior to leaves and petioles, and MS medium was better than N6 and White’s media. It was found that 9.1 µM 2,4-dichlorophenoxyacetic acid (2,4-D) plus 4.4 µM 6-benzyladenine (BA) in the medium was the best hormone combination for promoting callus induction. The highest shoot regeneration frequency (99%) was achieved from callus cultured on MS medium supplemented with 9.3 µM kinetin (KN) and 2.3 µM 2,4-D. The rooted shoots were successfully transplanted in soil, with over 95% survival. Establishment of an efficient plant regeneration procedure provides a basis for rapid in vitro propagation of P. ternata, which is in great demand in China, and represents an excellent tool for further work on its genetic manipulation.
4

Pinellia ternata agglutinin produced in Bombyx mori cells exhibits bioactivity

88%
Xu T., Wang B., Wang L., Zhang Y., Lv Z.
Acta Biochimica Polonica
|
2012
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tom 59
|
nr 2
 Pinellia ternata agglutinin (PTA) is highly homologous to many other monocot mannose-binding lectins which reportedly possess antitumor activities. Its production in silkworm cells has great application potential because the baculovirus expression system can produce post-translationally modified proteins at low cost. In the current study, the pta gene was cloned and expressed in silkworm cells, and the expressed protein was analyzed using a hemagglutination assay. A preliminary in vitro study on its anti-proliferative activity was performed. The results show that the recombinant PTA with an apparent molecular mass of 29 kDa can hemagglutinate rabbit erythrocytes and this activity can be inhibited by D-mannan at a low concentration. In addition, the recombinant hemagglutinin exhibited a dose-dependent anti-proliferative activity on hepatoma cells. The results of the current study suggest that PTA and other important bioactive proteins could be produced by silkworm bioreactor for biomedicine research and application.
5

Quantification of potential flood inundation areas in the marsh wetland of Honghe National Natural Reserve, Northeast China

76%
Liu Z., Zhao L., Xu T., Bu F., Liu X., Zhou D.
International Journal of Ecohydrology and Hydrobiology
|
2018
|
tom 18
|
nr 4
6

The toxicity of cerium nitrate to Elodea canadensis: subcellular distribution, chemical forms and physiological effects

76%
Chu W. Y., Cai S. J., Fu Y. Y., Li F. F., Xu T., Qiu H., Xu Q. S.
Acta Physiologiae Plantarum
|
2014
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tom 36
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nr 09
The rare earth elements are increasingly being used as trace supplements in different fields. In this study, subcellular distribution, the chemical forms and toxicity of cerium (Ce) were evaluated for Elodea canadensis. The effect of Ce (5–20 mg L⁻¹) applied for 7 days was assessed by measuring changes in the nutrient elements, photosynthetic pigments, malondialdehyde and antioxidant systems. Ce accumulation was greatest in the cell walls, followed by the organelles and the soluble fraction. Ce levels were higher in cellulose and pectin than in other biomacromolecules. The toxic effects caused by Ce were shown by a reduction in photosynthetic pigments, disruption of nutrient elements, and increases in MDA content. E. canadensis shows Ce-induced oxidative stress by modulating antioxidant enzymes, such as guaiacol peroxidase and catalase. Elevated Ce levels may represent a potential risk for aquatic ecosystems.
7

A light and electron microscopy study of the yak placentome

76%
Xu T., Liu B., Cui Y., He J., Fan J., Yu S.
Folia Morphologica
|
2019
|
tom 78
|
nr 4
Background and Materials and methods: In order to clarify and reveal the morphological characteristics of yak placentomes, placentomes obtained from 151 to 180 days of pregnant yaks were observed using light microscopy and scanning and transmission electron microscopy. Results: The results indicated that sessile, dome-shaped yak placentomes seemed to have a relatively complex villous-crypt architecture pattern. There was a straight maternal plate beneath the placentome. Plentiful uterine glands and a dense cellular layer were present in the endometrium lamina propria close to the maternal plate. Trophoblast giant cells appeared to have similar ultrastructure features to these in other ruminants, including abundant mitochondria, an extensive array of rough endoplasmic reticulum, advanced Golgi complex and many specific secretory granules. Trophoblast giant cells could also secrete neutral and acid glycoconjugates. Furthermore, numerous glycoconjugates were distributed in the connective zones between mononuclear trophoblast cells and crypt epithelial cells as well as in maternal connective tissues. Mononucleate trophoblast cells, which had abundant microvilli that interdigitated with the corresponding microvilli arising from the crypt epithelial cells, had numerous mitochondria and vesicles, but there were no glycoconjugates. Conclusions: The morphological structures of yak placentomes were similar to those of other bovid genera; however, certain differences were observed. These findings might provide morphological evidence for evolutionary relationships between different bovid genera. (Folia Morphol 2019; 78, 4: 818–826)
8

Identification, expression analysis, and function evaluation of 42 tomato DEAD-box RNA helicase genes in growth development and stress response

64%
Cai J., Meng X., Li G., Dong T., Sun J., Xu T., Li Z., Han Y., Zhu M.
Acta Physiologiae Plantarum
|
2018
|
tom 40
|
nr 05
9
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Generation and characterization of a monoclonal antibody against duck Tembusu virus envelope protein

64%
Han K., Zhao D., Liu Y., Liu Q., Huang X., Yang J., Bi K., Xu T., Li Y.
Polish Journal of Veterinary Sciences
|
2016
|
tom 19
|
nr 4
Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused massive economic losses to the duck industry in China. Envelope (E) protein of DTMUV is an important structural protein, which is able to induce protective immune response in target animals and can be used as specific serological diagnosis tool. In this study, a novel monoclonal antibody, designated mAb 3E9, was generated against DTMUV E protein. It is positive in indirect ELISA against both His-E protein and the purified whole viral antigen. Also, this mAb showed positive reaction with DTMUV in Western blot and indirect immunofluorescence assay, and the isotype was IgG1. End-point neutralizing assay performed in BHK-21 cells revealed that the neutralization titer of 3E9 against DTMUV JS804 strain reached 1:50. Furthermore, functional studies revealed that 3E9 blocks infection of DTMUV at a step on viral attachment. The anti-E mAbs produced in the present work may be valuable in developing an antigen-capture ELISA test for antigen detection or a competitive ELISA test for antibody detection or therapeutic medicine for DTMUV in poultry.
10

The role of gibberellins and auxin on the tomato cell layers in pericarp via the expression of ARFs regulated by miRNAs in fruit set

64%
Liu X., Xu T., Dong X., Liu Y., Liu Z., Shi Z., Wang Y., Qi M., Li T.
Acta Physiologiae Plantarum
|
2016
|
tom 38
|
nr 03
11
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Effect of experimental infections of various Tembusu virus strains isolated from geese, ducks and chickens on ducklings

52%
Xu T., Liu X., Liu Q., Han K., Liu Y., Yang J., Huang X., Zhao D., Bi K., Sun W., Li Y.
Polish Journal of Veterinary Sciences
|
2018
|
tom 21
|
nr 2
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