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1

The nucleus of the lateral olfactory tract:comparative morphometric study in common shrew, rabbit and pig

100%
Rowniak M.
Acta Neurobiologiae Experimentalis
|
2001
|
tom 61
|
nr 3
2

The guinea pig (Cavia porcellus) basolateral complex of amygdala: morphometric and Golgi studies

100%
Rowniak M.
Acta Neurobiologiae Experimentalis
|
1999
|
tom 59
|
nr 3
3

The size of neurons in the nuclei of the mammalian amygdala:the comparative morphometric study

51%
Rowniak M., Robak A., Szteyn S.
Acta Neurobiologiae Experimentalis
|
2005
|
tom 65
|
nr 3
4

Expression of calretinin and calbindin in the cingulate cortex of the guinea pig

51%
Hermanowicz-Sobieraj B., Bogus-Nowakowska K., Rowniak M., Robak A.
Acta Neurobiologiae Experimentalis
|
2017
|
tom 77
|
nr Suppl.1
INTRODUCTION: The cingulate cortex (CC), a part of the limbic cortex, is one of the major components of the Papez circuit. Mammalian cerebral cortex contains excitatory pyramidal neurons (70–80%), which use glutamate as neurotransmiter, and interneurons (20–30%), mostly inhibitory, using GABA as principal neurotransmiter. The maintenance a balance between these neurotransmitters is essential for proper functioning of neurons. GABAergic neurons deficit is often related to neurodegenerative disorders. Markers for GABAergic neurons are calcium-binding proteins: calretinin (CR) and calbindin (CB), which may act as calcium sensors as well as both fast and slow calcium buffers. AIM(S): The aim of the study was to describe the distribution of CR and CB and compare expression of both CaBPs at transcriptional and final product levels in the cingulate cortex of the adult guinea pig. METHOD(S): Genes expression of CR and CB was measured on mRNA by quantitative real-time PCR (qPCR) analysis. Total RNA was isolated using Total RNA Mini and then was reverse transcribed to cDNA using Maxima cDNA Synthesis Kit. qPCR was conducted using SYBR® Green JumpStartTMTaqReadyMixTM. To visualize CR and CB immunoreactivity frozen sections were undergone for routine single immunofluorescence labelling, using solution of antibodies raised against CR and CB. RESULTS: The immunohistochemical study indicates the presence of CR and CB in the whole CC. The number of CB-positive perikarya was lower than the CR ones. CR-positive perikarya in comparison to CB-positive, were more numerous in the superficial than in the deep layers of the CC. The qPCR analysis showed that the mRNA expession for CR was higher than for CB. CONCLUSIONS: The CB and CR mRNA expression level revealed by qPCR correlate with their protein abundance level revealed by immunohistochemistry. Calretinin expression was higher than calbindin at both levels. FINANCIAL SUPPORT: Co‑financing the scientific studies for young scientists or PhD students at the Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn (ID:12.620.026-300).
5

Developmental changes of calbindin immunoreactivity in the preoptic area of the male guinea pig (Cavia porcellus)

51%
Bogus-Nowakowska K., Hermanowicz-Sobieraj B., Rowniak M., Robak A.
Acta Neurobiologiae Experimentalis
|
2017
|
tom 77
|
nr Suppl.1
INTRODUCTION: Calbindin (CB) is one of the members of the EF-hand family of calcium-binding proteins which are involved in controlling intracellular calcium ion homeostasis. It may act as Ca2+ “fast” buffers and also as Ca2+ sensors. Intracellular calcium ions play an important role in immature and mature neurons. During early stages of development, calcium ions are involved e.g. in neuronal differentiation and plasticity, migration of neurons, or extension of neuronal processes. The preoptic area (POA) is a key structure which takes part in many autonomic functions (for example thermoregulation, thirst or hunger) as well as in reproduction and maternal behaviour, especially for pup retrieval as well as the onset of parental behaviour in females and males. AIM(S): The aim of the study was to examine the distribution of CB expression during the development of the preoptic area in the guinea pig by means of immunohistochemistry. METHOD(S): Brains from fetal stages (E50, E60), newborns (P0) and postnatal stages (P10, P20, P40, P100) were fixed in 4% paraformaldehyde in phosphate buffer and then cryoprotected. Frozen sections were processed for two immunohistochemical methods: an immunoenzymatic and immunofluorescence. RESULTS: Calbindin was highly expressed in the preoptic area of the male guinea pig, especially in the periventricular region. CB- immunoreactive (-ir) perikarya, fibers and punctate structures were observed at each examined stages. CB-ir perikarya were the most numerous at E50 and the least numerous at P100. The CB-ir neurons had oval, rounded or polygonal perikarya and some of them had processes of various length which emerged from perikarya. CB-ir fibers differed according to lengths. CONCLUSIONS: The highest expression of CB in the preoptic area at E50 coincides well with major developmental events (i.e. eyes opening) which in the guinea pig occur just before E50 stage.
6

The neuronal structure of the red nucleus in newborn guinea pigs

51%
Robak A., Szteyn S., Bogus-Nowakowska K., Rowniak M.
Folia Morphologica
|
2003
|
tom 62
|
nr 1
The preparations, stained according to the Nissl and Klüver-Barrera methods, were used to describe the topography and morphology of the red nucleus (RN) as well as the structure of the rubral perikarya in newborn (P0) guinea pigs. The Golgi impregnated preparations were used to distinguish types of neurons. RN is a uniform cell group and has the length from 740 to 860 µm. The Nissl stained perikarya were classified into three categories: big, medium-sized and small (A, B, C, respectively). The big perikarya, which contain a lot of tigroidal substance, were mainly observed at the caudal and ventral portions of RN. The small perikarya often have multiple nucleoli. The impregnated neurons were classified into 5 types: 1 — large, aspiny, rich-arborised multipolar cells, 2 — large and medium sized, spiny, rich-arborised fusiform or pear-shaped cells, 3 — medium-sized, spiny, rich-arborised rounded cells, 4 — medium-sized, spiny, richarborised bipolar cells, 5 — small and single medium-sized cells. The 5th type constitutes a heterogeneous population and also has neurons in different developmental stages. Intraspecies variations concerning both the length of RN and a number of the triangular perikarya of the red nucleus were observed in the examined guinea pigs.
7

Structure and topography of the nuclei of mamillary region in the Bison bonasus

51%
Robak A., Szteyn S., Rowniak M.
Folia Morphologica
|
1995
|
tom 54
|
nr 2
8

The types of neurons of the somatic oculomotor nucleus in the European bison. Nissl and Golgi studies

51%
Szteyn S., Robak A., Rowniak M.
Folia Morphologica
|
1997
|
tom 56
|
nr 4
9

The neuronal structure of globus pallidus in Bison bonasus: Nissl and Golgi study

51%
Rowniak M., Szteyn S., Robak A.
Folia Morphologica
|
1995
|
tom 54
|
nr 3
10

The neuronal structure of paramamillary nuclei in Bison bonasus: Nissl and Golgi pictures

51%
Robak A., Szteyn S., Rowniak M.
Folia Morphologica
|
1998
|
tom 57
|
nr 2
11

The types of neurones in the neostriatum of the guinea pig [Cavia porcellus]: Golgi and Kluver-Barrera studies

51%
Szteyn S., Robak A., Rowniak M.
Folia Morphologica
|
2000
|
tom 59
|
nr 1
The Golgi technique stain was used to reveal the cellular structure of the neostriatum (nucleus caudatus and putamen) in the guinea pig. The computerised reconstructions were made from Golgi impregnated neurones. On the basis of various criteria, 4 types of neurones were distinguished in the guinea pig neostriatum: 1. The rounded neurones (most numerous) with 5–8 thin dendritic trunks; 2. The triangular nerve cells with 3 thick dendritic trunks; 3. Two types of multipolar neurones differing in dendritic arborisation pattern with 4–6 and 7–9 primary dendrites, respectively. 4. The pear-shaped cells, which divide into two distinctly different subpopulations.
12

A morphometric study of the amygdala in the common shrew

51%
Rowniak M., Szteyn S., Robak A.
Folia Morphologica
|
2004
|
tom 63
|
nr 4
The characteristic features of the common shrew amygdala (CA), as shown by volumetric comparisons of the individual nuclei, are the poor development of the lateral (LA) and basomedial (BM) nuclei as well as the particularly strong formation of the basolateral (BL) and lateral olfactory tract (NLOT) nuclei. The central (CE), cortical (CO) and medial (ME) nuclei are also well organised in this species. All these features are even more distinctly visible when the total number of neurons in the nuclei referred to are compared. A comparison of the densities of neurons in the individual nuclei with the mean numerical density of cells in the CA indicates that there are the 3 different regions within the common shrew’s CA. The densities of neurons in the LA, BL, and BM are significantly lower than the mean density of cells in the CA (p < 0.05). In the CE this value does not differ from the mean (p > 0.05). In the CO, ME and NLOT the density values are significantly higher than the mean (p < 0.05). Furthermore, a similar division of the shrew’s CA can, to some extent, be performed using the size parameters of the amygdaloid neurons as a marker. Interestingly, the large neurons populate less densely organised CA areas like the LA, BL and BM, whereas the small cells populate the ME and NLOT, where the neurons are densely arranged. The CE and CO occupy intermediate positions, with the neurons similar in size to the mean for the shrew’s CA.
13

The types of neurons in the claustrum of Bison bonasus: Nissl and Golgi study

51%
Rowniak M., Szteyn S., Robak A., Klawon M.
Folia Morphologica
|
1994
|
tom 53
|
nr 4
14

Neurons of the substantia nigra in the European bison

51%
Szteyn S., Robak A., Rowniak M.
Folia Morphologica
|
1995
|
tom 54
|
nr 4
15

Types of neurons in the nucleus ruber of the European bison. Kluver-Barrea and Golgi studies

51%
Szteyn S., Rowniak M., Robak A.
Folia Morphologica
|
1998
|
tom 57
|
nr 4
16

Location and structure of the nuclei of epithalamus in hedgehog

51%
Robak A., Szteyn S., Rowniak M.
Folia Morphologica
|
1997
|
tom 56
|
nr 2
17

The cytoarchitectonic and neuronal structure of the red nucleus in guinea pig: Nissl and Golgi studies

45%
Robak A., Szteyn S., Bogus-Nowakowska K., Doboszynska T., Rowniak M.
Folia Morphologica
|
2000
|
tom 59
|
nr 4
The present studies were carried out on the brains of adult guinea pigs, DunkinHartley strain. On the basis of preparations, they were stained according to the Nissl and the Klüver-Barrera method’s; a short description of the cytoarchitectonics and the characteristics of the rubral cells were written. The red nucleus (RN) of the guinea pig is 1.2 mm in length. Three cellular parts in RN, and three classes (A, B, C) of the rubral cells were distinguished. Taking into consideration the predominant cell size, RN was divided into magnocellular part (RNm), parvocellular part (RNp) and intermediate part (RNi). On the basis of Golgi impregnated preparations four neuronal types (I, II, III, IV) were distinguished. To sum up, in the guinea pig were observed: the large, mainly multipolar (type I) and bipolar (type II) spiny being coarse (class A) in Nissl material; the medium-sized, triangular, aspiny (type III) corresponding to the fine cells (class B); and the small, both spiny and aspiny neurons (type IV), which are the fine or achromatic cells (classes B or C) in Nissl stained slices. The highest degree of dendritic branching was observed in type I, whereas the lowest in cells of types III and IV.
18

The types of neurons in the neostriatum of Bison bonasus Nissl and Golgi study

45%
Rowniak M., Szteyn S., Robak A., Klawon M., Dusza M.
Folia Morphologica
|
1994
|
tom 53
|
nr 3
19

A morphometric comparative study of the lateral geniculate body in selected placental mammals: the common shrew, the bank vole, the rabbit, and the fox

39%
Najdzion J., Wasilewska B., Bogus-Nowakowska K., Rowniak M., Szteyn S., Robak A.
Folia Morphologica
|
2009
|
tom 68
|
nr 2
The lateral geniculate body (LGN) was morphometrically examined and compared in representatives of four mammalian orders (Insectivora, Rodentia, Lagomorpha, and Carnivora). In each studied species, the lateral geniculate body was divided into two distinct parts: the dorsal nucleus (LGNd) and the ventral nucleus (LGNv). The lateral geniculate body of the common shrew and the bank vole are very similar in appearance and nuclear pattern. The dorsal and ventral nuclei of these two species also have the most similar statistical characteristics. The lateral geniculate body of the fox has the most complicated morphology and multilayered structure. A significant disproportion was observed between the sizes of both geniculate nuclei in the fox, where the dorsal nucleus definitely surpassed the ventral nucleus in terms of volume. With the exception of the fox, the neuronal density of the LGN nuclei was negatively correlated with the volumes of the LGN. The mean neuronal size of the LGNd and LGNv, which was the resultant of the length, width, area, and circumference of the soma, grew correlatively to the volumes of these nuclei. In all examined species, somas of the LGNd neurons are distinctly larger and have more similar shapes than the LGNv perikarya. In addition, the numerical density of neurons in the ventral nucleus is significantly higher than in the dorsal nucleus. All these morphometric parameters clearly differentiate the LGNd from the LGNv (Folia Morphol 2009; 68, 2: 70–78).
20

Calcium - binding proteins immunoreactivity in Cavia porcellus (Rodentia) hippocampus 30 days after postconception

39%
Robak A., Hermanowicz-Sobieraj B., Bogus-Nowakowska K., Rowniak M., Wasilewska B., Kolenkiewicz M.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2018
|
tom 60
|
nr 1
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