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Axonal outgrowth stimulation after alginate/mesenchymal stem cell therapy in injured rat spinal cord

100%

Blasko J., Szekiova E., Slovinska L., Kafka J., Cizkova D.

Acta Neurobiologiae Experimentalis

2017

tom 77

nr 4

Despite strong efforts in the field, spinal cord trauma still belongs among the untreatable neurological conditions at present. Given the complexity of the nervous system, an effective therapy leading to complete recovery has still not been found. One of the potential tools for supporting tissue regeneration may be found in mesenchymal stem cells, which possess anti‑inflammatory and trophic factor‑producing properties. In the context of transplantations, application of degradable biomaterials which could form a supportive environment and scaffold to bridge the lesion area represents another attractive strategy. In the present study, through a combination of these two approaches we applied both alginate hydrogel biomaterial alone or allogenic transplants of MSCs isolated from bone marrow seeded in alginate biomaterial into injured rat spinal cord at three weeks after spinal cord compression performed at Th8‑9 level. Following three‑week survival, using immunohistochemistry we studied axonal growth (GAP‑43 expression) and both microglia (Iba‑1) and astrocyte (GFAP) reactions at the lesion site and in the segments below and above the lesion. To detect functional improvement, during whole survival period we performed behavioral analyses of locomotor abilities using a classical open field test (BBB score) and a Catwalk automated gait analyzing device (Noldus). We found that despite the absence of locomotor improvement, application of both alginate and MSCs caused significant increase in the number of GAP‑43 positive axons.

Hypothermia in the course of acute traumatic spinal cord injury

86%

Kafka J., Lukacova N., Sulla I., Maloveska M., Vikartovska Z., Cizkova D.

Acta Neurobiologiae Experimentalis

2020

tom 80

nr 2

In this review we briefly discuss animal experiments involving acute traumatic spinal cord injury (SCI) and the need for larger animals in testing experimental therapies. This literature overview, including the discussion of our own results from animal models, examines the use of hypothermia as a treatment method for SCI. Finally, we report the results of hypothermia application in clinical trials. Minipigs have been proposed as a potentially preferable model to rodents (typically rats) for predicting outcomes in human SCI due to their closer anatomical similarity to humans. In various animal studies, hypothermic treatment applied in the acute phase after SCI has resulted in neuroprotective effects, most likely due to inhibition of blood flow and oxygen consumption and reduction of overall metabolic activity and inflammation, resulting in improved nerve tissue sparing. Small‑scale human clinical trials have been carried out, involving general (whole‑body, systemic) or local hypothermia (close to the SCI site), with encouraging results. Nevertheless, further multi‑center, randomized, double‑blind studies with much larger patient numbers are necessary so that protocols can be standardized in order for hypothermia treatment to be reliably applied in clinical practice.

Ograniczanie wyników

2 Acta Neurobiologiae Experimentalis

2 Cizkova D.

2 Kafka J.

1 Blasko J.

1 Lukacova N.

1 Maloveska M.

1 Slovinska L.

1 Sulla I.

1 Szekiova E.

1 Vikartovska Z.

1 2020

1 2017

Wyniki wyszukiwania

Axonal outgrowth stimulation after alginate/mesenchymal stem cell therapy in injured rat spinal cord

Hypothermia in the course of acute traumatic spinal cord injury