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P MRS analysis of the phospholipid composition of normal human peripheral blood mononuclear cells [PBMC]

100%

Kuliszkiewicz-Janus M., Tuz M. A., Baczynski S., Prajs I., Jazwiec B.

Cellular and Molecular Biology Letters

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2005

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tom 10

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nr 3

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Platelet-activating factor changes in phospholipid extracts from the plasma, peripheral blood mononuclear cells and bone marrow mononuclear cells of patients with acute leukemia - A 31P MRS in vitro study

86%

Kuliszkiewicz-Janus M., Tuz M. A., Kielbinski M., Baczynski S., Jazwiec B., Sladowska H.

Cellular and Molecular Biology Letters

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2008

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tom 13

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nr 1

58-66

The aim of this investigation was to evaluate the changes in PAF concentrations in the plasma, PBMC and BMMC of patients with acute lymphoblastic leukemia (ALL) and acute myeloblastic leukemia (AML). The plasma was from 23 healthy volunteers (HV) and 44 patients with AL (16 ALL, 28 AML). The PBMC were from 15 HV and 55 patients with AL (18 ALL, 37 AML), and the BMMC from 40 patients with AL (11 ALL, 29 AML). Methanol-chloroform phospholipid extraction from 60 × 106 cells (PBMC or BMMC) was performed according to a modified version of Folch’s method. 31P MRS data was obtained on an AMX 300 Bruker spectrometer (7.05 T). The PAF concentration in the plasma of the patients with ALL or AML was lower than that for the healthy volunteers. The PAF concentration in the plasma of the patients with ALL did not differ significantly from that of the patients with AML. In the case of both the PBMC and BMMC, the PAF concentration was significantly diminished in patients with ALL relative to the concentration for those with AML and for the healthy volunteers. No differences were observed in the PAF concentrations for the AML patients and the healthy volunteers.

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P MRS analysis of the phospholipid composition of the peripheral blood mononuclear cells [PMBC] and bone marrow mononuclear cells [BMMC] of patients with acute leukemia [AL]

86%

Kuliszkiewicz-Janus M., Tuz M. A., Kielbinski M., Jazwiec B., Niedoba J., Baczynski S.

Cellular and Molecular Biology Letters

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2009

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tom 14

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nr 1

35-45

The aim of this study was to evaluate the phospholipid concentration in acute leukemia (AL) blast cells from peripheral blood (PBMC) and bone marrow (BMMC). In vitro 31P Nuclear Magnetic Resonance Spectroscopy (31P MRS) was used. The integral intensities of the resonant peaks and the phospholipid concentrations in PBMC and BMMC were analyzed. Differences in the phospholipid concentrations in cells from myeloblastic or lymphoblastic lines were also evaluated. This investigation was carried out on phospholipid extracts from PBMC and BMMC from 15 healthy volunteers and 77 patients with AL (samples taken at the moment of diagnosis). A significant decrease in sphingomyelin (SM) and phosphtidylserine (PS) was observed in the PBMC of patients with AL relative to the results for the healthy volunteers. For ALL, we found a significant decrease in the concentration of phosphatidylcholine plasmalogen (CPLAS), SM, PI+PE (phosphatidylinositol + phosphatidylethanolamine) and PS in comparison with the results for healthy volunteers and patients with AML. Experiments with BMMC cells revealed a significant decrease in the concentration of CPLAS, SM, PI+PE, and PS in ALL relative to AML. Additionally, a significant decrease in phosphatidylcholine (PC) concentration was observed in ALL compared to AML. If the phospholipid extracts were taken simultaneously from the same patient, there were no significant differences in the integral intensities and phospholipid concentrations between PBMC and BMMC.

Ograniczanie wyników

3 Cellular and Molecular Biology Letters

3 Baczynski S.

3 Jazwiec B.

3 Kuliszkiewicz-Janus M.

3 Tuz M. A.

2 Kielbinski M.

1 Niedoba J.

1 Prajs I.

1 Sladowska H.

1 2009

1 2008

1 2005

P MRS analysis of the phospholipid composition of normal human peripheral blood mononuclear cells [PBMC]

Platelet-activating factor changes in phospholipid extracts from the plasma, peripheral blood mononuclear cells and bone marrow mononuclear cells of patients with acute leukemia - A 31P MRS in vitro study

P MRS analysis of the phospholipid composition of the peripheral blood mononuclear cells [PMBC] and bone marrow mononuclear cells [BMMC] of patients with acute leukemia [AL]