Wyniki wyszukiwania

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Application of mass spectrometry [MS] in the structural analysis od selected polypeptides of boar seminal plasma obtained after two-dimensional electrophoresis [2-D PAGE]

100%

Kordan W., Malinowska A., Mogielnicka M., Lecewicz M.

Animal Science Papers and Reports

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2009

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tom 27

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nr 1

41-50

Two-dimensional electrophoresis (2-D PAGE) led to identification in the polypeptide maps of boar seminal plasma of four conserved polypeptides with identical molecular weight of 24 kDA, and different ranges of isoelectric point (pI): (1) 7.4-7.7, (2) 8.1-8.4, (3) 8.5-8.8 and (4) 9.2-9.4. In the current study the molecular structures of these polypeptides were analysed, for the first time, by mass spectrometry (LC – MS/MS). Computerized mass spectrometry analysis of the peptides obtained after trypsin-digestion of the polypeptides demonstrated their similarity to the family of spermadhesins (crystal structure of two members of the spermadhesin family), especially to epididymal spermadhesin AWN-1. In addition, homology was found of peptides 3 and 4 with a lysozyme C precursor (1,4-beta-N-acetylmuramidase C). The results presented might indicate the participation of the analysed polypeptides in the processes accompanying fertilization.

2

Mapowanie bialek plazmy nasienia knura przy wykorzystaniu metody elektroforezy dwukierunkowej w zelu poliakryloamidowym

88%

Kordan W., Strzezek J., Soliwoda D., Lecewicz M., Mogielnicka M.

Medycyna Weterynaryjna

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2008

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tom 64

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nr 02

223-226

An attempt was made to use a modified 2-D PAGE technique to analyze seminal plasma proteins and their polymorphisms in relation to boar age and season. The 2-D PAGE analysis of seminal plasma proteins was performed using a buffer pH gradient of 3 to 10. Modifications to the 2-D PAGE procedure included substituting mercaptoethanol (ME) with dithiothreitol (DTT) and the use of a specific reagent assay (Plus One 2-D Clean-Up Kit, Amersham Biosciences), which markedly improved the resolution of the electrophoregrams. Polymorphisms by polypeptide mapping of boar seminal plasma were dependent on the animal age and season. Furthermore, the amount of polypeptides detected in the seminal plasma was significantly lower in 12 month-old boars compared with 3 year-olds. Additionally, the seminal plasma polypeptides were markedly lower in the summer than in the autumn. The results of the study showed that mapping seminal plasma proteins may be used as a marker for the secretor activity of boar accessory sex glands, and as a selection criterion for male reproduction.

3

Proteomics of boar seminal plasma - current studies and possibility of their application in biotechnology of animal reproduction

76%

Strzezek J., Wysocki P., Kordan W., Kuklinska M., Mogielnicka M., Soliwoda D., Fraser L.

Reproductive Biology

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2005

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tom 05

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nr 3

Ograniczanie wyników

1 Animal Science Papers and Reports

1 Medycyna Weterynaryjna

1 Reproductive Biology

3 Kordan W.

3 Mogielnicka M.

2 Lecewicz M.

2 Soliwoda D.

2 Strzezek J.

1 Fraser L.

1 Kuklinska M.

1 Malinowska A.

1 Wysocki P.

1 2009

1 2008

1 2005

Application of mass spectrometry [MS] in the structural analysis od selected polypeptides of boar seminal plasma obtained after two-dimensional electrophoresis [2-D PAGE]

Mapowanie bialek plazmy nasienia knura przy wykorzystaniu metody elektroforezy dwukierunkowej w zelu poliakryloamidowym

Proteomics of boar seminal plasma - current studies and possibility of their application in biotechnology of animal reproduction