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Identification of salt-tolerant barley varieties by a consolidated physiological and molecular approach

100%

Kamboj A., Ziemann M., Bhave M.

Acta Physiologiae Plantarum

2015

tom 37

nr 01

Soil salinity is a significant international environmental problem and severely affects the yield of cereal crops due to numerous effects on plant–water relations, ion homeostasis and salt toxicity. This work assessed the salt tolerance levels of 16 barley varieties by key physiological and molecular tests. Seedlings were exposed to acute salinity stress and their physiological responses including relative water content, levels of the stress hormone abscisic acid (ABA) and Na?/K? ratio were compared. A significant variation was noted amongst the varieties, with Calmariout, Hindmarsh and Mundah identified as tolerant varieties and Franklin as the most sensitive variety. Further, the differential expression of key genes in the ABAregulated stress response pathway, i.e., pyrabactin resistance (PYR)/PYR-Like (PYL)/regulatory components of ABA receptors (RCAR), protein phosphatase 2C (PP2C), sucrose non-fermenting 1-related kinase (SnRK2) and specific ABC transporters, was analysed by quantitative real-time PCR of leaf RNA. The PP2CA and ABC transporters were up-regulated while the PYR/PYL/RCARs and SnRK2s were down-regulated under salt stress. Importantly, tolerance ranking by gene expression closely correlated that by physiological indices. Thus expression analysis of the ABA pathway may be used for rapid identification of potentially salt-tolerant barley varieties before undertaking physiological studies. The outcomes are relevant for varietal selection and comparing the alleles for genetics of tolerance.

Analysis of the barley leaf transcriptome under salinity stress using mRNA-Seq

76%

Ziemann M., Kamboj A., Hove R. M., Loveridge S., El-Osta A., Bhave M.

Acta Physiologiae Plantarum

2013

tom 35

nr 06

Salinity is a threat to crops in many parts of the world, and together with drought, it is predicted to be a serious constraint to food security. However, understanding the impact of this stressor on plants is a major challenge due to the involvement of numerous genes and regulatory pathways. While transcriptomic analyses of barley (Hordeum vulgare L.) under salt stress have been reported with microarrays, there are no reports as yet of the use of mRNA-Seq. We demonstrate the utility of mRNA-Seq by analysing cDNA libraries derived from acutely salt-stressed and unstressed leaf material of H. vulgare cv. Hindmarsh. The data yielded[50 million sequence tags which aligned to 26,944 sequences in the Unigene reference database. To gain maximum information, we performed de novo assembly of unaligned reads and discovered[3,800 contigs, termed novel tentative consensus sequences, which are either new, or significant improvements on current databases. Differential gene expression screening found 48 significantly up-regulated and 62 significantly down-regulated transcripts. The work provides comprehensive insights into genome-wide effects of salinity and is a new resource for the study of gene regulation in barley and wheat. Further, the bioinformatics workflow may be applicable to other non-model plants to establish their transcriptomes and identify unique sequences.

Ograniczanie wyników

2 Acta Physiologiae Plantarum

2 Bhave M.

2 Kamboj A.

2 Ziemann M.

1 El-Osta A.

1 Hove R. M.

1 Loveridge S.

1 2015

1 2013

Wyniki wyszukiwania

Identification of salt-tolerant barley varieties by a consolidated physiological and molecular approach

Analysis of the barley leaf transcriptome under salinity stress using mRNA-Seq