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Application of specific primers in the diagnosis of Encephalitozoon spp.

100%

Valencakova A., Balent P., Novotny F., Cislakova L.

Annals of Agricultural and Environmental Medicine

|

2005

|

tom 12

|

nr 2

321-323

In our experiment, 3 species-specific primer pairs cultivated in cell lines were used: Encephalitozoon cuniculi-specific primer pairs (ECUNF and ECUNR), Encephalitozoon hellem-specific primer pairs (EHELF and EHELR), and Encephalitozoon intestinalis-specific primer pairs (SINTF and SINTR). The PCR products were estimated to be 550 bp in E. cuniculi, 547 bp in E. hellem and 545 bp in E. intestinalis respectively, which can prove the precision and reliability of this method in the species identification of the genus Encephalitozoon. All 3 primer pairs were species-specific and none of them amplified gene sequences from other Encephalitozoon spp.

2

Occurrence of antibodies against Chlamydophila abortus in sheep and goats in the Slovak Republic

100%

Cislakova L., Halanova M., Kovacova D., Stefancikova A.

Annals of Agricultural and Environmental Medicine

|

2007

|

tom 14

|

nr 2

Chlamydophila abortus is one of the most important causative agents of enzootic abortion and other chlamydial infections of sheep and goats. The presence of specifi c serum antibodies to Chlamydophila abortus was studied in sheep and goats breeding in the Slovak Republic by the complement fi xation test. 22,040 sheep and goats were examined during 5 years. Specifi c anti-Ch. abortus antibodies were found in 2,360 out of 20,878 sheep sera examined (11.7%), and in 85 out of 1,162 examined goats (7.7%). The occurrence of antichlamydial antibodies indicates the importance of performing screening examinations in commercial breeding with the aim of reducing the spread of this disease between animals, and also interrupting the spread and transmission from animals to human.

3

Occurrence of IgG antibodies to Anaplasma phagocytophilum in humans suspected of Lyme borreliosis in Eastern Slovakia

88%

Kalinova Z., Halanova M., Cislakova L., Sulinova Z., Jarcuska P.

Annals of Agricultural and Environmental Medicine

|

2009

|

tom 16

|

nr 2

285-288

4

Occurrence of antibodies to Anaplasma phygocytophilum in patients with suspected tick-borne encephalitis

88%

Kalinova Z., Halanova M., Cislakova L., Juris P., Cechova L.

Annals of Agricultural and Environmental Medicine

|

2015

|

tom 22

|

nr 3

5

Encephalitozoon cuniculi infection in rabbits and laboratory mice in Eastern Slovakia

88%

Balent P., Halanova M., Sedlakova T., Valencakova A., Cislakova L.

Bulletin of the Veterinary Institute in Puławy

|

2004

|

tom 48

|

nr 2

6

Wild pigeons and pheasants - a source of Chlamydophila psittaci for humans and animals

88%

Travnicek M., Cislakova L., Deptula W., Stosik M., Bhide M. R.

Annals of Agricultural and Environmental Medicine

|

2002

|

tom 09

|

nr 2

7

Serological screening of selected microsporidia in HPV-positive women

76%

Adam J., Valencakova A., Halanova M., Danisova O., Gorbarova K., Cislakova L.

Acta Parasitologica

|

2015

|

tom 60

|

nr 1

8

Badania serologiczne w kierunku boreliozy i erlichiozy swin i krow na Lubelszczyznie

76%

Winiarczyk S., Adaszek L., Stefancikova A., Pet'ko B., Cislakova L., Puchalski A.

Medycyna Weterynaryjna

|

2007

|

tom 63

|

nr 05

561-565

The aim of this paper was the detection of the specific antibodies anti-Borrelia and anti-Ehrlichia in the sera of 28 sick pigs and 29 sick cows from the area of the Lublin voivodeship. ELISA tests, and Western blot were conducted. In ELISA tests, 17.86% sera of swine were positive for spirochetes and 7.14% for Ehrlichia. In a bovine group 44.83% of sera specimens were positive for Borrelia, and 20.69% for Ehrlicha. All sera that were positive in ELISA tests for spirochetes were examined additionally in Western blot method for the presence of antibodies against Borrelia afzelii (13 bovine sera, and 5 swine sera). In 8 out of 13 bovine sera (61.54%) and in one out of 5 (20%) swine sera that were positive in the ELISA test, specific antibodies against Borrelia were detected. In all cases IgG immunoglobins reacted strongly with the antigens: 31, 39, 41, 66 kDa of B. afzelii. The results revealed a low specificity of the ELISA test for the detection of borreliosis and confirmed an endemic occurrence of Borrelia and Ehrlicha in the area of the Lublin voivodeship

9

Serological response of cattle to Chlamydophila abortus in Slovakia in 1996-2000

76%

Travnicek M., Kovacova D., Deptula W., Bajova V., Cislakova L., Zubricky P., Stosik M.

Polish Journal of Veterinary Sciences

|

2002

|

tom 05

|

nr 1

10

Heterogeneity of Borrelia burgdorferi sensu lato and their reflection on immune response

76%

Stefancikova A., Derdakova M., Stepanova G., Pet'ko B., Szestakova E., Skardova I., Cislakova L.

Annals of Agricultural and Environmental Medicine

|

2005

|

tom 12

|

nr 2

211-216

Geographically different strains of Borrelia burgdorferi sensu lato (B. burgdorferi sensu stricto Ir 105, B. burgdorferi s.s. + B. afzelii V 123, B. garinii Ir 112 - isolates from eastern Slovakia, B. garinii K24 - isolate from western Slovakia and B. burgdorferi s.s. B 31 - American strain) were compared as antigens for serological study of Lyme borreliosis by IgG ELISA on a group of horses from eastern Slovakia. In a set of 101 horse serum samples, positivity with the use of Ir 105 strain was 53 (52.4%), with V 123 51 (51.49%), with Ir 112 48 (47.5%), with K 24 47 (46.5%) and with B 31 only 25 (24.7%). The seroprevalence between strains B 31 and Ir 105, B 31 and V 123, B31 and Ir112, B 31 and K 24 differed statistically significantly ( test chi2, p<0.05); however, the differences between strains Ir 105, V 123, Ir 112 and K 24 were insignificant. Consistency of positive and negative findings between American and Slovak strains ranged from 50.5-62.4%. Comparison of Slovak strains (Ir 105, V 123, Ir 112 and K 24) consistency of positive and negative findings was higher from 79.2-95.04% The highest consistency of findings was reached comparing strains Ir 112 and K 24, and the same high agreement of results was observed between the strains Ir 105 and V 123 and also Ir 112 and Ir 105. Higher consistency of findings of serologically examined horses with geographically close strains is in accordance with greater similarity of protein profiles of Slovak strains compared to the American strain.

11

Detection of CHLAMYDIA PSITTACI in feral pigeons (COLUMBA LIVIA DOMESTICA) in Slovakia and their characterisation

76%

Cechova L., Halanova M., Kalinova Z., Cislakova L., Halan M., Valencakova A.

Annals of Agricultural and Environmental Medicine

|

2016

|

tom 23

|

nr 1

12

Occurrence of microsporidia as emerging pathogens in Slovak Roma children and their impact on public health

76%

Halanova M., Valencakova A., Kvac M., Sak B., Kvetonova D., Balent P., Cislakova L.

Annals of Agricultural and Environmental Medicine

|

2013

|

tom 20

|

nr 4

13

Serological screening of occurrence of antibodies to Encephalitozoon cuniculi in humans and animals in Eastern Slovakia

76%

Halanova M., Cislakova L., Valencakova A., Balent P., Adam J., Travnicek M.

Annals of Agricultural and Environmental Medicine

|

2003

|

tom 10

|

nr 1

14

Molecular evidence for Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from Eastern Slovakia

76%

Derdakova M., Halanova M., Stanko M., Stefancikova A., Cislakova L., Petko B.

Annals of Agricultural and Environmental Medicine

|

2003

|

tom 10

|

nr 2

15

Prevalence of antibodies to Encephalitozoon cuniculi [Microsporidia] in angora goats - a potential risk of infection for breeders

76%

Cislakova L., Literak I., Balent P., Hipikova V., Levkutova M., Travnicek M., Novotna A.

Annals of Agricultural and Environmental Medicine

|

2001

|

tom 08

|

nr 2

16

Chlamydiosis in farmed chickens in Slovakia and zoonotic risk for humans

64%

Cechova L., Halanova M., Babinska I., Danisova O., Bartkovsky M., Marcincak S., Marcincakova D., Valencakova A., Cislakova L.

Annals of Agricultural and Environmental Medicine

|

2018

|

tom 25

|

nr 2

17

Small mammals [Insectivora, Rodentia] as a potential source of chlamydial infection in East Slovakia

64%

Cislakova L., Stanko M., Fricova J., Mosansky L., Travnicek M., Halanova M., Mardzinova S., Stefancikova A.

Annals of Agricultural and Environmental Medicine

|

2004

|

tom 11

|

nr 1

The presence of antibodies against Chlamydophila (Chlamydia) psittaci in small mammals (Insectivora, Rodentia) in the region of East Slovakia are presented. The hosts were caught in several areas of Slovakia in habitats with different levels of anthropogenic disturbance. Research was carried out during 2000-2002. The authors examined 1,947 sera coming from 4 insectivore and 10 rodent species. Each serum was examined by micromethod of complement binding reactions using antigen Chlamydophila (Chlamydia) psittaci. Chlamydial infections were found in 251 individuals (prevalence 12.9%) of 8 mammal species. The antichlamydial antibodies were proved at levels ranging from 1:32-1:1024. The highest prevalence of antibodies was detected in the most abundant rodent species Apodemus microps (14.8%), Apodemus agrarius (13.9%), Apodemus flavicolis (12.4%), Microtus arvalis (12%), and Clethrionomys glareolus (10.9%). Positive hosts were registered in all studied localities. Testing of prevalence values in the individual research years confirmed significant changes. Our results showed that small mammals probably play an important role in the circulation of chlamydiae in nature.

18

Seroprevalence of anti-Borrelia burgdorferi antibodies in sheep and goats from mountainous areas of Slovakia

64%

Travnicek M., Stefancikova A., Nadzamova D., Stanko M., Cislakova L., Pet'ko B., Mardzinova S., Bhide M. R.

Annals of Agricultural and Environmental Medicine

|

2002

|

tom 09

|

nr 2

19

Epidemiology of Lyme borreliosis in Slovakia

52%

Pet'ko B., Tresova G., Stefancikova A., Peterkova J., Prokopcakova H., Cislakova L., Stanko M., Fricova J., Skardova I., Sesztakova E., Nadzamova D.

Wiadomości Parazytologiczne

|

1998

|

tom 44

|

nr 3

20

Occurrence of Echinococcus multilocularis and E. granulosus seropositivity in Roma and non-Roma population of Eastern Slovakia

34%

Antolova D., Jarcuska P., Janicko M., Reiterova K., Halanova M., Cechova L., Cislakova L., Geckova A. M., Marekova M., Pella D., Siegfried L., Pastvova L., Fedacko J., Kollarova J., Kolarcik P., Bobakova D., Veselska Z., Babinska I., Drazilova S., Rosenberger J., Schreter I., Kristian P., Veseliny E., Virag L., Birkova A., Kmet'ova M., Petrasova D., Carikova K., Lovayova V., Merkovska L., Jedlickova L., Valkova I.

Annals of Parasitology

|

2016

|

tom 62

|

nr Suppl.

Ograniczanie wyników

Application of specific primers in the diagnosis of Encephalitozoon spp.

Occurrence of antibodies against Chlamydophila abortus in sheep and goats in the Slovak Republic

Occurrence of IgG antibodies to Anaplasma phagocytophilum in humans suspected of Lyme borreliosis in Eastern Slovakia

Occurrence of antibodies to Anaplasma phygocytophilum in patients with suspected tick-borne encephalitis

Encephalitozoon cuniculi infection in rabbits and laboratory mice in Eastern Slovakia

Wild pigeons and pheasants - a source of Chlamydophila psittaci for humans and animals

Serological screening of selected microsporidia in HPV-positive women

Badania serologiczne w kierunku boreliozy i erlichiozy swin i krow na Lubelszczyznie

Serological response of cattle to Chlamydophila abortus in Slovakia in 1996-2000

Heterogeneity of Borrelia burgdorferi sensu lato and their reflection on immune response

Detection of CHLAMYDIA PSITTACI in feral pigeons (COLUMBA LIVIA DOMESTICA) in Slovakia and their characterisation

Occurrence of microsporidia as emerging pathogens in Slovak Roma children and their impact on public health

Serological screening of occurrence of antibodies to Encephalitozoon cuniculi in humans and animals in Eastern Slovakia

Molecular evidence for Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from Eastern Slovakia

Prevalence of antibodies to Encephalitozoon cuniculi [Microsporidia] in angora goats - a potential risk of infection for breeders

Chlamydiosis in farmed chickens in Slovakia and zoonotic risk for humans

Small mammals [Insectivora, Rodentia] as a potential source of chlamydial infection in East Slovakia

Seroprevalence of anti-Borrelia burgdorferi antibodies in sheep and goats from mountainous areas of Slovakia

Epidemiology of Lyme borreliosis in Slovakia

Occurrence of Echinococcus multilocularis and E. granulosus seropositivity in Roma and non-Roma population of Eastern Slovakia