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In a study with 12 calves on milk nutrition the course of methemoglobinemia as well as transrenal passage of nitrates and nitrites after a single per os administrations of 4 g NaNO₂ per animal and 30 g KNO₃ per animal in the form of water solutions has been observed. The response of the organism of calves to per os administered doses of sodium nitrite and potassium nitrate was observed by the determination of the methemoglobin percentage in blood and the nitrate and nitrite content in urine before the administration of the respective dose and after 1, 2, 3 and 4 h after the administration. A significant elevation in the values of methemoglobin was recorded 2 h after the administration of 4 g of NaNO₂ per animal. The mean value of methemoglobin in blood was 18.84% of total hemoglobin. A slight decline in the values occurred as early as 3 h after the administration. Of clinical signs, cyanosis of visible mucosae was observed. The highest nitrite and nitrate values in urine were determined 2 h after per os administration of 4 g of NaNO₂. With the administration of 30 g KNO₃ per animal, the most pronounced elevation in methemoglobinemia was observed after 3 h, when the mean values of methemoglobin was 11.75%. Of clinical signs, only slight cyanosis of mucosae was detectable. Maximum values of nitrates in urine of experimental calves 3 h after the administration of 30 g KNO₃ per animal, with the mean value of 29.9 mM NO₃⁻ clearly demonstrate a good transrenal passage of nitrates in calves on milk nutrition.
Effect of both vitamin D₃ and cadmium (Cd) on chymotrypsin activity (ACHT) of laying hen has been studied. Ten month-old laying hens were fed a compound feed of HYD-10 ad libitum. The birds were divided into 8 groups (each group consisting of 6 birds) as follows: experimental groups G1, G3, and G5 were supplemented with Cd (0.3; 0.6; and 6.0 mg.kg⁻¹ CdCl₂, respectively) groups G2, G4, and G6 were supplemented with vitamin D₃ (100 I.U./day) at the same Cd concentrations. C₁ was control group without any supplements and C₂ was control group supplemented only with vitamin D₃. The study lasted 6 months. In groups G1-G4, ACHT activities in the end of the study were rather high (2097.4; 2984; 1805.4, and 2575.9 U.g⁻¹,respectively). The ACHT activity in groups G2, G4 and G6 was by little higher than that in groups G1, G3, and G5 without vitamin D₃ supplementation. The principle of the action of vitamin D₃ via Ca ions and calmodulin is further discussed.
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