Ograniczanie wyników

Czasopisma help
Autorzy help
Lata help
Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 31

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
A phylogenetic tree shows graphically the evolutionary relationships among various organisms. The dynamic development of molecular biology and bioinformatics has led to a revolution in our knowledge of biological evolution and the kinships between living organisms and viruses. Nowadays, the available laboratory techniques and computer software allow reconstruction of the actual changes which occurred in the evolutionary process. The derivation of molecular evolution models and several methods for building phylogenetic trees have played a huge role in that enterprise. The emergence of new infectious agents is a problem afflicting mankind since prehistoric times. The study of phylogenetic implications among pathogenic microorganisms allows tracking the process of evolution, the indirect understanding of their biology, and thus facilitates the implementation of treatment. The presented article demonstrates the basic methods for constructing phylogenetic trees, as well as the benefits of reconstructing the evolution process and kinship with the study of microorganisms; in particular, viruses are considered from the clinical aspect.
The aim of this study was to compare the prevalence of EBV genotype and del-LMP-1 in saliva from Polish, Taiwanese and Arabic healthy students. The study group consisted of 56 healthy students; 24 of them Polish, 25 Taiwanese, and 7 Arabic. Typing was carried out using PCR with EBNA-2 primers. A detection of LMP-1 variants was also performed using PCR. EBV DNA was detected in 22 investigated samples (39.3%). Type 1 of the virus was dominant in both Polish and Taiwanese group. Among 62.5% Tai wanese with EBV 1 and 55.6% Polish detected EBV with 30-bp deletion in LMP-1 gene.
The aim of this study was the analysis of the prevalence of Herpesviridae (EBV, CMV, HSV-1) among patients with head and neck cancers (larynx and hypopharynx squamous cell carcinoma). Correlation between viral infection, HNSCC, alcohol use, tobacco smoking, demographic data (gender, age, place of residence, occupation), anatomic location, pre-treatment staging, evidence of metastases to lymph nodes, and grading was also investigated. The examination samples were collected from paraffin-blocks tissues, from 35 patients. The laboratory part of investigation involved: isolation of DNA from histopathological specimens, qualitative analysis of DNA comprising of amplification of human ß-globin gene (reference gene) fragment, electrophoresis of amplification products of the gene fragment, quantitative DNA analysis (using spectrophotometric method), and viral DNA amplified by the nested-PCR method. There was a statistically significant correlation between T and tobacco smoking, and T and alcohol use. There was no statistically significant correlation between the presence of EBV, CMV, HSV-1 and G and epidemiological features such as place of residence.
Head and neck cancers constitute the sixth most common malignant tumours worldwide, and are one of the majors problems of global public health. In the oral cavity there are several types of oral cancers, but around 90% are squamous cell carcinoma. Many different risk factors play a role in the etiology of head and neck cancer. The aim of this study is presentation from the aspect of molecular, viral and bacterial infection. Knowledge about all factors which influence the development of these malignances is essential for diagnostics and successful treatment.
The aim of this study was to analyse the prevalence of human papillomavirus (HPV) infection among patients with oral and oropharynx squamous cell carcinoma (OSCC). The correlation between HPV infection and OSCC, HPV genotypes, and correlation between HPV, OSCC, alcohol use, tobacco smoking, demographic data (gender, age, place of residence), anatomic location, pretreatment staging, metastases of lymph node evidence, and grading was investigated. In the examination group, there were 60 patients with squamous cell carcinoma (SCC), 54 males and six females. Twenty-one patients were affected with oral, 24 - with oropharynx, and 15 - with oral and oropharynx SCC. The patients were not subjected to chemotherapy and radiotherapy before operation. The examination samples were collected from paraffin sections. This analysis involved DNA isolation from histological specimens, an amplification of a fragment of human ß-globin gene (reference gene), electrophoresis of amplification products of the gene, a quantitative DNA analysis (using spectrophotometry method), PCR, and genotyping. HPV DNA was detected in 25% of patients with SCC. Among HPV positive patients, 86.7% of the patients were infected with HPV type 16 and 13.3% were infected with another non identified HPV.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.