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Regulacja funkcji sekrecyjnej komórek jajnika świń w czasie cyklu estralnego, ciąży i ciąży rzekomej

100%

Przala J.

Zeszyty Problemowe Postępów Nauk Rolniczych

|

1987

|

tom 339

2

Retikulocytoza jako wskaźnik aktywności erytropoezy w świetle badań nad czynnikami osocza wpływającymi na dojrzewanie retikulocytów

63%

Krzymowska H., Przala J.

Acta Physiologica Polonica

|

1965

|

tom 16

|

nr 3

3

The effect of oxytocin [OT] and VIP on the PRL and LH secretion by porcine pituitary cells after treatment with exogenous progesterone

63%

Bogacka I., Przala J.

Folia Histochemica et Cytobiologica. Supplement

|

1997

|

tom 35

|

nr 2

4

The involvement of catecholamines in the pituitary release of luteinizing hormone [LH] and prolactin [PRL] in progesterone [P4] - treated gilts

63%

Siawrys G., Przala J.

Folia Histochemica et Cytobiologica. Supplement

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1997

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tom 35

|

nr 2

5

Long form of leptin receptor gene and protein expression in the porcine ovary during the estrous cycle and early pregnancy

51%

Smolinska N., Kaminski T., Siawrys G., Przala J.

Reproductive Biology

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2007

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tom 07

|

nr 1

6

Wpływ estradiolu 17 beta (E2) i hormonu luteinizującego LH na sekrecję progesteronu (P4) przez komórki lutealne pochodzące od loch w ciąży i w ciąży rzekomej in vitro

51%

Grazul A., Przala J., Wiesak T.

Zeszyty Problemowe Postępów Nauk Rolniczych

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1984

|

tom 309

7

Wpływ oksytocyny na komórki lutealne świń z wczesnego okresu ciąży

51%

Przala J., Grazul A., Wiesak T.

Zeszyty Problemowe Postępów Nauk Rolniczych

|

1984

|

tom 309

8

Influence of PGF2a on progesterone synthesis by porcine corpus luteum in vitro

51%

Przala J., Krzymowski T., Grazul A.

Acta Physiologica Polonica

|

1979

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tom 30

|

nr 1

9

Wpływ hormonu luteinizującego (LH), prolaktyny (PRL) i hCG na sekrecję estradiolu 17 beta przez komórki lutealne świń z wczesnego okresu ciąży

51%

Przala J., Wiesak T., Grazul A.

Zeszyty Problemowe Postępów Nauk Rolniczych

|

1984

|

tom 309

10

Effect of LH and prolactin on steroid secretion by perifused luteal tissue from pregnant gilts with induced hypoprolactinemia or after passive immunoneutralization of LH

51%

Szafranska B., Grazul-Bilska A., Przala J.

Archivum Veterinarium Polonicum

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1992

|

tom 32

|

nr 1-2

1-15

11

Expression of orexin receptors 1 [OX1R] and 2 [OX2R] in the porcine hypothalamus during the oestrous cycle

51%

Kaminski T., Smolinska N., Nitkiewicz A., Przala J.

Journal of Physiology and Pharmacology

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2010

|

tom 61

|

nr 3

363-371

Orexin A and B are hypothalamic peptides involved in the control of food intake, sleep patterns, autonomic and neuroendocrine systems. The biological actions of orexins are mediated via two G-protein coupled receptors, termed orexin receptor 1 (OX1R) and orexin receptor 2 (OX2R). The present study analyzed mRNA and protein expressions of OX1R and OX2R in porcine hypothalamic structures engaged in GnRH production and secretion, preoptic area (POA), mediobasal hypothalamus (MBH), and stalk median eminence (SME) on days 2-3, 10-12, 14-16, and 17-19 of the oestrous cycle. The highest OX1R gene expression in POA was observed on days 17-19 of the cycle. Changes in the mRNA expression in MBH and SME throughout the cycle were negligible. The expression peak of OX2R gene in POA and SME occurred on days 17-19 as well. There were no changes in the gene expression in MBH. OX1R protein content in POA and SME also was the greatest on days 17-19 and OX2R protein expression was most pronounced in MBH and SME during the same phase of the cycle. In conclusion, fluctuation of OX1R and OX2R mRNAs and proteins content in pig hypothalamus dependently on the phase of the oestrous cycle suggests that orexins, through the influence on the hypothalamic-pituitary-ovarian axis, may affect reproductive functions.

12

Profilaktyczne zastosowanie preparatu Biolactin-2 u loszek remontowych a syndrom MMA

51%

Przala J., Gajecki M., Przala F., Ryszka F.

Medycyna Weterynaryjna

|

1992

|

tom 48

|

nr 01

31-33

The effect of Biolactin-2 preparation, containing 5 mg of purified prolactin, on the occurrence of MMA syndrome and the number and weight of piglets in the litter was studied. The study was carried out on 212 gilts of which 150 were prophylactically administered Biolactin-2 preparation. Clinical examinations showed that MMA syndrome occured only in 7,33% of gilts in the group receiving Biolactin-2, while in the control group, it occured in 41,9%. Moreover, a higher body weight of the litter of 5.24 kg and larger litter size of 1.7 piglet, was ascertained at day 10 after birth amoung those given the preparation.

13

Ontogeny of the long form of leptin receptor gene expression in the porcine ovarian follicles

51%

Smolinska N., Kaminski T., Siawrys G., Przala J.

Polish Journal of Veterinary Sciences

|

2013

|

tom 16

|

nr 1

Leptin is a polypeptide hormone produced predominantly in adipocytes. It has been found to be implicated in the regulation of satiety and energy homeostasis. A role for leptin in reproduction was later suggested by findings that this hormone may be involved in the regulation of the hypothalamic- pituitary-gonadal axis via endocrine, paracrine and/or autocrine pathways. The objective of the study was to investigate the ontogeny of the long isoform of leptin receptor (OB-Rb) gene in porcine ovarian follicles. The expression of OB-Rb gene was detected in porcine primordial, primary, secondary and antral follicles by in situ hybridization. In summary, our data suggest that leptin might have a direct effect on porcine follicles and plays an important role in the follicular development.

14

Expression of leptin and long form of leptin receptor genes and proteins in pituitary of cyclic and pregnant pigs

51%

Siawrys G., Kaminski T., Smolinska N., Przala J.

Journal of Physiology and Pharmacology

|

2007

|

tom 58

|

nr 4

Leptin is a multifunctional regulator in numerous tissues, including the pituitary. It is not known, whether the porcine pituitary is a source of leptin synthesis and possesses the leptin receptor protein. It is also unknown, if a relationship exists between expression levels of these proteins in the pituitary and physiological status of sows. Therefore, the aim of the study was 1] to examine, by Western-blotting analysis, the expression levels of leptin and the long form of leptin receptor (OB-Rb) in the porcine anterior (AP) and posterior (NP) pituitary gland during mid- and late-luteal phases of the oestrous cycle (days 10 - 12 and 14 - 16) as well as during two stages of early pregnancy (days 14 - 16 and 30 - 32); and 2] to localise, using in situ hybridisation method (ISH), the expression of leptin and OB-Rb genes in the pituitary gland in the above mentioned stages of the cycle and pregnancy. Western-blotting analysis showed that leptin protein expression in AP was higher in the late-luteal phase than in the mid-luteal phase, while OB-Rb protein expression in both lobes was higher in the mid-luteal phase. In turn, during pregnancy leptin protein content in AP and OB-Rb protein content in NP were more pronounced on days 14 - 16 than on days 30 - 32. Comparison of leptin and OB-Rb protein expression levels in AP between the mid-luteal phase and two periods of pregnancy showed, respectively, stimulation of leptin protein and inhibition of OB-Rb protein expressions during both examined stages of pregnancy. Taking AP from late-luteal phase as the point of reference, it was revealed stimulation of leptin expression during earlier period of pregnancy, whereas on days 30 - 32 of pregnancy both the hormone and its receptor expressions were diminished. In turn, comparison of leptin and OB-Rb protein expression levels in NP between the late-luteal phase and days 14 - 16 or 30 - 32 of pregnancy showed inhibition of leptin protein expression and stimulation of OB-Rb protein expression during pregnancy. Moreover, ISH studies localised leptin and OB-Rb mRNAs expression in the cells of AP as well as NP tissue during the two stages of the cycle and pregnancy. In conclusion, our findings suggest that leptin is produced within the pituitary in the pig and may participate in auto/paracrine manner in the regulation of this gland function during the luteal phase of the oestrous cycle and early pregnancy.

15

The role of opioids in porcine corpus luteum function

51%

Przala J., Kaminski T., Bogacka I., Siawrys G.

Journal of Physiology and Pharmacology. Supplement

|

1996

|

tom 47

|

nr 1

16

Leptin gene and protein expression in the trophoblast and uterine tissues during early pregnancy and the oestrous cycle of pigs

51%

Smolinska N., Siawrys G., Kaminski T., Przala J.

Journal of Physiology and Pharmacology

|

2007

|

tom 58

|

nr 3

Leptin is a 16-kDa protein hormone encoded by the obese (ob) gene and acts on receptors in the hypothalamus to regulate food intake and energy balance. The identification of leptin and its receptor mRNAs and proteins in human and mouse endometrium and placental trophoblast has attracted attention to the potential role of leptin in implantation. Thus, the aim of this study was to compare the expression levels of porcine leptin mRNA and protein in endometrium and myometrium during mid- and late-luteal phases of the oestrous cycle (days 10 - 12 and 14 - 16) as well as during two stages of pregnancy respondent to the beginning (days 14 - 16) and the end (days 30 - 32) of the implantation process, and in trophoblast during both periods of pregnancy. Leptin gene and protein expression in myometrium, and leptin mRNA expression in endometrium was more pronounced in the mid- and late-luteal phases of the cycle in comparison to studied periods of pregnancy, whereas leptin protein concentration in endometrium was either enhanced on days 30 - 32 of pregnancy in relation to days 14 - 16 of the cycle or there were no changes between pregnancy and luteal phase of the cycle. On days 30 - 32 of pregnancy, expression of the leptin gene in the endometrium, and of the leptin gene and protein in the myometrium was more pronounced in comparison to the earlier stage of pregnancy. Moreover, leptin gene expression in porcine trophoblast increased during the beginning of the implantation process compared to days 30 - 32 of pregnancy, while the protein concentration decreased on days 14 - 16 of pregnancy. In conclusion, the finding of leptin gene and protein expression in porcine endometrium, myometrium and trophoblast indicates that locally synthesised leptin can participate in the control of pig reproduction. The fluctuation of the hormone concentration during pregnancy and changes in its level between pregnancy and the oestrous cycle may indicate leptin's involvement in the implantation process.

17

Cyclic AMP and cGMP production by isolated small and large porcine luteal cells - effect of hCG and TNFalpha

51%

Przala J., Bogacka I., Siawrys G.

Folia Histochemica et Cytobiologica. Supplement

|

1997

|

tom 35

|

nr 2

18

Czynniki wzrostowe w jajniku

51%

Kaminski T., Przala J.

Postępy Biologii Komórki

|

1994

|

tom 21

|

nr 1

79-92

19

Udzial opioidow w lokalnej regulacji funkcji jajnika

45%

Przala J., Kaminski T., Okrasa S., Siawrys G., Bogacka I.

Postępy Biologii Komórki. Suplement

|

1999

|

tom 12

113-116

20

The involvement of adenylyl cyclase and protein kinase A in the mechanism of opioid peptide action in porcine theca interna cells

45%

Kaminski T., Siawrys G., Bogacka I., Okrasa S., Przala J.

Cellular and Molecular Biology Letters

|

2002

|

tom 07

|

nr Suppl.

Ograniczanie wyników

Regulacja funkcji sekrecyjnej komórek jajnika świń w czasie cyklu estralnego, ciąży i ciąży rzekomej

Retikulocytoza jako wskaźnik aktywności erytropoezy w świetle badań nad czynnikami osocza wpływającymi na dojrzewanie retikulocytów

The effect of oxytocin [OT] and VIP on the PRL and LH secretion by porcine pituitary cells after treatment with exogenous progesterone

The involvement of catecholamines in the pituitary release of luteinizing hormone [LH] and prolactin [PRL] in progesterone [P4] - treated gilts

Long form of leptin receptor gene and protein expression in the porcine ovary during the estrous cycle and early pregnancy

Wpływ estradiolu 17 beta (E2) i hormonu luteinizującego LH na sekrecję progesteronu (P4) przez komórki lutealne pochodzące od loch w ciąży i w ciąży rzekomej in vitro

Wpływ oksytocyny na komórki lutealne świń z wczesnego okresu ciąży

Influence of PGF2a on progesterone synthesis by porcine corpus luteum in vitro

Wpływ hormonu luteinizującego (LH), prolaktyny (PRL) i hCG na sekrecję estradiolu 17 beta przez komórki lutealne świń z wczesnego okresu ciąży

Effect of LH and prolactin on steroid secretion by perifused luteal tissue from pregnant gilts with induced hypoprolactinemia or after passive immunoneutralization of LH

Expression of orexin receptors 1 [OX1R] and 2 [OX2R] in the porcine hypothalamus during the oestrous cycle

Profilaktyczne zastosowanie preparatu Biolactin-2 u loszek remontowych a syndrom MMA

Ontogeny of the long form of leptin receptor gene expression in the porcine ovarian follicles

Expression of leptin and long form of leptin receptor genes and proteins in pituitary of cyclic and pregnant pigs

The role of opioids in porcine corpus luteum function

Leptin gene and protein expression in the trophoblast and uterine tissues during early pregnancy and the oestrous cycle of pigs

Cyclic AMP and cGMP production by isolated small and large porcine luteal cells - effect of hCG and TNFalpha

Czynniki wzrostowe w jajniku

Udzial opioidow w lokalnej regulacji funkcji jajnika

The involvement of adenylyl cyclase and protein kinase A in the mechanism of opioid peptide action in porcine theca interna cells