The aim of this study was to describe the incidence of bacteria and fungi in the milk of dairy cows at the moment of drying off in 8 herds in North-East Poland. Additionally, tests for antibiotic sensitivity of the isolated bacteria were performed. In total, milk samples of 1145 quarters of 288 cows were examined. In 41 cows coagulase negative staphylococci (CNS) were isolated, while Strep. agalactiae was isolated in 22 cows, Strep. uberis in 18 cows, Staph. aureus in 16 cows, E. coli in 10 cows, Strep. dysgalactiae in 3 cows, and Arcanobacter pyogenes in 1 cow. Fungal infections were found in 28 cows. The lowest level of antibiotic resistance among the isolated CNS strains was found for cefquinome, Nafpenzal® and cephalexin while the highest number of resistant strains was found when novobiocin and streptomycin were tested. For Strep. agalactiae the lowest resistance level could be found when cefquinome and cephoperazone were tested, while the highest level was found for streptomycin and kanamycin. When testing Strep. - uberis the less resistance was found for cefquinome, cephalexin and Nafpenzal® and the highest for streptomycin and kanamycin. Staph. aureus strains were mostly sensitive for amoxicillin with clavulanic acid and Nafpenzal®, while streptomycin and neomycin were found to have the highest resistance level. The results of the present study show that bacteria can be isolated in the milk of one third of the cows at the moment of drying off and that resistance levels among the isolated bacteria were lowest for cephalosporins.
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