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The biopolymer chitosan is a derivative of chitin, which can promote plant growth and protect plants from phytopathogens. This study aimed to evaluate the efficacy of chitosan as a biostimulant and a biorational agent to protect cucumber plants from damping-off disease caused by Phytophthora capsici. Cucumber seeds were treated with a range of chitosan concentrations, viz. 0, 125, 250, and 500 ppm, to evaluate effect on seed germination and fresh root and shoot weight of the seedlings. Chitosan significantly (p ≤ 0.05) enhanced seed germination and root and shoot growth of cucumber in a dose-dependent manner up to 500 ppm. Application of in vitro chitosan suspension onto P. capsici mycelial plug suppressed growth of mycelia, formation of sporangia, and release of P. capsici zoospores at 125–500 ppm concentrations. Cucumber seedlings from chitosan-treated seeds showed enhanced resistance to damping-off disease caused by P. capsici compared to untreated control. Cucumber seedlings from 500 ppm chitosan seed treatment showed 100% disease resistance against damping off caused by P. capsici. These results suggest that chitosan could be used as a natural and environmentally safe alternative to a synthetic growth promoter and pesticide for sustainable production of cucumber.
While phosphate (Pi) serves as an essential and indispensible plant nutrient, phosphite (Phi) acts as a potent herbicide. Despite their differential influence on plants, both the ions can attenuate phosphate starvation responses (PSRs). We analyzed and compared Pi and Phi uptake and accumulation, attenuation of PSRs and the morphological and physiological responses of the rice seedlings in response to the increasing concentrations of Pi and Phi. Our study revealed that increasing levels of Phi led to pronounced reduction in shoot and root mass in rice seedlings in comparison to similar Pi treatments. Phi inhibited root hair and root formation at 5 and 30 mM Phi concentrations, respectively. Whereas, higher Pi concentrations (40 and 50 mM) affected only root hair elongation. Increasing Phi dose led to drastic reduction in chlorophyll content which was not so in case of Pi. There was inverse relationship between external Pi/Phi level and anthocyanin content of the leaves. In comparison to 20 mM Pi treatment, similar dose of Phi led to significant downregulation of Pi transportersin both leaves and roots. Rice seedlings were found to accumulate mmol and lmol levels of Pi and Phi, respectively. Comparison of various PSR parameters revealed that in comparison to Pi, Phi exhibited greater degree of attenuation of PSRs. Lesser Phi accumulation and greater attenuation of PSRs by Phi indicate plant’s adaption to restrict entry of this toxic ion inside cells.
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