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Cytokinin overproducing ipt-161 Arabidopsis shows altered NO generation and insensitivity to selenite

100%

Kolbert Z., Lehotai N., Peto A., Feigl G., Tugyi N., Erdei L.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

2013

tom 94

nr 2

In vivo and in situ visualization of early physiological events induced by heavy metals in pea root meristem

100%

Lehotai N., Peto A., Bajkan S., Erdei L., Tari I., Kolbert Z.

Acta Physiologiae Plantarum

2011

tom 33

nr 6

Heavy metals (HMs) are toxic pollutants, which can negatively affect the physiological processes of plants; moreover, HMs can be present in the food chain endangering people’s health. The aim of this study was to investigate the early physiological events during HM exposure in the root tips of the food plant Pisum sativum L. Ten-day-old pea plants were treated with 100 µM CdCl₂ or CuSO₄, in nutrient solution for 48 h. We studied the rapid formation of different reactive oxygen species (hydrogen peroxide H₂O₂ and superoxide radical O₂⁻) and reactive nitrogen species (nitric oxide NO and peroxynitrite ONOO⁻) together with membrane damage and cell death in the meristem cells of pea roots using in vivo and in situ microscopic methods. In our experimental system, copper and cadmium induced the formation of H₂O₂ and NO. Two hours of heavy metal treatments resulted in an increased O₂⁻ formation; however, later the level of this reactive molecule dramatically decreased. We found that high levels of NO were needed for ONOO⁻ production under HM exposure. A fast loss of membrane integrity and decreased cell viability were detected in root tips of copper-treated plants. The effects of cadmium seemed to be slower compared to copper, but this non-essential metal also caused cell death. We concluded that viability decreased when NO and H₂O₂ levels were simultaneously high in the same tissues. Using the NO scavenger it was also evidenced that NO generation is essential for cell death induction under copper or cadmium stress.

Ograniczanie wyników

1 Acta Physiologiae Plantarum

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

2 Erdei L.

2 Kolbert Z.

2 Lehotai N.

2 Peto A.

1 Bajkan S.

1 Feigl G.

1 Tari I.

1 Tugyi N.

1 2013

1 2011

Wyniki wyszukiwania

Cytokinin overproducing ipt-161 Arabidopsis shows altered NO generation and insensitivity to selenite

In vivo and in situ visualization of early physiological events induced by heavy metals in pea root meristem