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For the primary purpose of minimizing carbon dioxide emissions in a megalopolis, an optimization model that remarkably reduces carbon emissions for the megalopolis, which is based on the inexact chanceconstrained linear programming (ICCLP) method and incorporates interval linear programming (ILP), and chance constrained programming (CCP), has been constructed. The corresponding net emissions of carbon dioxide results in probability levels of default equalling pᵢ =0.01, 0.05, 0.1 are [1,383.379, 1,825.311]×10⁴, [1,357.728, 1,800.841]×10⁴, [1,338.671, 1,780.060]×10⁴ tons in the megalopolis in 2015. Besides, the areas of different types of carbon-sinkable land of various cities within planned regions are obtained. The volume of energy consumption of dominating energy consumption industries in planned regions equals [965.52, 1,136.79]×10⁴ tons, which is reduced by [14.97, 22.09]%, while the intensity of energy consumption is decreased by [18.00, 20.00]% compared with that in 2010. Meanwhile, the intensities of carbon emissions are reduced by 20.00%, 19.00%, and 18.08%, respectively, under the conditions of pᵢ =0.01, 0.05, 0.1. It meets the requirements that carbon intensity shall be cut down by 17.00% in 2015 compared with that in 2010, which was proposed by “The 12th Five-Year Initiative of Controlling Greenhouse Gas Emissions.” The annual average GDP growth rate is 12.20%, reaching 9.79×10¹¹ yuan in total, higher than the expected annual growth rate of 10% in accordance with the development objective of “12th Five-Year” plan.
A full-length cDNA, SmPR10-1, encoding a pathogenesis-related class 10 protein, was isolated from Salvia miltiorrhiza, a well-known Chinese herbal plant. From the coding region of nucleotide sequence, the amino acid sequence can be predicted which contained conserved domain (K–A–X–E–X–Y) in the C-terminal helix found in most members of the PR10 protein family. Phylogenetic tree analysis indicated that the SmPR10-1 protein showed a sequence similarity which was much higher to dicot proteins than to other PR10 proteins. Prokaryotic expression of SmPR10-1 protein in fusion with a His-tag produced a 22 kDa protein in E. coli BL21 (DE3), which exhibited ribonuclease activity in vitro. The purified protein was used to assay antifungal activity and the results showed SmPR10-1 only inhibited the growth of Phytophthora infestans. The RT-PCR results showed that the SmPR10-1 was expressed in high transcript level in leaves, stalks and low levels in roots. This protein seems to involve in the plant active defense response through activation of the methyl jasmonate signaling pathway rather than salicylic acid or abscisic acid pathway.
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