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Using bioalcohols as a renewable energy source to substitute a part of fossil energy traffic and increasing the sustainability of individual transportation are important objectives in several countries. The global share of Bioethanol used for transportation is continuously increasing. Ethanol is a biomass-based renewable fuel that can be produced by alcoholic fermentation of sugar beet, sugar can, corn, and wheat (bio-ethanol), although petro-ethanol also exists, i.e., ethanol produced from fossil fuels. In the present tests we performed repeated cold starts performed with all investigated fuels, in two temperature ranges approaching 0ºC and 20ºC and with online measurement of different legislated and non-legislated emission components. The investigated fuel contained ethanol (E), in the portions of 10% and 85% by volume. The investigated vehicle represented a newer state of technology and an emission level of Euro 5. The engine works with homogenous GDI concept and with 3-W-catalyst (3WC). Since there is a special concern about the particle emissions of gasoline cars with direct injection, the nanoparticle counts measurements were systematically performed. The non-legislated gaseous emissions were tested at the tailpipe with FTIR, this with special focus on NH₃, HCHO (formaldehyde), and MeCHO (acetaldehyde).
Raw or extruded peas (Pisum sativum, pink-flowered cv. Milwa) were used. Extrusion reduced the trypsin inhibitor activity (TIA) from 2.35 to 0.35 mg · g–1, but did not affect tannins content, which was about 1 g · kg–1. A growth trial was conducted on 200 Ross 308 female broilers from day 1 to 35 of age. There were 3 treatment groups from day 1 to 14 including control without peas, 10% raw (RP) and extruded (EP) pea. From day 15 birds were kept individually, in groups of 18, and fed diets containing 15%, 20%, 25%, or 30% RP and EP. Chickens fed EP ate more feed (P ≤ 0.05) but had similar body weight gains and worse feed conversion ratio (P ≤ 0.05) than chickens fed RP. The apparent ileal digestibility (AID) of dry matter was lower in chickens fed EP than RP (P ≤ 0.01), whereas AID of protein did not differ. The AID of starch was 0.835 in the control group, in chickens fed both RP and EP it was, on average, 2 percentage points higher (P ≤ 0.01). In birds fed RP the short-chain fatty acid concentration in ileal digesta was lower (P ≤ 0.05) whereas in caecal digesta higher (P ≤ 0.01) than in birds fed EP. The dietary treatments had no effect on jejunal mucosa morphology. Diets containing up to 30% of peas with a relatively low tannins content and moderate TIA value, can be fed from the third week of life as a partial substitute of soyabean meal in broiler diets. The effects of pea extrusion on ileal nutrient digestibility and activity of gut microbiota was too small to affect performance
Lectin extracts were isolated from pea (PSA) and soyabean (SBA) seeds. In agglutination tests with human erythrocytes, SBA lectins showed higher activity than PSA lectins. The highest haemagglutination activity was observed with blood type AB, 50% lower activity with types A and 0, and the lowest, with type B. The experiment was performed on 30 male Wistar rats fed ad libitum a control diet or the same diet supplemented with PSA or SBA at a rate of 15 mg/day/rat for 14 days. Supplementation with lectins had a positive effect on feed intake and body weight gain, which differed significantly in the PSA group and numerically in the SBA group. PSA decreased the amylase activity in pancreatic juice and significantly decreased plasma glucose concentrations. Ca, P, Na, K, and Cl levels in pancreatic juice were similar in all groups. In rats fed the diets with lectin extracts, total pancreatic trypsin activity was significantly lower than in the control group and numerically lower in pancreatic juice. The results indicate that the effect of agglutinins from pea and soyabean seeds on pancreatic enzyme activity does not correlate with their agglutination activity.
The effect of extrusion, applied in the production of cold-pressed rapeseed cake on its nutritional value for broilers was evaluated. Two experiments were performed on 55 female broiler chickens, kept individually and allocated to 2 groups of 14 birds (growth trial) or to 3 groups of 9 chickens (digestibility trial). Rapeseed cakes pressed once (RC) or extruded and pressed again (RCE) were mixed in a 6:4 proportion with a basal diet and used in a balance trial with 3-week-old chickens. Furthermore, they were included into wheat-soyabean meal diets at a level of 100 or 150 g/kg and fed during 1-3 and 4-6 weeks of age, respectively, in a growth trial. Due to extrusion and additional pressing, the amount of crude fat decreased from17.4% in RC to 10% in REC, fat digestibility increased from 83 to 91% (P<0.01), protein digestibility decreased from 79 to 76% (P<0.01), and the metabolizable energy value decreased from 13.8 to 11.4 MJ/kg DM (P<0.01). Broilers ate more of the REC diet compared with the RC diet and their final body weight gain was higher (2.61 vs 2.39 kg; P<0.05). This, as well as a lower relative mass of the liver (P<0.05) and kidneys (P<0.01) and a slightly lower mass of thyroids, indicates that extrusion resulted in partial inactivation of the antinutritional factors present in rape seeds.
The effects of genetically modified (GM) soyabean meal (SBM) and GM maize (MON 810) on the performance and immunological response of broiler chickens were studied. Eight diets were prepared based on conventional or GM SBM (GTS 40-3-2) combined with non-modified maize cvs. Clarica or PR39 F58 or their isogenic GM counterparts, cvs. Bacilla or PR39 F56. Diets were fed in mash form from day one of age to 192 Ross 308 broiler females, allocated to 8 groups, 24 birds per group. From day 8 to 28 of age, 18 chickens per group were kept in individual cages and performance parameters were measured. After slaughter the shares of T helper and T cytotoxic cells in the blood were determined in 8 birds per group. The feed conversion ratio (FCR) of birds fed GM SBM was better by 3.2% than in birds fed conventional SBM (P < 0.01). The performance of birds fed GM maize did not differ from those fed conventional maize, but body weight gain was lower and FCR worse by 3.3% in birds fed diets with Clarica/Bacilla compared with the PR 39 cultivar. The shares of circulating lymphocytes in their entire population averaged 10% to 17% and 6.5 to 12.2% (T helper and T cytotoxic, respectively) for all groups except the group fed conventional cv. Clarica maize and non-modified SBM (31% and 20.6%, respectively). It may be concluded that the use of GM GTS 40-3-2 soyabean meal and GM MON 810 maize in broiler diets did not negatively affect the feed efficiency and blood lymphocyte subpopulations of broilers.
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