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1

Formation of DNA adducts in liver of rats treated with 2-aminofluorene. Differences between total and matrix attached DNA fractions

100%
Widlak P., Rzeszowska-Wolny J.
Acta Biochimica Polonica
|
1990
|
tom 37
|
nr 1
2

Separation of the complementary strands of DNA by centrifugation in alkaline CsSO4 gradient containing Ag+ OR Hg2+

100%
Filipski J., Rzeszowska-Wolny J.
Acta Biochimica Polonica
|
1974
|
tom 21
|
nr 3
3

Ionizing radiation-induced changes of gene expression patterns in human melanoma cells

100%
Herok R., Rzeszowska-Wolny J.
Acta Biochimica Polonica
|
2003
|
tom 50
|
nr Supplement 1
4

Formation and removal of DNA adducts in liver of rats treated with hepatocarcinogens 2-aminofluorene or 2-acetylaminofluorene

100%
Widlak P., Rzeszowska-Wolny J.
Acta Biochimica Polonica
|
1991
|
tom 38
|
nr 1
5

Msp18, the repetitive sequence specifically interacting with nuclear matrix of rat testis cells

100%
Rzeszowska-Wolny J., Rogolinski J.
Acta Biochimica Polonica
|
1994
|
tom 41
|
nr 4
The nuclear matrix bound DNA fraction of rat testis showed enrichment in repetitive sequences found in the 450 bp band after gel electrophoresis of the Mspl digested rat DNA. DNA fragments isolated from this band were cloned. DNA of the clone pMspI8 showed homology to some representatives of rat LINE sequence family, and complcxcd in vitro more efficiently with testes nuclear matrix proteins than with yeast ARS1 sequence containing the matrix association region (MAR) or DNA from an other clone, MspI19. Western blot analysis showed that MspI8 sequence interacts with testes matrix protein of about 120 kDa.
6

Effects of the hepatocarcinogen 2-aminofluorene on RNA synthesis in rat liver

100%
Widlak P., Rzeszowska-Wolny J.
Acta Biochimica Polonica
|
1992
|
tom 39
|
nr 1
7

UV-photoproducts induced in kappa Ig MAR increase its affinity to nuclear matrices in vitro

100%
Widlak P., Rzeszowska-Wolny J.
Cellular and Molecular Biology Letters
|
1997
|
tom 02
|
nr 2
8

Damaged DNA-binding proteins: recognition of N-acetoxyacetylaminofluorene-induced DNA adducts

100%
Rzeszowska-Wolny J., Widlak P.
Acta Biochimica Polonica
|
1999
|
tom 46
|
nr 1
Proteins which bind to the DNA damaged by genotoxic agents can be detected in all living organisms. Damage-recognition proteins are thought to be generally involved in DNA repair mechanisms. On the other hand, the relevance to DNA repair of some other proteins which show elevated affinity to damaged DNA (e.g. HMG-box containing proteins or histone H1) has not been established. Using the electrophoretic mobility-shift assay we have investigated damage-recognition proteins in nuclei from rat hepatocytes. We detected two different protein complexes which preferentially bound the DNA damaged by N-acetoxy-acetylaminofluorene. One of them also recognized the DNA damaged by benzo(a)pyrene diol epoxide (yet with much lower efficiency). The proteins which bind to damaged DNA are permanently present in rat cells and their level does not change after treatment of animals with the carcinogens. Differences in the affinity of the detected damage-recognition proteins to DNA lesion evoked by either carcinogen did not correlate with more efficient removal from hepatic DNA of 2-acetylaminofluorene-induced adducts than benzo(a)pyrene-induced ones.
9

DNA and proteins of the nuclear matrix are the main targets of benzo[a]pyrene's action in rat hepatocytes

100%
Widlak P., Rzeszowska-Wolny J.
Acta Biochimica Polonica
|
1993
|
tom 40
|
nr 4
The binding of [14Clbenzo[a]pyrene (B[aJP) to DNA and proteins in total nuclei and subnuclear fractions of cultured rat hepatocytes was compared. The main targets of BlaJP were non-histone high molecular weight proteins of the nuclear matrix and DNA sequences attached to this structure. Following 24 h exposure to B[alP the amounts of adducts in the nuclear matrix DNA and proteins were twice as high as in total nuclei. After withdrawal of the carcinogen containing medium the level of B[a]P-induced adducts gradually decreased but always remained the highest in the nuclear matrix proteins. Removal of adducts from the nuclear matrix DNA was more efficient than from the other DNA fractions, and 72 h after exposure to the carcinogen the level of DNA adducts in this fraction was similar to that in total nuclei.
10

The detection and purification of a protein that recognizes a line 1 DNA repetitive sequence

81%
Lukyanov D., Rogolinski J., Podgornaya O., Rzeszowska-Wolny J.
Cellular and Molecular Biology Letters
|
2000
|
tom 05
|
nr 4
551-521
A protein which binds specifically to MspI8 (a 454 bp long repeated sequence highly homologous to the 5’ untranslated region (5'UTR) of the LINE 1 sequence) was found and identified in nuclear extracts of rat liver cells. This protein was detected using the electrophoretic mobility shift assay (EMSA) and was purified by Q-Sepharose and DNA affinity chromatography. Its molecular mass was estimated by SDS electrophoresis as 29 kDa. The possibility that this protein (p29) is the rat analogue of human L1PBP-A, specific for the human LINE sequence LPE1, is discussed.
11

Association of SV40 DNA sequences with nuclear matrix in SV40 transformed hamster fibroblasts

81%
Rzeszowska-Wolny J., Lanuszewska J., Chorazy M.
Acta Biochimica Polonica
|
1987
|
tom 34
|
nr 2
12

Interaction of DNA with nuclear skeleton in rat hepatocytes

81%
Widlak P., Rogolinski J., Rzeszowska-Wolny J.
Acta Biochimica Polonica
|
1990
|
tom 37
|
nr 1
13

Inhibition of poly[ADP-ribose] polymerase activity affects its subcellular localization and DNA strand break rejoining

81%
Ryabokon N. I., Cieslar-Pobuda A., Rzeszowska-Wolny J.
Acta Biochimica Polonica
|
2009
|
tom 56
|
nr 2
243-248
 Poly(ADP-ribose) polymerase (PARP) plays a crucial role in DNA repair. Modulation of its activity by stimulation or inhibition is considered as a potentially important strategy in clinical practice, especially to sensitize tumor cells to chemo- and radiotherapy through inhibition of DNA repair. Here we studied the effect of the three PARP inhibitors, 5-iodo-6-amino-benzopyrone (INH2BP), 1,5-isoquinolinediol (1,5-dihydroxyisoquinolinediol (1,5-IQD) and 8-hydroxy-2-methylquinazolin-4-[3H]one (NU1025), and for two of them the efficiency in slowing the rejoining of DNA strand breaks induced by H2O2 was compared. Inhibition of PARP changed its intranuclear localization markedly; cells exposed to the inhibitor NU1025 showed a significant tendency to accumulate PARP in large foci, whereas in untreated cells its distribution was more uniform. The speed and efficiency of rejoining of H2O2-induced DNA strand breaks were lower in cells incubated with a PARP inhibitor, and the kinetics of rejoining were modulated in a different manner by each inhibitor. At a concentration of 100 µM the efficiency of the inhibitors could be ranked in the order NU1025 > IQD > INH2BP. The two first compounds were able to decrease the overall PARP activity below the level detected in control cells, while INH2BP showed up to 40% PARP activity after exposure to H2O2.
14

Metrizamide gradient centrifugation of histone-DNA complexes

81%
Rzeszowska-Wolny J., Grobner S., Filipski J., Chorazy M.
Acta Biochimica Polonica
|
1982
|
tom 29
|
nr 3-4
15

The genome-scale cellular responses to ionizing radiation

81%
Rzeszowska-Wolny J., Polanska J., Herok R., Polanski A.
Acta Biochimica Polonica
|
2006
|
tom 53
|
nr Supplement 1
16

Identification of genes important in modulation of radiosensibility of human melonoma cells

81%
Herok R., Fujarewicz K., Widel M., Rzeszowska-Wolny J.
Acta Biochimica Polonica
|
2003
|
tom 50
|
nr Supplement 1
17

Calculation of reliable transcript levels of annotated genes on the basis of multiple probe-sets in Affymetrix microarrays

81%
Jaksik R., Polanska J., Herok R., Rzeszowska-Wolny J.
Acta Biochimica Polonica
|
2009
|
tom 56
|
nr 2
271-277
 Microarray methods have become a basic tool in studies of global gene expression and changes in transcript levels. Affymetrix microarrays from the HGU133 series contain multiple probe-sets complementary to the same gene (4742 genes are represented by more than one probe-set in a microarray HGU133A). Individual probe-sets annotated to the same gene often show different hybridization signals and even opposite trends, which may result from some of them matching transcripts of more than one gene and from the existence of different splice-variant transcripts. Existing methods that redefine probe-sets and develop custom probe-set definitions use mathematical tools such as Matlab or the R statistical environment with the Bioconductor package (Gentleman et al., 2004, Genome Biol. 5: 280) and thus are directed to researchers with a good knowledge of bioinformatics. We propose here a new approach based on the principle that a probe-set which hybridizes to more than one transcript can be recognized because it produces a signal significantly different from others assigned to the particular gene, allowing it to be detected as an outlier in the group and eliminated from subsequent analyses. A simple freeware application has been developed (available at http://www.bioinformatics.aei.polsl.pl) that detects and removes outlying probe-sets and calculates average signal values for individual genes using the latest annotation database provided by Affymetrix. We illustrate this procedure using microarray data from our experiments aiming to study changes of transcription profile induced by ionizing radiation in human cells.
18

Comparison of real-time PCR and microarray methods in the assessment of gene expression changes induced by ionizing radiation in human tumor cell lines

81%
Herok R., Pawelek K.-M., Rzeszowska-Wolny J.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr Supplement 4
19

DNA damage and repair in lymphocytes of normal individuals and cancer patients: studies by the comet assay and micronucleus tests

81%
Palyvoda O., Polanska J., Wygoda A., Rzeszowska-Wolny J.
Acta Biochimica Polonica
|
2003
|
tom 50
|
nr 1
A population study is reported in which the DNA damage induced by y-radiation (2 Gy) and the kinetics of the subsequent repair were estimated by the comet and micronucleus assays in isolated lymphocytes of 82 healthy donors and patients with head and neck cancer before radiotherapy. The parameters of background and radia­tion-induced DNA damage, rate of repair, and residual non-repaired damage were measured by comet assay, and the repair kinetics for every donor were com­puter-fitted to an exponential curve. The level of background DNA damage before ir­radiation measured by comet assay as well as the level of micronuclei were signifi­cantly higher in the head and neck cancer patient group than in the healthy donors, while the parameters of repair were widely scattered in both groups. Cancer patient group contained significantly more individuals, whose irradiated lymphocytes showed high DNA damage, low repair rate and high non-repaired DNA damage level. Lymphocytes of donors belonging to this subgroup showed significantly lower inhibi­tion of cell cycle after irradiation.
20

Sequences flanking rat hsp70.1 gene contain motifs specific for matrix attached regions and form complexes with nuclear matrix proteins in vitro

81%
Widlak P., Rogolinski J., Rzeszowska-Wolny J.
Cellular and Molecular Biology Letters
|
1997
|
tom 02
|
nr 2
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