In recent years, the need for food safety and animal welfare has stimulated the search for new feeding strategies based on the use of natural feed additives, capable of ensuring the health of animals and excellence in their production performance. This study evaluates the phytochemical composition, antioxidant activity, and anti-hemolytic effects of a new product, Lisosan Reduction, containing the grain lysate Lisosan G and extracts of Picrorhiza and Desmodium. The data obtained were compared with those related to Lisosan G alone. Phytochemical compounds are present in high quantities in both products. The flavonoids, in particular, are doubled in Lisosan Reduction compared to Lisosan G. Lisosan Reduction also showed a greater antioxidant capacity, determined by the ORAC test, compared to Lisosan G. The antioxidant activity of Lisosan G and Lisosan Reduction was also evaluated in an ex-vivo system of human and dog erythrocytes (CAA-RBC). The results of this test show a higher antioxidant effect of the mixture Lisosan Reduction, compared to the lysate alone, both in human and canine samples, with greater effectiveness in human erythrocytes. The anti-hemolytic effects of Lisosan G and Lisosan Reduction were also evaluated, and the results show a stronger effect of Lisosan Reduction, compared with Lisosan G, in both human and dog samples. The results of our study demonstrate that Lisosan G and, in particular, Lisosan Reduction contain several bioactive compounds and have a strong antioxidant activity, suggesting the use of these compounds as feed additives to improve animal health.
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