Wyniki wyszukiwania

1

Czy chromosomy plciowe sa nam potrzebne?

100%

Kaluzewski B.

Kosmos

|

1994

|

tom 43

|

nr 3-4

371-388

2

"Test potrójny" - badanie przesiewowe trisomii chromosomu 21 oraz otwartych wad ośrodkowego układu nerwowego płodu

63%

Ferenc M., Kaluzewski B.

Diagnostyka Laboratoryjna

|

2000

|

tom 36

|

nr 4

3

Chromosomal mosaicism on amniotic interphase nuclei detected by multiprobe FISH

51%

Constantinou M., Zajac E., Kaluzewski B.

Journal of Applied Genetics

|

2003

|

tom 44

|

nr 4

4

Molecular cytogenetic techniques in detecting subtle chromosomal imbalances

51%

Kaluzewski B., Constantinou M., Zajac E.

Journal of Applied Genetics

|

2003

|

tom 44

|

nr 4

5

Inv in a patient hypogonadotropic hypogonadism

45%

Helszer Z., Lach J., Nowacka J., Constantinou M., Kaluzewski B.

Journal of Applied Genetics

|

2003

|

tom 44

|

nr 2

6

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The quantitative PCR technique resolves ambiguities concerning a small rearrangement of human chromosome 6q12-13

45%

Nowacka J., Helszer Z., Walter Z., Plucienniczak A., Kaluzewski B.

Journal of Applied Genetics

|

2001

|

tom 42

|

nr 4

7

Adaptation of PCR technique for quantitative estimation of genetic material from different regions of chromosome 21 in cases of trisomy 21

45%

Nowacka J., Helszer Z., Walter Z., Plucienniczak A., Kaluzewski B.

Acta Biochimica Polonica

|

2004

|

tom 51

|

nr 4

Pre- and postnatal diagnosis of chromosomal aberrations is generally based on conventional cytogenetic analysis. In this paper, we have devised a quantitative polymerase chain reaction (Q-PCR) method to determine gene dose effects and applied it in cases of regular trisomy 21 as a model. The method is based on quantitative assessment of PCR products after using primers amplifying DNA fragments located in the pericentromeric, heterochromatic, euchromatic and telomeric regions of chromosome 21. A gene dose effect on the amount of PCR product in cases of trisomy 21 was confirmed. Moreover, a correlation between the amount of the PCR product of the examined sequences and their location in the chromosome was observed. The obtained results suggest that the Q-PCR technique can be applied in the diagnosis of aneuploidies.

8

Prenatal detection of maternal UPD15 in new case with i[15p] by Timing Replication Test [TRT] and methylation analysis

45%

Constantinou M., Kaluzewski B., Helszer Z., Zajac E., Nowacka J.

Journal of Applied Genetics

|

2003

|

tom 44

|

nr 2

9

P53 mutations in urinary bladder cancer patients from Central Poland

39%

Borkowska E., Binka-Kowalska A., Constantinou M., Nawrocka A., Matych J., Kaluzewski B.

Journal of Applied Genetics

|

2007

|

tom 48

|

nr 2

177-183

The present study aimed at detection of P53 gene mutations in cells of urinary bladder neoplasms, as the mutations may be regarded as an independent prognostic factor for progression and recurrence of tumours. In the study, 82 patients with clinically diagnosed urinary bladder tumour were included. The control was composed of DNA samples from urine and blood of 202 healthy patients. Exons 5-8 of the P53 gene were screened for mutations by using multitemperature single-strand conformational polymorphism (MSSCP) analysis. Samples with abnormal MSSCP patterns were subjected to direct sequencing. The frequency of mutations in exons 5-8 of the P53 gene in patients with bladder cancer was lower (3.3% in grade G1,24% in G2, and 39% in G3) than the data reported in the literature. We found a higher percentage of polymorphism at codon 213 of the P53 gene in bladder cancer patients (6%), compared with the values in the reference group (2.5%). These results were matched with those of the loss of heterozygosity (LOH) analysis. In conclusion, mutations were found mainly in more advanced histopathological and clinical stages of the disease and at the CIS stage (carcinoma in situ). It cannot be excluded that the observed polymorphism at codon 213 may be a predisposing factor for urinary bladder carcinoma development.

10

Molecular diagnostics of promyelocytic leukaemia

39%

Kocki J., Constantinou M., Cioch M., Lancut M., Kaluzewski B., Dmoszynska A., Wojcierowski J.

Journal of Applied Genetics

|

2003

|

tom 44

|

nr 4

11

Application of multiplex FISH, CGH and MSSCP techniques for cytogenetic and molecular analysis of transitional cell carcinoma [TCC] cells in voided urine specimens

33%

Constantinou M., Binka-Kowalska A., Borkowska E., Zajac E., Jalmuzna P., Matych J., Nawrocka A., Kaluzewski B.

Journal of Applied Genetics

|

2006

|

tom 47

|

nr 3

Multiplex FISH (UroVysion), Comparative Genomic Hybridization (CGH), and Multitemperature Single-Strand Conformation Polymorphism (MSSCP) were applied for non-invasive diagnosis and prognosis of bladder cancer. The UroVysion test was positive in 80% of patients with pTl and in 100% of patients with either pT2 or pT3 tumours. Tumours with pT3T4 stages were characterized by high numbers of chromosomal imbalances, detected by CGH. The mutation of the p53 gene was detected in 16% of patients, but only in those with pT2 or pT3 tumours.

12

Co-expression of Epstein-Barr virus latent membrane protein 1, BCL-2, BCL-XL, P21CIP1 and P53 genes in human lymhoepithelial carcinoma

33%

Kocki J., Jarosz B., Constantinou M., Korszen-Pilecka I., Pilecki M., Antosz H., Staroslawska E., Korobowicz E., Kaluzewski B., Wojcierowski J.

Bulletin of the Veterinary Institute in Puławy. Supplement

|

2002

13

The risk of neoplasm associated with dysgenetic testes in prepubertal and pubertal/adult patients

27%

Slowikowska-Hilczer J., Szarras-Czapnik M., Wolski J. K., Oszukowska E., Hilczer M., Jakubowski L., Walczak-Jedrzejowska R., Marchlewska K., Filipiak E., Kaluzewski B., Baka-Ostrowska M., Niedzielski J., Kula K.

Folia Histochemica et Cytobiologica

|

2015

|

tom 53

|

nr 3

Ograniczanie wyników

Czy chromosomy plciowe sa nam potrzebne?

"Test potrójny" - badanie przesiewowe trisomii chromosomu 21 oraz otwartych wad ośrodkowego układu nerwowego płodu

Chromosomal mosaicism on amniotic interphase nuclei detected by multiprobe FISH

Molecular cytogenetic techniques in detecting subtle chromosomal imbalances

Inv in a patient hypogonadotropic hypogonadism

The quantitative PCR technique resolves ambiguities concerning a small rearrangement of human chromosome 6q12-13

Adaptation of PCR technique for quantitative estimation of genetic material from different regions of chromosome 21 in cases of trisomy 21

Prenatal detection of maternal UPD15 in new case with i[15p] by Timing Replication Test [TRT] and methylation analysis

P53 mutations in urinary bladder cancer patients from Central Poland

Molecular diagnostics of promyelocytic leukaemia

Application of multiplex FISH, CGH and MSSCP techniques for cytogenetic and molecular analysis of transitional cell carcinoma [TCC] cells in voided urine specimens

Co-expression of Epstein-Barr virus latent membrane protein 1, BCL-2, BCL-XL, P21CIP1 and P53 genes in human lymhoepithelial carcinoma

The risk of neoplasm associated with dysgenetic testes in prepubertal and pubertal/adult patients