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Increased nitrogen (N) and water availability, resulting from global changes or ecosystem management, were predicted to promote plant productivity and change community composition through shifts in competition hierarchies. So far, however, it still remains unclear how competitive interactions respond to N and water additions, which will be important to understand how plant community composition changes. To test plant competition ability in different successional stages under N and water addition treatments, a pot experiment under field conditions was performed. Six dominant plant species, three early-successional species, Artemisia lavandulaefolia, Artemisia capillaris, and Pennisetum centrasiaticum versus three late-successional species, Stipa krylovii, Leymus chinensis, and Artemisia frigida, were grown in monocultures and in two-species mixtures under factorial combinations of N and water addition treatments. We found that (1) there were interactive effects of N addition, water addition and interspecific competition on plant biomass; (2) For a given species, competitive abilities were correlated with biomass difference of neighboring species; (3) N and water additions interactively increased competition intensity and shifted species competitive hierarchies; (4) Late- successional species had stronger competitive abilities in the N addition treatment, whereas early-successional species had stronger competitive abilities after water addition or N + water addition. Our results show that N and water additions increased the intensity and impact of interspecific competition on plant growth, which has great implications for community structures. Since interspecific differences in competitive abilities were not well explained by species biomass, species identity, such as plant functional traits, should be included to predict the impact of increased N and water availability on plant communities and ecosystem functions.
To assess the impact of human activity on the nitrogen (N) cycle and evaluate the sources of N in surface water, the N budget for Laoguanhe River Watershed (LRW), a typical agricultural watershed and one of the upstream tributaries of Danjiangkou Reservoir in China, was developed by using measurement data on N fluxes and literature data on other parameters. Over the whole watershed, fertilizers, human and livestock excreta, atmospheric deposition, biotic fixation, N from crop residue used as fertilizer, and imported animal feeds contributed 65.7%, 20.7%, 6.1%, 5.6%, 1.7%, and 0.3%, respectively, to total N input (40,816.6 kg N km⁻² year⁻¹). N transported to water bodies, denitrification, harvesting crops, and ammonia volatilization contributed 32.0%, 25.2%, 23.0%, and 19.8% of total N output (21291.2 kg N km⁻² year⁻¹), respectively. The N budget for the LRW suggested that more than 50% of the N input was lost to the environment, and about 17% was discharged as riverine N, which indicated that agricultural and human activities in the watershed substantially impacted water quality, and so altered the N biogeochemistry process.
The dynamic strength and accumulated plastic strain are two important parameters for evaluating the dynamic response of soil. As a special clay, the remolded red clay is often used as the high speed railway subgrade filling, but studies on its dynamic characteristics are few. For a thorough analysis of the suitability of the remolded red clay as the subgrade filling, a series of long-term cyclic load triaxial test under different load histories are carried out. Considering the influence of compactness, confining pressure, consolidation ratio, vibration frequency and dynamic load to the remolded red clay dynamic property, the tests obtain the development curves of the dynamic strength and accumulated plastic strain under different test conditions. Then, through curve fitting method, two different hyperbolic models respectively for the dynamic strength and accumulated plastic strain are built, which can match the test datum well. By applying the dynamic strength model, the critical dynamic strength of the remolded red clay are gained. Meanwhile, for providing basic datum and reference for relevant projects, all key parameters for the dynamic strength and accumulated plastic strain of the remolded red clay are given in the paper
The purpose of this study was to investigate the protective effects of hydrogen reducing ischemia-reperfusion injury during CO2 pneumoperitoneum on oxidative stress and liver function. Eighteen healthy Beagle dogs were divided into three groups. Dogs in the control group were subjected only to anesthesia for 90 min. The pneumoperitoneum group was subjected to the pressure of CO2 pneumoperitoneum with 12 mmHg intraabdominal pressure for 90 min. The hydrogen group was subjected to the pressure of CO2 pneumoperitoneum with 12 mmHg intra-abdominal pressure for 90 min after a subcutaneous injection of hydrogen gas (0.2 mL/kg) for 10 min. Blood samples were collected before the induction of pneumoperitoneum, as well as 2 h and 6 h after deflation, to evaluate oxidative stress and liver function in serum. Liver tissue samples were taken 6 h after deflation for histopathological examination. In comparison with group P, a milder histopathological change was found in group H2, and the levels of hepatic function and anti-oxidation in group H2 were higher. Hydrogen gas reduced liver ischemia-reperfusion injury due to CO2 pneumoperitoneum by reducing oxidative stress and improving liver function. Hydrogen gas therapy can be considered as a way to reduce liver ischemiareperfusion injury in laparoscopic surgery.
Port shoreline resources are the basis of port and shipping development, and its assessment method has become one of the hot issues in port research. On the basis of constructing a reasonable index evaluation system, this paper constructs the fuzzy evaluation matrix based on the triangular fuzzy analytic hierarchy process and constructs the fuzzy evaluation matrix by using the fuzzy comprehensive evaluation method, and obtains the maximum membership degree of the port shoreline resources. Compared with the traditional port shoreline Resource evaluation methods, the new one got more advantages in objective and quantitative. Finally, Combined with the Nanjing section of the Yangtze River as a case for verification, the results show that the model can accurately solve the problem of resource evaluation of port shoreline
The Arabidopsis AtGRP7 gene, encoding a glycine-rich RNA-binding protein, has been shown to be involved in the regulation of a circadian-regulated negative feedback loop. However, little is known about the role of AtGRP7 in mediating abscisic acid (ABA) and stress responses. Here, we show that AtGRP7 plays a role in both. AtGRP7 was repressed by ABA, high salt and mannitol. Disruption of AtGRP7 by T-DNA insertion led to hypersensitive responses to ABA in both seed germination and root growth assays. The atgrp7-1 mutant was also hypersensitive to osmotic stress conditions, such as high salt and high concentrations of mannitol. In addition, the atgrp7-1 mutant plants accumulated significantly higher transcript levels of two ABA-and stress-inducible genes, RD29A and RAB18, compared with the wild-type plants. Taken together, these results suggest that AtGRP7 is involved in the regulation of ABA and stress responses.
MAX4 gene has been shown to be involved in the regulation of shoot branching in Arabidopsis (Arabidopsis thaliana). However, little is known about the role of MAX4 gene in low inorganic phosphate (Pi) stress response in Arabidopsis. Here we showed that MAX4 gene is involved in the regulation of low Pi stress response in Arabidopsis. MAX4 gene was repressed by low Pi stress, and the max4 mutants showed lower anthocyanin content and longer primary root length. In addition, max4 mutant plants also displayed altered root architecture such as increased root-to-shoot ratio, lower lateral root number and root hair density compared with wild-type plants under low Pi stress. Higher total Pi contents were detected in shoots and roots of max4 plants than those of wild-type plants when subjected to low Pi stress, which was associated, at least in part, with increase in expression of WRKY75 as well as AtPT1 and AtPT2 genes encoding high-affinity Pi transporters. Taken together, all these results suggest that MAX4 gene mediates low Pi stress response, at least in part, by regulating the expression of WRKY75 as well as AtPT1 and AtPT2 genes.
This study compounds three types of coagulants (AlCl₃, FeCl₃, Fe₂(SO₄)₃) with promising effects on TP removal of domestic sewage. The optimum conditions for TP removal using¹ compounded coagulants are determined by Plackett-Burman (P-B) design, steepest ascent, and Box-Behnken design. The adequacy of the quadratic regression model is evaluated by analysis of variance (ANOVA). Results show that initial pH, AlCl₃, and Fe₂(SO₄)₃ are the significant factors for TP removal. F-test, P-value (Prob>F), coefficients R², coefficient of variation, and adequate precision analysis demonstrated the goodness of fit for the regression model. The optimized conditions for TP removal determined by the response surface methodology are initial pH 5.2, AlCl₃ 45 mg/L, and Fe₂(SO₄)₃ 51 mg/L, respectively. The experimental TP removal efficiency (82.89%) agrees with the predicted response value (81.99%), thereby validating the feasibility of the model. Compared to single coagulants (AlCl₃, FeCl₃, Fe₂(SO₄)₃), the compounded coagulants demonstrate 3.29%, 7.59%, and 15.19% higher for TP removal; and 10.1%, 3.0%, and 10.3% higher for CODCr removal. In addition, the compounded coagulants also alleviate the potential hazards to human health due to the dosage decrease of aluminium salt coagulants.
Potassium (K+) is an essential element for plant growth and development. Under low-K+ stress, Arabidopsis (Arabidopsis thaliana) plants show K+-deficient symptoms, typically leaf chlorosis and subsequent inhibition of plant growth and development. The nonprotein amino acid b-amino-butyric acid (BABA) has been shown to have roles in protecting Arabidopsis against various pathogens as well as drought, high salinity, and cadmium stresses; However, little is known about the role of BABA in protecting Arabidopsis against low-K+ stress. Here, we showed that BABA protects Arabidopsis against low-K+ stress by increasing K+ uptake under low-K+ condition. Leaf chlorosis of plants subjected to low-K+ stress was abolished by BABA pretreatment, as indicated by a lower reduction in chlorophyll content in BABA-treated plants than watertreated plants. Low-K+ stress-induced decreases in both lateral root length and the numbers of lateral roots were improved by BABA pretreatment. In addition, under low-K+ stress, a significantly higher K+ concentration was detected in BABA-pretreated plants than in watertreated plants, and the transcript levels of AtHAK5 and LKS1 genes involved in K+ uptake in BABA-treated plants were higher than those of water-treated plants. Taken together, our results suggest that BABA plays a role in enhancing low-K+ stress tolerance by increasing K+ uptake, at least in part, via modulation of AtHAK5 and LKS1 under low-K+ condition.
Cytoplasmic male sterility (CMS) is a maternally inherited trait, which becomes an important way in heterosis breeding to simplify the seed production procedures and reduce the production cost. Cytological observation of pollen development stages showed a clear difference between the newly developed CMS line and its maintainer; the profiling comparisons between floral buds of CMS line and its maintainer were conducted using differential display reverse transcription PCR (DDRT-PCR) technology. Thirty genes were up-regulated and sixty genes were down-regulated in newly developed CMS line when compared with its maintainer. These genes were involved in cell wall biosynthesis and regulation, transporter and ion channel, flower development and protein metabolism, etc. Expression patterns of six genes encoding RsCAM6, RsGPI, RsPMEI, RsRac, RsCHS, and RsSTP9 were verified by RT-PCR and qRT-PCR in different development stage of floral buds and different organs of CMS line and its maintainer. The expression level of RsRac was higher in stamens and microspores of CMS line than in maintainer. In comparison, the expression levels of the other five genes in CMS line were lower compared with maintainer. This expression profile suggests that these genes played important roles in the development of the pollen and may be closely related to male sterility. The results observed in this study will contribute to understanding the mechanism of pollen abortion during CMS in radish.
It is generally assumed that plants can respond to varying degrees of physical damage by growth compensation via resprouting, and resprouting is a key functional trait in many species. Few studies have investigated how grass and shrub species distributed in moving dunes and semifixed dunes in semiarid areas respond to the combined effects of temperature and shoot removal. Medicago sativa, Artemisia ordosica, and Artemisia sphaerocephala plants were grown in a glasshouse for 8 weeks at air temperatures of 10/20°C, 12.5/22.5°C, 15/25°C, and 17.5/27.5°C (night/day) and were subjected to treatments of removing all leaves (LR), removing all leaves followed by cutting at half the plant height (HC), and removing all aboveground tissue (WC). The species, temperature, and damage extent had significant effects on the shoot number, leaf mass ratio, leaf area ratio and ratio of belowground to aboveground dry matter, and the species had a significant effect on the net assimilation rate, specific leaf area, and total biomass. The three species grew well under the HC and LR treatments, and high temperatures (15/25°C and 17.5/27.5°C) significantly promoted the regrowth of the three species. Medicago sativa grew faster than the two Artemisia species. Medicago sativa can be used for fertilizing or vegetation restoration in unimportant conservation areas, and the two Artemisia species can be effectively used for vegetation restoration in the Mu Us Sandland. Due to the low labor costs and the local climate conditions, plants should be clipped before the beginning of the main growing season (end of May or early June) to ensure rapid growth.
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