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Human adipose-derived stem cells for the treatment of intracerebral hemorrhage in rats via femoral intravenous injection

100%

Yang K. L., Lee J. T., Pang C. J., Lee T. Y., Chen S. P., Liew H. K., Chen S. Y., Chen T. Y., Lin P. Y.

Cellular and Molecular Biology Letters

2012

tom 17

nr 3

Human adipose-derived stem cells (huADSC) were generated from fat tissue of a 65-year-old male donor. Flow cytometry and reverse transcription polymerase chain reaction (RT-PCR) analyses indicated that the huADSC express neural cell proteins (MAP2, GFAP, nestin and β-III tubulin), neurotrophic growth factors (BDNF and GDNF), and the chemotactic factor CXCR4 and its corresponding ligand CXCL12. In addition, huADSC expressed the characteristic mesenchymal stem cell (MSC) markers CD29, CD44, CD73, CD90, CD105 and HLA class I. The huADSC were employed, via a right femoral vein injection, to treat rats inflicted with experimental intracerebral hemorrhage (ICH). Behavioral measurement on the experimental animals, seven days after the huADSC therapy, showed a significant functional improvement in the rats with stem cell therapy in comparison with rats of the control group without the stem cell therapy. The injected huADSC were detectable in the brains of the huADSC treated rats as determined by histochemistry analysis, suggesting a role of the infused huADSC in facilitating functional recovery of the experimental animals with ICH induced stroke.

Genetic polymorphisms of the DNA repair gene MPG may be associated with susceptibility to rheumatoid arthritis

81%

Chen S. Y., Wan L., Huang C. M., Huang Y. C., Sheu J. J. C., Lin Y. J., Liu S. P., Lan Y. C., Lai C. H., Lin C. W., Tsai C. H., Tsai F. J.

Journal of Applied Genetics

2010

tom 51

nr 4

Rheumatoid arthritis (RA) is a chronic autoimmune disease and can lead to deformities and severe disabilities, due to irreversible damage of tendons, joints, and bones. A previous study indicated that a DNA repair system was involved in the development of RA. In this study, we investigated the association of four N-methylpurine-DNA glycosylase (MPG) gene polymorphisms (rs3176364, rs710079, rs2858056, and rs2541632) with susceptibility to RA in 384 Taiwanese individuals (192 RA patients and 192 control subjects). Our data show a statistically significant difference in genotype frequency distributions at rs710079 and rs2858056 SNPs between RA patients and control groups (P = 0.040 and 0.029, respectively). Our data also indicated that individuals with the GG genotype at rs2858056 SNP may have a higher risk of developing RA. In addition, compared with the haplotype frequencies between case and control groups, individuals with the GCGC haplotype appeared to be at a greater risk of RA progression (P = 0.003, OR = 1.75; 95% CI = 1.20-1.55). Our results suggest that rs710079 and rs2858056 polymorphisms and the GCGC haplotype in the MPG gene are associated with the risk of RA progression, and thus may be used as molecular markers of RA if they are confirmed by further research.

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Journal of Applied Genetics

2 Chen S. Y.

1 Chen S. P.

1 Chen T. Y.

1 Huang C. M.

1 Huang Y. C.

1 Lai C. H.

1 Lan Y. C.

1 Lee J. T.

1 Lee T. Y.

1 Liew H. K.

1 Lin C. W.

1 Lin P. Y.

1 Lin Y. J.

1 Liu S. P.

1 Pang C. J.

1 Sheu J. J. C.

1 Tsai C. H.

1 Tsai F. J.

1 Wan L.

1 Yang K. L.

1 2012

1 2010

Wyniki wyszukiwania

Human adipose-derived stem cells for the treatment of intracerebral hemorrhage in rats via femoral intravenous injection

Genetic polymorphisms of the DNA repair gene MPG may be associated with susceptibility to rheumatoid arthritis