The aim of the study was to isolate and identify staphylococci from the intestinal samples of 24 trouts from East Slovakian waters. Moreover, their relation to antimicrobials was tested. The count of staphylococci in the trouts reached in average 4.0 × 10¹ colony forming units per gram. Twenty-two strains were identified by validated species-specific oligonucleotide array targeting the manganese-dependent superoxide dismutase-sodA gene. The identified strains were allotted to five species (Staphylococcus warnen, S. haemolyticus, S. epidermidis, S. hominis, S. pasteuri) clustered to three groups according to 16S rRNA sequences (S. epidermidis group, S. haemolyticus group, S. warneri group). These species belong to coagulase-negative staphylococci. All strains were sensitive to eight antibiotics out of 14 tested; the majority of strains were also sensitive to the remaining six antibiotics with the inhibitory zones from 13 to 41 mm. The strains were also sensitive at least to three enterocins of nine tested. Strains SW24/2, SHo 19/2, SHo20/l, SP19/1 were sensitive to eight of nine enterocins. All strains were sensitive to Ent A, P=EK13, and Ent EM41 with activity 100-6400 AU/mL. Strains SHo19/2 and SP19/1 were sensitive to Ent 2019 with activity up to 25600 AU/mL.
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