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The degraded ecosystem in the Loess Plateau region of China is very vulnerable, which has a great impact on regional sustainable development. Taking the Loess Plateau of Yan’an, Shaanxi province, China, as the study area, this study quantitatively evaluated conditions of the ecological environment. Based on natural factors, environmental factors, and socio-economic factors of Yan’an in 1997, 2004, and 2011, the dynamic changes in ecological vulnerability are analyzed with the application software for geographic information systems (GIS). The analytic hierarchy process (AHP) model was used and it contains variables for land use, soil erosion, topography, climate, vegetation, and socio-economic data. The evaluation results are divided into fi ve categories: potential, slight, light, medium, and heavy. The results show that the ecological vulnerability in the study is light level, and the ecological vulnerability index of the southern four counties is lower than that of the northern four counties. Ecological environment quality gradually improved in most areas in 1997~2011.
Safflower varieties were commonly divided into high, low and middle linoleic acid (LA) types according to their LA relative percentage contents in the seed oil. Fatty acid desaturase 2 (FAD2) plays a key role for LA content in seed. The sequence variations of FAD2 (CtFAD2-2, CtFAD2-10, CtFAD2-11) genes which could express in developmental seed of safflower were analyzed in 15 different LA-type materials. The results revealed that the CtFAD2-2 sequences were the same in all materials, and the CtFAD2-10, CtFAD2-11 sequences formed into two haplotypes independent of the LA-type of safflower seed. Yeast expression analysis revealed that two haplotypes of CtFAD2-10 had the function of oleic acid desaturase. The real-time PCR analysis of FAD2 in high and low LA-type materials at different seed developmental stages revealed that CtFAD2-2, CtFAD2-10 and CtFAD2-11 expressed mainly at 10 days after flower (DAF) for two different LA-type materials and the accumulation of few mRNA was detected in 14–22 DAF. The expression pattern of CtFAD2-1 in two different LA-type safflower materials was significantly divergent. For high-LA type, the accumulation of CtFAD2-1 mRNA was extremely low in 10 DAF, and its expression level increased sharply between 14 and 18 DAF and decreased slightly after 18 DAF. For low-LA type, the accumulation of CtFAD2-1 mRNA was extremely low during seed development stages. In conclusion, the gene structure and expression level of CtFAD2-1 may be the main factor affecting the differentiation of LA-type for safflower materials.
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