Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 12

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Plasma membrane Ca2plus -ATPase in excitable and nonexcitable cells

100%
Zylinska L., Soszynski M.
Acta Biochimica Polonica
|
2000
|
tom 47
|
nr 3
There is a significant number of data confirming that the maintenance of calcium homeostasis in a living cell is a complex, multiregulated process. Calcium efflux from excitable cells (i.e., neurons) occurs through two main systems an electrochemically driven Na+/Ca2+ exchanger with a low Ca2+ affinity (K0.5 = 10-15 µM), and a plasmalemmal, specific Ca2+-ATPase, with a high Ca2+ affinity (K0.5 <0.5-1 µM), whereas in nonexcitable cells (i.e., erythrocytes) the calcium pump is the sole system responsible for the extrusion of calcium ions. The plasma membrane Ca2+-ATPase (PMCA) is a ubiquitously expressed protein, and more than 26 transcripts of four PMCA genes are distributed in a tissue specific manner. Differences in the structure and localization of PMCA variants are thought to correlate with specific regulatory properties and may have consequences for proper cellular Ca2+ signaling. The regulatory mechanisms of calcium pump activity have been studied extensively, resulting in a new view of the functioning of this important molecule in the membranes.
2

Transport of 3-Bromopyruvate across the human erythrocyte membrane

81%
Sadowska-Bartosz I., Soszynski M., Bartosz G.
Current Topics in Biophysics. Supplement
|
2013
|
tom 36
|
nr 2A: Posters
3

Prooxidative effects of TEMPO in reactions with oxidants and glutathione

81%
Balcerczyk A., Soszynski M., Bartosz G.
Acta Biochimica Polonica
|
2003
|
tom 50
|
nr Supplement 1
4

Morphological and functional alterations of A549 cells after treatment with monochloramino acids and hypochlorite

81%
Robaszkiewicz A., Sicinska P., Bartosz G., Soszynski M.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr Supplement 4
5

Effect of amino acid peroxides on the erythrocyte membrane

81%
Soszynski M., Filipiak A., Bartosz G.
Current Topics in Biophysics
|
1994
|
tom 18
|
nr 2
6

Transport of 3 - bromopyruvate across the human erythrocyte membrane

71%
Sadowska-Bartosz I., Soszynski M., Ulaszewski S., Ko Y., Bartosz G.
Cellular and Molecular Biology Letters
|
2014
|
tom 19
|
nr 2
3-Bromopyruvic acid (3-BP) is a promising anticancer compound because it is a strong inhibitor of glycolytic enzymes, especially glyceraldehyde 3-phosphate dehydrogenase. The Warburg effect means that malignant cells are much more dependent on glycolysis than normal cells. Potential complications of anticancer therapy with 3-BP are side effects due to its interaction with normal cells, especially erythrocytes. Transport into cells is critical for 3-BP to have intracellular effects. The aim of our study was the kinetic characterization of 3-BP transport into human erythrocytes. 3-BP uptake by erythrocytes was linear within the first 3 min and pH-dependent. The transport rate decreased with increasing pH in the range of 6.0–8.0. The Km and Vm values for 3-BP transport were 0.89 mM and 0.94 mmol/(l cells x min), respectively. The transport was inhibited competitively by pyruvate and significantly inhibited by DIDS, SITS, and 1-cyano-4-hydroxycinnamic acid. Flavonoids also inhibited 3-BP transport: the most potent inhibition was found for luteolin and quercetin.
7

Peroxynitrite-induced hemolysis of human erythrocytes and its possible mechanism

71%
Soszynski M., Klimczak A., Grzelak A., Bartosz G., Zylinska L.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr Suppl.
8

Transport of organic anions by multidrug resistance-associated protein in the erythrocyte

71%
Rychlik B., Pulaski L., Sokal A., Soszynski M., Bartosz G.
Acta Biochimica Polonica
|
2000
|
tom 47
|
nr 3
The active transport of oxidized glutathione and glutathione S-conjugates has been demonstrated for the first time in erythrocytes and this cell remained the main subject of research on the glutathione S-conjugate pump for years. Further studies identified the glutathione S-conjugate pump as multidrug resistance-associated protein (MRP). Even though cells overexpressing MRP and isolated MRP provide useful information on MRP structure and function, the erythrocyte remains an interesting model cell for studies of MRP1 in its natural environment, including the substrate specificity and ATPase activity of the protein.
9

3-Bromopyruvic acid inhibits glucose transport

61%
Sadowska-Bartosz I., Zuberek M., Grzelak A., Sudak B., Soszynski M., Bartosz G.
Current Topics in Biophysics. Supplement
|
2016
|
tom 39
|
nr A: Plenary lectures
10

Hypochlorous acid inhibits glutathione S-conjugate transport

61%
Soszynski M., Zavodnik I., Zavodnik L. B., Zylinska L., Bartosz G., Bryszewska M.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr Suppl.
11

Antisense hairpin loop oligonucleotides as inhibitors of expression of multidrug resistance-associated protein 1: Their stability in fetal calf serum and human plasma

61%
Rebowski G., Wojcik M., Boczkowska M., Gendaszewska E., Soszynski M., Bartosz G., Niewiarowski W.
Acta Biochimica Polonica
|
2001
|
tom 48
|
nr 4
Multidrug resistance-associated protein (MRP1) is a transmembrane pump protein responsible for the efflux of chemotherapeutic drugs, an important cause of anticancer treatment failure. Trying to circumvent MRP-mediated resistance we de­signed and synthesized hairpin loops forming antisense oligodeoxyribonucleotides (ODNs), both phosphodiesters (PO-ODNs) and their phosphorothioate analogues (PS-ODNs), to reduce the protein expression by targeting its mRNA in a sequence spe­cific manner. Melting temperature measurements as well as polyacrylamide gel elec- trophoresis supported the preferential formation of a secondary structure, which was expected to protect ODNs against 3-exonuclease degradation. ODNs and PS-ODNs designed in this work were successfully tested as antisense inhibitors of the expres­sion of MRP1 in the leukaemia HL60/ADR cell line. Foreseeing the necessity to per­form clinical studies with such ODNs we investigated their stability against the 3 -exonuclease activity of fetal calf serum and human plasma. Under the conditions, corresponding to physiological ones, we observed high stability of hairpin loop form­ing ODNs, especially those containing longer (e.g. 7 base pair) stems. Comparative studies on the stability of chemically unmodified hairpin loop forming ODNs and their PS-counterparts indicated that endonuclease activity did not play any important role in the process of their nucleolytic degradation. Our studies provide strong evidence for high stability of chemically unmodified hairpin loop ODNs, making them an attractive alternative to phosphorothioate analogues commonly used in antisense strategy.
12

Multidrug resistance-associated protein - reduction of expression in human leukaemia cells by antisense phosphorothioate oligonucleotides

51%
Niewiarowski W., Gendaszewska E., Rebowski G., Wojcik M., Mikolajczyk B., Goos W., Soszynski M., Bartosz G.
Acta Biochimica Polonica
|
2000
|
tom 47
|
nr 4
Multidrug resistance-associated protein (MRP1) causes cellular drug resistance in several cancer cell lines. In this paper we show that antisense oligonucleotides decrease MRP1 expression in human leukaemia cells. We investigated biological activity of a series of 12 linear phosphorothioate oligonucleotides, complementary to several regions of MRP1 mRNA. The oligonucleotides were administered to leukaemia HL60/ADR cells overexpressing MRP1 protein. Then, the level of MRP1 mRNA was determined by means of semiquantitative RT-PCR and the protein level by reaction with specific monoclonal antibodies. Some of the investigated antisense oligonucleotides decrease the expression level of the MRP1 protein by 46% and its mRNA level by 76%.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.