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Beta-hydroxybutyrate in milk as screening test for subclinical ketosis in dairy cows

100%

Jezek J., Cincovic M. R., Nemec M., Belic B., Djokovic R., Klinkon M., Staric J.

Polish Journal of Veterinary Sciences

2017

tom 20

nr 3

Ketosis is a very frequent metabolic disease in dairy cows, resulting in lower milk production, impaired fertility and increased frequency of other diseases. The course of the disease is often subclinical, so early detection is very important. The aim of the study was to investigate the relation between the concentration of beta-hydroxybutyrate in blood and milk and to determine the cut-off value in milk for detection of subclinical ketosis. The study included 94 cows, which were in the first third of lactation. Beta-hydroxybutyrate (BHB) concentrations were measured in blood and milk serum using a biochemical analyser. The average concentration of BHB in the blood serum samples was 1.14 mmol/L while in the milk it was about ten times lower at 0.117 mmol/L. A statistically significant positive correlation between the concentration of BHB in blood and milk (r=0.705, p<0.001) was found. In cows with BHB in blood below 2.0 mmol/L a stronger correlation between blood and milk BHB was established (r=0.658, p<0.001) than in cows with blood BHB above 2.0 mmol/L (r=-0.292, p=0.206). Therefore, BHB in milk is a very suitable indicator in the diagnosis of subclinical ketosis as there is a good correlation between BHB in the blood and milk of cows with subclinical ketosis. The cut-off concentration of BHB in milk set at ≥0.080 mmol/L (AUC=0.91±0.03; p<0.001) is a significant indicator for subclinical ketosis in dairy cows. The sensitivity of the test was 94% and specificity 74%. Beta-hydroxybutyrate in milk is a good indicator of subclinical ketosis in dairy cows and can be measured accurately with a biochemical analyser.

Body condition score loss, hepatic lipidosis and selected blood metabolites in Holstein cows during transition period

100%

Samanc H., Gvozdic D., Fratric N., Kirovski D., Djokovic R., Sladojevic Z., Cincovic M.

Animal Science Papers and Reports

2015

tom 33

nr 1

The aim of this study was to investigate the relationship between the loss in body condition score (BCS) and loss (ΔBCS), energy balance (EB), hepatic lipidosis and blood serum concentration of non esterified fatty acids (NEFA), glucose, triacylglycerol (TAG) and total bilirubin (tBIL) in healthy dairy cows during transition period. Twenty healthy Holstein cows were included and categorized into groups based on BCS loss (ΔBCS) between dry period and early lactation (ΔBCS <0.75 and ≥0.75). Significant differences between groups (p<0.05) were observed for blood serum NEFA, glucose and tBIL. Cows with high ΔBCS (≥0.75) between dry period and early lactation showed increased blood serum NEFA, TAG and tBIL concentrations and lower blood serum glucose concentration during transition period, compared to the low ΔBCS cows (<0.75). Metabolic profiles of cows during transition period indicate high level of lipid mobilization from adipose tissue, possible decreased liver cells TAG export and gluconeogenic ability and impaired bilirubin metabolism if ΔBCS is increased by more than 0.75 points. ΔBCS was in relation with energy balance (EB) in transition period.

Evaluation of the metabolic status of Simmental dairy cows in early and mid lactation

84%

Djokovic R., Kurcubic V., Ilic Z., Cincovic M., Fratric N., Stanimirovic Z., Petrovic M. D., Petrovic M. P.

Animal Science Papers and Reports

2013

tom 31

nr 2

Fifteen early-lactation cows and 15 mid-lactation cows were chosen for the analysis. Blood samples were collected to measure the beta-hydroxybutyrate (BHB), non-esterified fatty acids (NEFA), triglycerides (TG), glucose (Glu), total protein (TP), albumin (ALB), total bilirubin (TB), urea (U)and the activity of aspartate transaminase (AST) and gamma-glutamyl transferase (GGT). Earlylactation cows showed significantly higher (P<0.05) values of serum BHB and NEFA, and lower of (P<0.05) glycemia levels compared to mid-lactation cows. High lipomobilization (NEFA >0.4 mmol/l) and subclinical ketosis (BHB >1.2 mmol/L) were detected in 6 (40%) and 14 (94.4%) early-lactation cows, respectively, and in none of the mid lactation cows. AST activities above 100 IU/l were detected in two early-lactation and none of the mid-lactation cows. TG concentrations below 0.12 mmol/l were found in 7 (44%) early-lactation and 2 (13.3%) mid-lactation cows. Glucose levels were below 2.5 mmol/l in 10 (66.6%) early-lactation and 5 (33.3%) mid-lactation cows. Early-lactation cows showed lower blood serum concentrations of TG (P>0.05), ALB (P>0.05), TP (P<0.05), U (P>0.05) and GTT (P>0.05) activities and higher concentrations of TB (P>0.05) and AST activities (P<0.05),as compared to mid-lactation cows. These metabolic characteristics were correlated with DMI and energy balance (EB). Blood serum values for glucose, TG, BHB, NEFA and AST showed that earlylactation cows suffered from metabolic disturbances, associated with ketosis, and some degree of hepatic lesions, probably due to fat infiltration. These serum parameters may have a key role in evaluating the metabolic status of dairy cows.

Ograniczanie wyników

2 Animal Science Papers and Reports

1 Polish Journal of Veterinary Sciences

3 Djokovic R.

2 Cincovic M.

2 Fratric N.

1 Belic B.

1 Cincovic M. R.

1 Gvozdic D.

1 Ilic Z.

1 Jezek J.

1 Kirovski D.

1 Klinkon M.

1 Kurcubic V.

1 Nemec M.

1 Petrovic M. D.

1 Petrovic M. P.

1 Samanc H.

1 Sladojevic Z.

1 Stanimirovic Z.

1 Staric J.

1 2017

1 2015

1 2013

Wyniki wyszukiwania

Beta-hydroxybutyrate in milk as screening test for subclinical ketosis in dairy cows

Body condition score loss, hepatic lipidosis and selected blood metabolites in Holstein cows during transition period

Evaluation of the metabolic status of Simmental dairy cows in early and mid lactation