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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Hookworm infections in human and laboratory animals - differences and similarities in immune responses

100%
Dlugosz E.
Wiadomości Parazytologiczne
|
2005
|
tom 51
|
nr 3
2
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Content available

Wykorzystanie sekwencji genu rRNA w molekularnej diagnostyce parazytologicznej

63%
Dlugosz E., Wisniewski M.
Wiadomości Parazytologiczne
|
2006
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tom 52
|
nr 4
263-269
Ribosomal RNA (rRNA) is a component of the ribosomes. Eukaryotic ribosomes contain four different rRNA molecules: 18S, 5,8S, 28S and 5S rRNA. rRNA is the most conserved (least variable) gene in all cells. For this reason, genes that encode the rRNA (rDNA) are sequenced to identify an organism's taxonomic group, calculate related groups, and estimate rates of species divergence. Especially the internal transcribed spacers (ITS) are very useful for molecular diagnostic of parasite. They are noncoding regions of DNA sequence that separate genes coding for the 28S, 5.8S, and 18S ribosomal RNAs. These ribosomal RNA (rRNA) genes are highly conserved across taxa while the spacers between them may be species-specific. In this paper authors describe practical using of rRNA gene to parasite diagnostic.
3

Toxocara canis glycans influence antigen recognition by mouse IgG1 and IgM antibodies

63%
Dlugosz E., Wisniewski M.
Acta Parasitologica
|
2016
|
tom 61
|
nr 1
4

Rodent interleukins - their molecular characteristics and a role in immunological responses against parasitic infections

63%
Dlugosz E., Wedrychowicz H.
Acta Parasitologica
|
2004
|
tom 49
|
nr 3
Interleukins play a very important regulatory role in the immunological system. Much attention is payed to understand their complicated activities during parasite infections. To investigate their presence in host organisms techniques of molecular biology have been applied. Nucleotide sequences of many interleukins have been established and analysed. The coding sequences of different species are highly conserved. Rodents are especially investigated because of their usefulness as laboratory models for many animal and human parasitic diseases. Here we take a look at mouse (Mus musculus), rat (Rattus norvegicus), hamster (Mesocricetus auratus) and guinea pig (Cavia porcellus) interleukins and compare their coding sequences and gene structures.
5

Filariozy - zagrożenia zdrowia na świecie i w Polsce

51%
Dobrzynski A., Klockiewicz M., Dlugosz E.
Magazyn Weterynaryjny
|
2017
|
tom 26
|
nr 03
6

Wstępne rozpoznanie epidemiologiczne inwazji pasożytów jelitowych w wybranych fermach nerek w Polsce

51%
Klockiewicz M., Jakubowski T., Janecka E., Dlugosz E.
Medycyna Weterynaryjna
|
2013
|
tom 69
|
nr 07
The aim of the study was to estimate the prevalence of intestinal parasitic infections in mink. A preliminary investigation was conducted on nine Polish mink farms (approx. 510,000 animals surveyed). One hundred and five aggregated samples of mink feces were randomly collected and examined by flotation coproscopy and McMaster’s method. Although coccidia oocysts (of unrecognized genera) were detected in samples from all the farms, the intensity of infections was low (OPG from 0 to 5500). Data concerning the prevalence of intestinal parasitic infections in farm mink in Poland may constitute an important contribution to a research-based explanation of the actual role of mink in the transmission of parasitic zoonoses. It is worth noting that although coprological investigations showed a widespread prevalence of coccidian infections in these animals, no developmental forms of other intestinal parasites, such as the eggs of roundworms occurring in carnivores, were found. These results suggest that adequately prepared (e.g. composted) mink manure can be used instead of chemical fertilizers as a valuable natural fertilizer for arable crops without creating a hazard for humans.
7

Histopathological lesions caused by experimental Toxocara canis and Toxascaris leonina infections in farm mink (Neovison vison)

51%
Klockiewicz M., Sobczak-Filipiak M., Jakubowski T., Dlugosz E.
Journal of Veterinary Research
|
2019
|
tom 63
|
nr 2
8

Liver enzyme activity in dogs infected with Babesia canis

51%
Zygner W., Gojska-Zygner O., Dlugosz E., Wedrychowicz H.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 3
The influence of anaemia on alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) activities in dogs infected with B. canis was investigated. Samples of blood and serum from 230 infected dogs were divided into two groups: A (with anaemia) and B (without anaemia). The differences in the activities of the enzymes between both groups were not statistically significant. These results suggest that mild anaemia, as the only factor, has no influence on ALT, AST, or ALP activity in canine babesiosis. However, this study certainly cannot exclude the possibility that a more severe anaemia can have a major effect on the liver.
9

In vitro IL-4, IL-12, and IFN-gamma production by splenocytes from Ancylostoma ceylanicum infected hamsters

51%
Dlugosz E., Wisniewski M., Wojcik L., Wedrychowicz H.
Bulletin of the Veterinary Institute in Puławy
|
2010
|
tom 54
|
nr 3
The aim of the study was the production of chicken polyclonal antibodies recognising hamster IL-4, IL-12p35, and IFN-γ, which were further used to estimate cytokine concentrations in splenocyte culture supernatants, obtained from three age groups of Ancylostoma ceylanicum infected hamsters. Worm burdens differed significantly among age groups; the younger animals were the more heavily infected. Th1 and Th2 type cytokines were detected only in cultures containing spleen cells from the oldest hamsters, which were the most resistant to hookworm infection. In cultures of cells from young more susceptible hamsters, the presence of IL-4 and IL-12 but not IFN-γ were observed.
10
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Co-existance of toxoplasmosis and neuroborreliosis - a case report

51%
Gustaw K., Beltowska K., Dlugosz E.
Annals of Agricultural and Environmental Medicine
|
2005
|
tom 12
|
nr 2
305-308
The 53-year-old woman was initially diagnosed with multiple sclerosis, despite the fact that she did not really meet the clinical criteria. Her only symptoms were clumsiness and weakness of the right extremities. Being a veterinary research worker she had been exposed to infectious material. In 1995, she was diagnosed with ELISA as having toxoplasmosis and treated as such. In 2002, after the infectious, flu-like disease, she revealed arthritis and drowsiness, also with memory and language impairment. The patient continued to have symptoms consistent with previously examined clumsiness. She was diagnosed with Lyme via ELISA and PCR, and treated. She made a full recovery from acute symptoms. After a few months, neurological and neuropsychological examinations were performed. On the background of mild cognitive decline apraxia and difficulties of attention were noted as the main problems. A apraxia of the right hand complicated the patient's life and depreciated her quality of life. The patient underwent MRI examination. FSE, FAST and FLAIR sequences were made. The MRI demonstrated the appearance of several small hyperintense lesions in the white matter of the left and right frontal and left parietal lobe. These lesions were typical of the post-inflammatory leucoencephalopathy. Additionally, a ring-shaped, low-intensity lesion in the posterior part of the left parietal lobe was noticed. The lesion was 8 mm in diameter and described to be an old toxoplasmosis lesion. The patient had been treated and the symptoms consistent with Lyme disease resolved. Patient continues to have symptoms consistent with focal destruction of the parietal lobe. Over the past six months, she has not progressed and relapsed in a manner that is consistent with MS.
11
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Poszukiwanie antygenow wzbudzajacych odpornosc przeciwko inwazjom tegoryjcow

51%
Wisniewski M., Dlugosz E., Baska P., Wedrychowicz H.
Wiadomości Parazytologiczne
|
2006
|
tom 52
|
nr 4
271-276
Hookworms are very important blood sucking nematode parasites of humans and domestic animals. The host with a heavy infection can lose almost a cup of blood per day. This may contribute to anemia which is associated with many physical and mental developmental insults. The works on obtaining an effective hookworm vaccine have been lasting for about eighty years. Recent identifications of a number of bioactive molecules produced by larval and adult stages of Ancylostomatidae are very helpful for selecting of nematode proteins crucial for host-parasite interactions and promising vaccine antigens. Many of these molecules are involved in host skin penetration by infective larvae, intestinal tissue invasion and digestion of haemoglobin and/or other macromolecular substrates. However, the results of many vaccination trials using recombinant forms of these proteins showed no sufficient protection against experimental hookworm infections.
12

Inwazje pasożytnicze u kociąt i szczeniąt - jakie są rzeczywiste zagrożenia?

51%
Klockiewicz M., Dlugosz E., Dobrzynski A.
Magazyn Weterynaryjny. Zeszyt Edukacyjny
|
2017
|
nr 6. Parazytologia psów i kotów
13
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

A review of the occurrence and clinical consequences of protozoan infections in carnivorous fur farm animals

51%
Klockiewicz M., Dlugosz E., Jakubowski T.
Annals of Agricultural and Environmental Medicine
|
2021
|
tom 28
|
nr 2
14

Biochemical abnormalities observed in serum of dogs infected with large Babesia in Warsaw (Poland)

45%
Zygner W., Rapacka G., Gojska-Zygner O., Dlugosz E., Wedrychowicz H.
Polish Journal of Veterinary Sciences
|
2007
|
tom 10
|
nr 4
Biochemical abnormalities observed in canine babesiosis are related to the severity of the disease. The primary biochemical abnormalities found in affected dogs are: increase of the serum activity of transaminases and alkaline phosphatase, azotemia, and hypoglycemia. The purposes of this study were: 1) to estimate biochemical abnormalities in dogs infected with large Babesia in Warsaw and 2) to evaluate statistically changes observed during canine babesiosis in dogs from Warsaw. Samples of serum were collected from dogs naturally infected with large Babesia. Among 2023 positive samples, 202 were randomly selected. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), creatinine, blood urea nitrogen (BUN), total serum protein (TSP), albumin and blood glucose concentration were determined with a clinical chemistry analyser. Elevated activity of ALT, AST and ALP was detected accordingly in: 64.9, 92.6 and 31.7% of dogs. Elevated creatinine concentration and BUN were detected accordingly in 30.7 and 62.4% of dogs. Decrease of TSP, albumin, BUN, and hypoglycemia was detected accordingly in: 19.8, 32.7, 1.5 and 18.3% of dogs. The most common biochemical abnormalities found in affected dogs were: increase of activity of transaminases and ALP, elevated creatinine concentration, hypoalbuminemia and hypoglycemia. These abnormalities resulted from hepatopathy, renal failure and fasting.
15

Experimental infection with T. canis and T. leonina in farm mink (Neovison vison)

45%
Klockiewicz M., Jakubowski T., Sobczak-Filipiak M., Bartosik J., Dlugosz E.
Journal of Veterinary Research
|
2019
|
tom 63
|
nr 2
16

Przypadek wertykalnej inwazji Dirofilaria repens w rodzinie kotów

45%
Klockiewicz M., Szmidt A., Dobrzynski A., Wysmolek M., Dlugosz E.
Magazyn Weterynaryjny
|
2018
|
tom Wydanie Specjalne Nr 1 marzec
17

Production of TGF-beta1 specific chicken polyclonal antibodies and their use in analysis of cytokine level in hookworm infected hamsters

45%
Dlugosz E., Lenard A., Baska P., Wedrychowicz H., Wisniewski M.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 4
In vitro production of a potent immunoregulatory cytokine - TGF-β1 in mesenteric lymph node (MLN) cell cultures derived from three age groups of Syrian golden hamsters infected with Ancylostoma ceylanicum was investigated. Expression of TGF-β1 mRNA in the cells was measured by real-time quantitative PCR. Protein concentration in cell culture supernatants was determined by ELISA with the use of generated chicken polyclonal antibodies recognising hamster TGF-β1. The highest concentration of this cytokine was released by cells from the youngest hamsters, which were the most susceptible to hookworm infection. Real-time PCR analysis of TGF-β1 mRNA expression in non-stimulated MLN cells showed that fold changes in mRNA expression in the age groups corresponded to protein concentration in culture supernatants. To our knowledge this is the first report of TGF-β1 production during hookworm infection in hamsters. We hope that our studies provide a basic start for further investigation of TGF-β1 role in immunosuppression during hookworm infection.
18
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Molecular identification of tapeworms from Konik Polski horses from Biebrza National Park

45%
Bartosik J., Lojek J., Dlugosz E., Gorski P., Zygner W.
Annals of Parasitology
|
2021
|
tom 67
|
nr 4
19

Vaccination with a cocktail of Ancylostoma ceylanicum recombinant antigens leads to worm burden reduction in hamsters

39%
Wisniewski M., Lapinski M., Danilowicz-Luebert E., Jaros S., Dlugosz E., Wedrychowicz H.
Acta Parasitologica
|
2016
|
tom 61
|
nr 3
20

Analysis of IL-2 production in hookworm infected hamsters using generated polyclonal antibodies

39%
Dlugosz E., Cendrowski J., Baska P., Siwinska A., Wedrychowicz H., Wisniewski M.
Bulletin of the Veterinary Institute in Puławy
|
2012
|
tom 56
|
nr 1
The aim of the study was cloning and anahsis of the entire coding sequence of hamster IL-2 by the method of RACE-PCR, its expression in Escherichia coli cells, and production of IL-2 specific antibodies. These antibodies were used to determine in vitro IL-2 production by cells derived from the spleen and mesenteric lymph nodes of Ancylostoma ceylanicum infected hamsters. The highest concentration of IL-2 was noted in supernatants from cell cultures coming from the oldest, most resistant hamsters.
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