Fusarium oxysporum f. sp. psidii and F. solani, causal agents of wilt in guava are highly variable pathogens. This study was conducted on cultural and physiological (temperature and pH) characters. The data revealed that maximum mycelial growth was obtained in potato dextrose agar as semi-solid media i.e. 78.00 mm for F. oxysporum f. sp. psidii; 73. 83 mm for F. solani, while malt extract broth as liquid broth media i.e. 1 385 mg mycelia for F. oxysporum f. sp. psidii; 1491 mg for F. solani. Maximum sporulation was recorded in oatmeal agar and mycological broth. The optimum temperature and pH for growth of both Fusarium spp. isolates was 28°C and 5.5. The isolates differed in their colony growth; mycelial mass, macro-conidia, and micro-conidia produced. These variations were characters of each of the isolates with respect to cultural and physiological characters.
The paper concerns randomly amplified polymorphic DNA RAPD-PCR analysis of seven Trichoderma speciesisolates and their in vitro antagonism against wilt pathogens of Psidium guajava L. viz. Fusarium oxysporum f. sp. psidii (F. o.f. sp. psidii) and Fusarium solani. Out of 10 RAPD oligonucleotides (OPA 1-OPA 10) tested, seven markers OPA 1, 3, 5, 7, 8, 9 and10 efficiently differentiated the isolates of Trichoderma and showed reproducible banding patterns. A total of 248 bands were obtained from these markers along with a 61.84% per cent similarity among the seven isolates of Trichoderma.From the seven isolates of Trichoderma, isolates T. harzianum, T.virens and T. viride were evaluated for in vitro efficacy against F. o. f. sp. psidii and F. solani. Per cent inhibition was maximum by direct use of Trichoderma spp. in dual cultures against both pathogens, F. o. f. sp. psidii and F.solani. Two species, T. virens and T. viride were superior in inhibiting the growth of both Fusarium spp. Fusarium isolates showed intra species variability.
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