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The aim of this study was to explore ecological adaptation of seed microsculptures of Saussurea from different altitudes. Scanning electron microscopy was used to observe the cypselae surface of 10 taxa of Saussurea collected from the eastern edge of Qinghai-Tibet plateau, China. The results indicated that the microsculptures had clear and consistent variations in some samples collected from high (4200 m a.s.l.) and low-altitude sites (2200 m a.s.l.). The thickness of stripes and distance between stripes are increasing while sulcus depth is increasing from a low to a high altitude (P < 0.01). The seed-coat surface was becoming rougher from low to high altitude. The surviving rate of seeds was lower at high altitude than that at low altitude in all the species. The surviving rate of the seeds collected from the high altitudes was higher than that of the seeds collected from low altitudes when sown at the same altitude. All the results suggested that there is selection pressure of the altitude on the development of the microsculpture patterns, which increases absorbed sunlight, the residence time of soil water and nutrition. The changes can make the seeds survive better at extreme environments (cold and dry). The cypselae microsculpture patterns should be used as potential adaptation biomarkers for the species of Saussurea from low- to high-altitude.
Gibberellic acid (GA₃) is a plant growth regulator commonly used for increasing the productivity of seedless fruit. However, little is known about the effect of GA₃ on phenolic compounds in grapevine. In the present study, the effect of GA₃ application on contents and antioxidant activities of phenolic compounds in different tissues of Muscat grapevine (Vitis vinifera L. cv. Muscat) was investigated. An application of 100 mg/L GA₃ could successfully induce seedless grape berry, enhance berry size and accelerate the development of berries, resulting in earlier ripening of seedless berry. The contents of total phenolics and total flavonoids, and antioxidant activities in leaf, stem and tendril obviously increased, while these remarkably decreased in berry skin and flesh after GA₃ application. In addition, the grapevine leaf, stem, tendril and skin extracts were shown to contain high amounts of phenolics and significant antioxidant activities. Thus, these findings indicate that GA₃ application causes different effect on phenolic compounds and antioxidant activities, depending on grapevine tissues.
This research was undertaken in order to determine and analyze various heavy metals present in sediments taken from Lake Donghu. Six heavy metals: arsenic (As), cadmium (Cd), chromium (Cr), copper (Cu), lead (Pb), and zinc (Zn) were determined by inductively coupled plasma-atomic emission spectroscopy (ICPAES). Relative enrichment factors and potential ecological risk indexes were used to study the pollution status of heavy metals in sediments and assess their potential ecological risk to the environment. Sediment enrichment factors of heavy metals were found in the following order Cr > As > Cd > Zn > Cu > Pb. Potential ecological risk indices for heavy metals were: Cd > As > Cr > Cu > Pb > Zn. The calculated potential ecological risk indices showed that the lake was polluted by heavy metals. Cd had moderate potential ecological risk to the ecological environment and was the main contributor to potential toxicity response indices for various heavy metals in Lake Donghu. Analysis of variance (ANOVA) was used to establish the significance of variations in heavy metals. The mean seasonal concentrations of metals showed that there were significant differences among seasons and sampling sites (P<0.05). The cause of pollution in Lake Donghu could be associated with industrial and human activities. We proposed strategies that can be applied in order to prevent accumulation of heavy metals in the lake.
This study investigated the role of autophagy in the survival of the invasive bacterium Brucella melitensis strain 16M in murine macrophages. Here, Brucella melitensis 16M was found to trigger autophagosome formation, enhance autophagy flux and increase the expression level of the autophagy marker protein LC3-II. When autophagy was pharmacologically inhibited by 3-methyladenine (3-MA), Brucella replication efficiency was significantly decreased (p < 0.05). These results suggest that autophagy favors Brucella melitensis 16M survival in murine macrophages.
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