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The activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), glutathione S-transferase (GST) as well as proline content were studi ed in leaves and roots of14 day-old pea plants treated with NiSO4 (10, 100, 200 pM) for 1,3, 6 and 9 day s. Exposure of pea plants to nickel (Ni) resulted in the decrease in CuZnSOD as well as total SOD activities in both leaves and roots. The activity of APX in leaves of plants treated with 100 and 200 pM Ni increased following the 3rd day after metal application, while in roots at the end of the experiment the activity of this enzyme was significantly reduced. In both organs CAT activity generally did not change in response to Ni treatment. The activity of GST in plants exposed to high concentrations of Ni increased, more markedly in roots. In both leaves and roots after Ni application accumulation of free proline was observed, but in the case of leaves conceniraiion of this amino acid increased eariier and to a greater extent than in roots. The results indicate that stimulation of GST activity and accumulation of proline in the tissues rather than antioxidative enzymes are involved in response of pea plants to Ni stress.
The influence of 50 and 100 µM Ni on the activities of nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), glutamate synthase (GOGAT), glutamate dehydrogenase (GDH), alanine aminotransferase (AlaAT) and aspartate aminotransferase (AspAT) was studied in the wheat roots. Root fresh weight, tissue Ni, nitrate, ammonium, glutamate and protein concentrations were also determined. Exposure to Ni resulted in a marked reduction in fresh weight of the roots accompanied by a rapid accumulation of Ni in these organs. Both nitrate and ammonium contents in the root tissue were considerably enhanced by Ni stress. While protein content was not significantly influenced by Ni application, glutamate concentration was slightly reduced on the first day after treatment with the higher Ni dose. Treatment of the wheat seedlings with 100 µM Ni led to a decrease in NR activity; however, it did not alter the activation state of this enzyme. Decline in NiR activity observed after application of 100 µM Ni was more pronounced than that in NR. The activities of GS and NADH-GOGAT also showed substantial decreases in response to Ni stress with the latter being more susceptible to this metal. Starting from the fourth day, both aminating and deaminating GDH activities in the roots of the seedlings supplemented with Ni were lower in comparison to the control. While the activity of AspAT remained unaltered after Ni application that of AlaAT showed a considerable enhancement. The results indicate that exposure of the wheat seedlings to Ni resulted in a general depression of nitrogen assimilation in the roots. Increase in the glutamate-producing activity of AlaAT may suggest its involvement in supplying the wheat roots with this amino acid under Ni stress.
It was found previously that methyl jasmonate (JA-Me) induced leaf abscission in Kalanchoe blossfeldiana. In present studies it was shown that JA-Me markedly increased the total activities of cellulase, polygalacturonase, pectinase and xylanase in petioles, but did not affect activities of these enzymes in the blades and apical part of shoots of K. blossfeldiana. These results suggest that methyl jasmonate promotes the degradation of cell wall polysaccharides in the abscission zone and in this way induces leaf abscission in Kalanchoe blossfeldiana. Aktywność glukanaz rozkładających ściany komórkowe w czasie indukowanego odpadania liści u Kalanchoe blossfeldiana przez ester metylowy kwasu jasmonowego.
Generation of superoxide anion (O2) and peroxidase activity were significantly increased in bean leaves infected with incompatible and compatible pathogens: Botrytis fabae and Botrytis cinerea, respectively, but the induction was greater on direct inoculation with B. fabae, than with B. cinerea. A slightly higher O2 level was also detected in the parts of leaves surrounding the inoculation side. Overproduction of Ó2 was observed earlier than the increase in peroxidase activity. Pretreatment of the leaves with methyl jasmonate enhanced both O2 production and peroxidase activity following inoculation with B. cinerea. Induction of superoxide dismutase activity after the infection was less pronounced than changes in Ó2 level. The differences in the rate of NADH oxidation in the extracts from control and inoculated leaves, correlated with the differences in the rate of O2 production. The results indicate thatCte level is one of the essential factors responsible for the difference in the interactions between bean plant and compatible and incompatible pathogens.
Effects of two Ni concentrations (50 and 100 µM) on growth, Ni accumulation as well as the activities of glutamine synthetase (GS) and glutamate dehydrogenase (GDH) were studied in shoots of wheat seedlings. Exposure to Ni caused rapid accumulation of this metal in the shoots accompanied by a substantial decrease in the length and fresh weight of these organs. Both aminating (NADH-GDH) and deaminating (NAD-GDH) glutamate dehydrogenase activities were significantly influenced by Ni stress, while GS activity did not change in response to Ni application. The activity of NADH-GDH showed an increase at the end of the experiment and 7 days after Ni treatment it was 68% and 76% higher than in the control, at 50 and 100 µM Ni, respectively. NAD-GDH activity after 1 and 4 days of exposure to a higher concentration of Ni was reduced by 24% and 37%, respectively. However, on the 7th day the activity of this enzyme was enhanced by 150% and 72% over the control level, at 50 and 100 µM Ni, respectively. The obtained results suggest that GDH can play an important role in response of wheat seedlings to Ni toxicity.
Behavioural problems and shyness are prevalent horse-related causes of accidents in equitation.The aim of the present study was to examine whether tests developed to measure the intensity of reaction to social separation and to novelty and suddenness can predict how riders perceive their safety when handling and riding a horse. Thirteen leisure horses were subjected to the battery of three fear tests, separation test and a test ride. The horses that were more fearful toward a novel surface were perceived to be less secure when handling. The horses showing fear in the startling object test were also perceived as less secure in both handling and riding. Unexpectedly, in the separation test, the more time the horse had remained a standing position, the more insecure when saddling, mounting, walking and trotting was scored. Similarly, a relaxed body posture with the head in a low position was predictive to a low security score by riders. I t may be supposed that the horses that are comfortable when isolated are self-reliant and independent in comparison to other horses, but probably also in other situations,which might make them difficult to be managed by humans.Behavioural tests could be predictive not only for equine personality traits, but also may be applied in the assessment of rider’s perceived safety. Thus, they may serve as tools to differentiate horses In terms of their suitability for leisure riders.
We have studied the influence of 50 sulfate/selenate ratios in the range of 2; 1.5; 1; 0.5; 0 mM/0.125; 0.25; 0.375; 0.5; 0.75; 1; 1.5; 2; 2.5; 3 mM on biomass growth, glucosinolate biosynthesis and selenium accumulation in T. majus and A. caucasica transformed roots producing Phe-derived glucotropaeolin and Met-derived glucoiberverin, respectively. Gradual decrease in glucosinolate production, more significant in glucoiberverin, was correlated both with the decrease in sulfate and increase in selenate concentrations and with increasing Se accumulation in the biomass of the roots. Both T. majus and A. caucasica transformed roots accumulated high amounts of Se in biomass, up to 2177 ± 81.7 µg·g⁻¹ DM and 3110 ± 97.4 µg·g⁻¹ DM, respectively, in the media with sulfate 0.5; 0 mM/selenate 2.5 mM ratios. In transformed roots cultured at sulfate 1.5; 1; 0.5; 0 mM/selenate 0.75; 1; 1.5; 2; 2.5; 3 mM ratios accumulation of Se into glucosinolate fractions occurred, it reached the maxima at sulfate 0.5 mM/selenate 2.5 mM ratio. In T. majus roots Se accumulation in glucosinolate fraction was by about 50% lower than in A. caucasica ones. These results indicated that selenium enriched hairy roots of T. majus and A. caucasica could be fully controlled sources of both glucosinolates and selenium as supplements for cancer chemoprevention.
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