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Studying protein-DNA binding by dynamic light scattering

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Zhelev N. Z., Buckle R. S., Snyder D., Marsh P. J.

Cellular and Molecular Biology Letters

1996

tom 01

nr 2

Light scattering experiments were undertaken to study binding of the Max transcription factor to its E-box DNA recognition sequence. Translational diffusion coefficients were measured and the average hydrodynamic radii (Rh) of complexes calculated using the Stokes-Einstein equation. We detected both dimerization of Max and the formation of a stable complex with its E-box DNA target. These results demonstrate the applicability of Dynamic Light Scattering for measuring protein-DNA interactions.

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Buckle R. S.

1 Marsh P. J.

1 Snyder D.

1 Zhelev N. Z.

1 1996

Wyniki wyszukiwania

Studying protein-DNA binding by dynamic light scattering