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Extreme weather conditions with prolonged dry periods and high temperature can severely influence grapevine physiology and morphology. Understanding the physiological and morphological responses of grapevines to water deficit is thus of utmost importance to modu-late the appropriate plant development. The present study evaluates the effects of deficit irriga-tion (DI) on certain leaf characteristics of grapevine cv. ‘Italia’ cultivated on different root-stocks. DI had remarkable effects on the growth, morphology, tissue structure, water status and physiology of grapevine leaf. Response of the ‘Italiaʼ cultivar to DI depended on rootstock used. For example, leaf fresh weight of ‘Italiaʼ/5 BB under DI decreased by 15.2% in compari-son to full irrigation (FI), whereas fresh weight values for ‘Italiaʼ/99 R and own-rooted vines under DI decreased 6.2 and 10.5%, respectively. Under FI treatment, stomatal conductance (gs) reached values of 189.0 mmol m-2 s-1 in ‘Italiaʼ/5 BB, and 178.8 mmol m-2 s-1 in ‘Italiaʼ/99 R. The gs values under DI condition were 178.1 and 164.0 mmol m-2 s-1 for the vines on 5 BB and 99 R respectively. Stomatal conductance decreased about 21.1, 13.8 and 10.2% in vines culti-vated on 5 BB, 99 R and own root, respectively. In response to DI, leaf relative water content decreased about 9.4, 4.1 and 3.9% for ‘Italiaʼ/5 BB vines, own roots, and ‘Italiaʼ/99 R, respec-tively. Combined data over years revealed that the vines cultivated on 99 R displayed more tol-erant leaf growth and physiology to drought in comparison to vines on 5 BB.
Few studies examined the stomatal conductance (gs) characteristics of grapevines with an emphasis on daily climatic responses. In the present study, diurnal measurements on leaf temperature and gs of three grapevine cultivars (Alphonse Lavallée, Crimson Seedless and Italia) were carried out. The leaf temperature values for the cultivars at 08:30 were 25.0 ±1°C and it increased to a maximum value between 12:00 and 14:50 p.m. After an almost steady course, it decreased along with the decrease in ambient temperature. The gs values increased from morning (08:30 a.m.) to mid-morning (10:30 a.m.) for all the cultivars. After reaching a peak level at mid-morning, the gs decreased gradually from the mid-morning throughout the afternoon. In the morning, the highest and the least gs values were obtained from Italia (232 mmol H2O m-2 s-1) and Crimson Seedless (149.6 mmol H2O m-2 s-1) cultivars. At around 10:30, the gs for Italia, Crimson Seedless and Alphonse Lavallée were at the highest levels with their valus 287.7, 262.1 and 242.0 mmol H2O m-2 s-1, respectively. The last measurements on gs at around 16:10 varied from to 96.7 (Italia) to 112.0 mmol H2O m-2 s-1 (Alphonse Lavallée). During the daily time course, the gs depended mainly on irradiance. Tleaf showed a strong relationship with Tair for all the cultivars. There was a strong, but negative correlation between leaf temperature and air humidity for all the cultivars.
Demand for fresh grape is increasing globally in accordance with the improvement in living standard since the grape berry contains large amounts of phytochemicals including anthocyanins, phenolics, flavonoids and resveratrol, which have been suggested to be responsible for human health benefits. However, table grapes easily undergo deterioration due to their soft texture and the high water content, which make it difficult to preserve without treatment. This study was thus conducted to evaluate the effect of preharvest calcium sprays on maintenance of postharvest quality of grapes (V. vinifera L. cv. ‘Thompson Seedless’). Three preharvest calcium sprays were applied to leaves and developing green berries with or without leaf removal pruning (a traditional practice performed in commercial vineyards worldwide) during berry development stages. After harvest, grapes were cold stored (1°C, 90% R.H.) up to 3 months. Preharvest micronized calcium sprays, with or without leaf removal pruning, markedly extended the postharvest quality of grapes by delaying weight loss, reducing decay, maintaining rachis chlorophyll concentrations and preserving visual quality during the prolonged cold storage. Besides, in calcite-treated grapes, lower titratable acidity decrease courses with a subsequent lower maturity index during prolonged storage indicate that calcite sprays restricted postharvest physiological senescence of grapes. Overall findings indicated that preharvest calcite sprays may be an environmental-friendly, healthy and sustainable viticulture practice for extending postharvest quality of grapes.
Testing the globally popular grapevine rootstocks for affinity with new grape cultivars or tolerance potential to stress factors like drought is essential for districts where supplemental irrigation is more frequently needed due to the pressures of a global climate change. In this study, a seasonal evaluation of leaf gas exchange, leaf temperature, leaf greenness (chlorophyll content prediction) and vegetative development of Vitis vinifera L. cv. ‘Prima’ grafted to different rootstock genotypes having great variability in drought tolerance, were investigated. The experiment was conducted in a controlled experimental glasshouse on two-year-old soilless grown ‘Prima’ vines grafted on nine rootstocks [44-53 M, 5 BB, 140 Ru, Ramsey (Syn. Salt Creek), 99 R, Saint George (Syn. Rupestris du Lot), 41 B, 1613 C and 420 A]. A long-term deficit irrigation (DI) was imposed before bud break by reducing water supply to 40% of field capacity derived from concurrent measurements of water content of growth medium and maintained until the end of vegetation period. The rootstocks significantly modulated the physiology and vegetative growth of the scion cultivar in varying degrees according to their genetic features. Among them, 140 Ru rootstock found to be more prominent in terms of mitigating the adverse effect of water deficit on physiology and growth of the scion genotype ‘Prima’ as there were no significant difference between deficit and full irrigation treatments for most of parameters investigated. In general, the ‘Prima’ scion performed better when the rootstocks coming from V. berlandieri × V. rupestris pedigree rather than the others including V. berlendieri × V. riparia.
Ever-increasing shortage in global agricultural water sources urged the researchers to investigate sustainable strategies for alleviating the negative effects of drought on plants in semi arid or arid regions. In this sense, foliar fertilization gained particular significance as it supports the plant to cope with water shortage. This study was conducted to investigate the leaf physiological response of the grapevine ‘Narince’ to various organic leaf fertilizers. Leaf protein content was also determined to compare treatment effects on nutritional value of grape leaves since the leaves of this cultivar are consumed in various ways. Both stomatal conductance, leaf chlorophyll and protein content increased in response to leaf fertilizers in varying degrees according to the products used. To illustrate, Herbagreen pulverization resulted in the highest stomatal conductance (gs) (324.7 mmol H₂O m- ² s -¹), and was followed by Cropset (323.5 mmol H₂O m- ² s - ¹) while the lowest gs value was obtained from control vines (295.4 mmol H₂O m- ² s - ¹). The highest chlorophyll and protein contents were obtained from ISR 2000 (32.9 mg kg- ¹) and Maxicrop (21.5%) treatments, respectively. Leaf chlorophyll content had a significant positive correlation with leaf protein content when all the observations were pooled. Therefore, the organic leaf fertilizers can be considered as a safe, sustainable and innovative strategies to support plants to cope with drought.
Blackberries (Rubus spp.) have significant nutraceutical properties, providing phenolic compounds for the consumers. These compounds are useful to human health due to their high antioxidant activity. However, most blackberries destined for fresh markets become unmarketable in 2 to 3 days due to decay and leakage, because they have thin and fragile skin. The present study was aimed to investigate the effect of salicylic acid (SA) and calcium chloride (CaCl2) on extending the postharvest quality and bioactive compounds of blackberry during refrigerated storage. Blackberry fruits cv. ‘Jumbo’ were dipped in 2% CaCl2, or in 2.0 mM salicylic acid (SA) for 10 min. Total soluble solids contents, titratable acidity, pH, ascorbic acid contents, total phenolics contents, and total antioxidant activity were investigated initially on 4th, 7th, or 10th day (d) of storage. Changes in fresh weight, titratable acidity, ascorbic acid and total phenol contents were markedly delayed by both treatments. Berries treated with 2 mM SA or 2% CaCl2 exhibited markedly better visual quality during the storage period. Considering the overall findings, this study revealed that postharvest SA or CaCl2 applications maintained the storage-life and conserved the valuable marketing features of blackberries over 10 d in cold storage, presumably due to their inhibitory effects on fruit senescence.
High carbonate content in soil negatively affect plant growth, because the availability of nutrients is restricted due to high pH. The present investigations were carried out to reveal possible alleviating effects of the exogenous root inoculation PGPRs on development and physiology of soilless-grown grapevines cultivated under alkaline stress in controlled glass house. pH of growth medium was increased from 7.5 to the values ranging from 7.9 (control) to 8.1 (A18) according to the bacterial inoculations by NaHCO3 supplementations. Bacteria inoculations did not result in statistically significant differences in pH values of growth media. The bacterial population density found in the rhizosphere of grapevines ranged from 6 × 108 CFU mL−1 (M-3) to 9 × 108 CFU mL−1 (Ca-637). The highest value of shoot thickness was obtained from Ca-637 (5.3 mm), followed by A18 (5.2 mm), while M3 did not significantly affected the shoot thickness. The greatest pruning residue per vine was obtained from A18 treatment (81.5 g), followed by Ca-637 (80.8 g) while the lowest value was determined in control. Vine yield was the greatest with A18 (1128 g) treatment and was followed by Ca 637 (1059 g). Considering the general observations, root inoculation of PGPRs A18 and Ca-637 may be recommended in enhancing bioremediation of alkali growth media.
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