The studies on the occurrence and species composition of entomopathogenic nematodes (Steinernematidae, Heterorhabditidae) in chemically protected and unprotected were carried out in 2009–2011. Field studies conducted in 2009–2011, in orchards without protection and where chemical treatments were performed evenly, according to recommendations. The aim of this study was to compare the frequency and species composition of entomopathogenic nematodes (Steinernematidae, Heterorhabditidae) in orchards protected and unprotected chemically. Soil samples were collected from selected sites three times (spring, summer, autumn) in the season. Four species of entomopathogenic nematodes three of the families Steinernematidae and Heterorhabditidae were isolated from soil samples. The results of the studies revealed both a higher biodiversity of the nematode species composition as well as their incidence in the enprotected apple orchards.
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