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Transformation through agroinfection on decapitated shoot apex of field-growing Phyllanthus amarus

100%

Arya A. K., Martin K. P., Sabovljevic A., Madassery J.

Acta Physiologiae Plantarum

2011

tom 33

nr 5

A simple and easy transformation strategy was accomplished on field growing plants of Phyllanthus amarus, an anti-hepatitis B drug plant. Infection of Agrobacterium rhizogenes strains A₄M70GUS and ATCC 15834 on decapitated shoots of field growing P. amarus induced hairy roots and crown gall, respectively. Infection with A₄M70GUS yielded a mean of 23.2 roots from 40% plants in 40-day period. The crown gall induced on 30% plants after infection with ATCC 15834 grew to 5–10 mm in diameter. The roots and crown galls established in vitro on Murashige and Skoog (MS) basal medium grew well. The hairy roots yielded fivefold (6.91 g) biomass in halfstrength MS liquid medium to that of the adventitious roots derived from internode explants in MS medium with 8.0 µM α-naphthaleneacetic acid (1.39 g). Histochemical assay and PCR analysis using the primers of uidA coding region confirmed the hairy roots induced by A₄M70GUS. The crown galls induced by ATCC 15834 were confirmed by PCR analysis using rolB gene primers. The protocol enables an easy and early accomplishment of hairy roots.

Ograniczanie wyników

1 Acta Physiologiae Plantarum

1 Arya A. K.

1 Madassery J.

1 Martin K. P.

1 Sabovljevic A.

1 2011

Wyniki wyszukiwania

Transformation through agroinfection on decapitated shoot apex of field-growing Phyllanthus amarus