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The aim of this research was to investigate the addition of Calcium source (marble powder) of up to 1.2, 1.4, 1.6% levels on growth performance, feed intake, some organ weights and tibia ash parameters in broiler diets. This research was conducted according to Randomized Plots Design by using 3 groups with 3 replicates between 12 May - 23 June in Çarýklý region of Diyarbakýr. The experiment was conducted on 126 day-old equally sexed Ross 308 broiler chicks. Three diets used as 3 treatments were included (1.2, 1.4 and 1.6% Ca levels) in broiler the diets. Three replicates with 14 equally sexed birds were arranged in each plot. The experiment was started from 7-days-of-age to slaughter weight. The first group was a control and contained 1.2% Ca levels; marble flour was added to the control diet as a Ca source and the Ca levels 1.4 and 1.6% for the 2nd and 3rd groups, respectively. At the end of the trial, data were analysed by MSTAT C program, and means were examined by DUNCANs multiple test. There were no statistically significant differences between the groups for all investigated parameters (Live weight gain, feed intake, feed conversion ratio, carcass parameters and some organ weights) using Ca doses (P > 0.05). However, crude ash and calcium contents of diets were increased by additional Ca levels (P < 0.01), but phosphorus content of diets were decreased by the addition of a calcium source. The results of this study have shown that there is no adverse effect on broiler performance of an additional Ca source up to 1.6% levels.
Ghrelin is a peptide hormone which plays important role in maintaining growth hormone release and energy homeostasis in vertebrates. Spirulina platensis (SP) has antioxidant and hypolipidemic effects due to its ingredients. In this study we aimed to investigate the effects of SP on the testicular structure and relation between ghrelin and testosterone in the testis of rats fed a high fat diet (HFD). Sixty four young adult male rats were used and divided to 8 equal groups. Experimental groups received addition of 10% cholesterol (CHL), 43% hydrogenated vegetable oil (HVO) and 3% SP alone or in combination to basal diet while the control group received only basal diet. Serum ghrelin and testosterone levels were measured with ELISA. Receptors for ghrelin and androgen were detected with immunohistochemistry. For histomorphometric investigation, tubulus seminiferus, intertubular area, tubulus seminiferus lumen, Leydig cell nucleus, Sertoli cell nucleus, germ cell nucleus, spermatocyte nucleus and elongated spermatid volume densities were determined stereologically. Serum ghrelin level was increased especially in HVO and CHL combination group compared to the control while serum ghrelin levels were close to control levels in SP-received groups. Ghrelin receptor level was increased in tubulus seminiferus with HVO+CHL administration but this effect was, however, limited in HVO+CHL and SP challenged groups. HVO+CHL administration caused a significant decrease in Leydig cell nucleus volume density, as well as in all SP-received groups, compared to the control. Significantly increased spermatocyte nucleus volume density in cholesterol-receiving groups was decreased to control level with SP alone and its combinations.
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