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1

Transfer to wheat [Triticum aestivum] of small chromosome segments from rye [Secale cereale] carrying disease resistance genes

100%
Fu S., Tang Z., Ren Z., Zhang H.
Journal of Applied Genetics
|
2010
|
tom 51
|
nr 2
115-121
One hundred wheat lines, derived from monosomic additions of chromosome 1R of rye inbred line R12 (Chinese rye), were detected by PCR amplification using rye-specific primer pairs. Only 5 wheat lines, 1R296, 1R330, 1R314, 1R725,and 1R734, were determined to contain rye chromatin. While 1R296 and 1R330 were highly susceptible to stripe rust and powdery mildew, 1R314, 1R725 and IR734 were highly resistant to both diseases. Acid-polyacrylamide gel electrophoresis showed that the ω-secalin bands were absent in 1R314, but present in the other 4 wheat lines. Genomic in situ hybridization indicated that 1R296, 1R330, and 1R725 contained translocations involving the whole short arm of chromosome 1R. However, 1R314 and 1R734 contained a pair of wheat chromosomes with small, terminal, rye-derived chromosome segments. The results suggest that the translocation breakpoint of 1 RS in 1R314 was located between the Sec-1 locus and the disease-resistance loci, while in line 1R734, the breakpoint was located between the Sec-1 locus and the centromere. Taking account of the improved disease resistance of 1R725,1R314 and 1R734, the chromosome arm 1 RS of R12 may represent new and valuable disease resistance resources for wheat improvement.
2

Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis

100%
Tang Z., Yang Z., Fu S.
Journal of Applied Genetics
|
2014
|
tom 55
|
nr 3
3

Four new earthworm species belonging to Amynthas Kinberg and Metaphire Sims et Easton (Megascolecidae: Oligochaeta) from Guangdong, China

100%
Zhang W., Li J., Fu S., Qiu J.
Annales Zoologici
|
2006
|
tom 56
|
nr 2
In this study, we described two new species of the genus Amynthas with one pair of spermathecal pores in segment 8/9 from the riparian forest in Mt. Dinghu and two new Metaphire species with two pairs of spermathecal pores in segment 6/7-7/8 from a coniferous forest and an evergreen broadleaf forest in the Dadingshan station of the Nanling National Natural Reserve, Guangdong, China. The former two Amynthas species appear to be closely related to A. antefixus (Gates, 1935), Sichuan, China. However, A. dirighumontis is much smaller, and its slightly convex ellipse-shaped male porophores are in XVIII or XVII about 0.17-0.25 body circumferences ventrally apart. In addition, there is always a slight twist at about midway of the spermathecal duct from where the slender, twisted diverticulum passed into. Finally, there are no genital markings or papillae in male pore and spermathecal pore regions, instead of the presence of a median, presetal genital marking at the midventral line on segments III, IV and V as in A. antefixus. As to Amynthas liaoi sp. nov., it is characterized by the constant presence of two horizontal rows of three to eight presetal and postsetal papillae in segment XVIII, and one horizontal row of about five postsetal papillae in segment IX. The ampulla shape and size are also different between this new species and A. antefixus. The two Metaphire species are M. nanlingmontis sp. nov. and M. dadingmontis sp. nov. M. nanlingmontis sp. nov. appears to be related to M. jianfengensis (Quan, 1985). They have the same spermathecal pore number and position, and similar male pore cop- ulatory chambers. However, M. nanlingmontis is much smaller in body size than M. jianfengensis. In addition, there is a special ring-like swelling at the border between the duct and the chamber in the spermathecal diverticulum, and a mushroom-like accessory gland present beside the duct. M. dadingmontis sp. nov. appears to be closely related to M. nanlingmontis sp. nov. However, it is also easy to distinguish them from their different diverticulum shape and accessory genital glands in prostatic region. A comparison among the 10 similar Metaphire species with two pairs of spermathecae in VII and VIII has been made.
4

Temporal and spatial patterns of aboveground biomass and its driving forces in a subtropical forest : a case study

76%
Ma L., Li W., Shi N., Fu S., Lian J., Ye W.
Polish Journal of Ecology
|
2019
|
tom 67
|
nr 2
5

The novel Na+/H+ antiporter gene SpNHX1 from Sesuvium portulacastrum confers enhanced salt tolerance to transgenic yeast

64%
Zhou Y., Yang C., Hu Y., Yin X., Li R., Fu S., Zhu B., Guo J., Jiang X.
Acta Physiologiae Plantarum
|
2018
|
tom 40
|
nr 03
6

Isolation and characterization of a C-repeat binding factor (CBF)-like gene in cassava (Manihot esculenta Crantz)

52%
Li R., Ji Y., Fan J., Yang C., Yao Y., Zhou Y., Duan R., Liu J., Fu S., Hu X., Guo J.
Acta Physiologiae Plantarum
|
2014
|
tom 36
|
nr 11
Cassava (Manihot esculenta Crantz) is a tropical and subtropical plant and susceptible to chilling injury. In this research, a C-repeat binding factor (CBF)-like gene (GenBank accession number JQ339740) has been isolated from cassava, and named as MeCBF1. The full-length DNA of MeCBF1 is 1,037 base pair (bp), without intron. The 5' untranslated region is 102 bp, the 3' untranslated region is 239 bp, and the open reading frame is 696 bp encoding 231 amino acids. The deduced amino acid sequence of MeCBF1 contains two CBF conserved motifs of PKK(P/R)AGRxKFxETRHP and DSxWR. The MeCBF1 shows 83 % homology to the CRT/DRE binding factor 1 from Hevea brasiliensis (Accession no. AAY43213.1). However, in cassava, the MeCBF1 target genes showed low similarity to the CBF/DREB regulated genes in Arabidopsis thaliana. Quantitative real-time PCR showed that the MeCBF1 was highly expressed in stems and leaves, and lowly expressed in roots. In addition, the expression of the MeCBF1 quickly responded to low temperature stress (4°C). These results suggest that, the MeCBF1 is functional in cassava. Further studies on the MeCBF1 might be helpful to reveal molecular mechanism of cassava’s high sensitivity to low temperature.
7

Frequency of double minute chromosomes and combined cytogenetic abnormalities and their characteristics

52%
Fan Y., Mao R., Lv H., Xu J., Yan L., Liu Y., Shi M., Ji G., Yu Y., Bai J., Jin Y., Fu S.
Journal of Applied Genetics
|
2011
|
tom 52
|
nr 1
Double minute chromosomes (DMs) are the cytogenetic hallmark of extra-chromosomal genomic amplification. The frequency of DMs in primary cancer and the cytogenetic features of DMs-positive primary cancer cases are largely unknown. To unravel these issues, we retrieved the Mitelman database and analyzed all DMs-positive primary cancerous karyotypes (787 karyotypes). The overall frequency of DMs is 1.4% (787 DMs-positive cases; total 54,398 cases). We found that DMs have the highest frequency in adrenal carcinoma (28.6%, topography) and neuroblastoma (31.7%, morphology). The frequencies of DMs in each tumor were much lower than in previous reports. The frequency ofDMs in malignant cancers is significantly higher than in benign cancers, which confirms that DMs are malignant cytogenetic markers. DMs combined cytogenetic abnormalities are identified and sorted into two groups by principal component analysis (PCA), with one group containing −4, −5, −8, −9, −10, −13, −14, −15, −16, −17, −18, −20, −21, and −22, and the other containing −1p, −5q, +7, and +20. The prominent imbalance in DMs-positive cancer cases is chromosome loss. However, DMs-positive cancer cases, deriving from different morphologic cancers, cannot be clearly divided into subgroups. Our large database analysis provides novel knowledge of DMs and their combined cytogenetic abnormalities in primary cancer.
8

A Survey on How Ocular Surface Demodex Infestation Interactively Associates with Diabetes Mellitus and Dry Eye Disease

52%
Huang C., Chen S., Fu S., Li Y., Li Z., Li S., Liang X., Wang Z., Wang Z., Chen Y., Deng Q., Yi G., Fu M.
Acta Parasitologica
|
2021
|
tom 66
|
nr 3
9

Effect of male sterile and fertile cytoplasm on nuclear DNA methylation in hybrid rice

52%
Ali A., Li Y., Chen H., Xu P., Zhang H., Chen X., Yongxiang L., Fu S., Wu T., Iqbal M. Z., Farooq M. U., Wu X.
Acta Physiologiae Plantarum
|
2019
|
tom 41
|
nr 06
Nucleus-controlled fertility restoration and cytoplasmic male sterility are important mechanisms to exploit heterosis. However, the effect of DNA methylation on cytoplasmic-nuclear interaction is not well understood yet. The current study used a methylation-sensitive amplified polymorphism to characterize polymorphism in nuclear DNA methylation among cytoplasmic male sterile line (D62A), corresponding maintainer line (D62B), and two F1 hybrids (D62A × R527 and D62B × R527). In results, 495 fragments were amplified between the parental D62A and D62B lines. The total methylation (double + single-stranded) and full methylation (double-stranded) rates of D62A (33.13%, 24.24%) both were found to be lower than that of corresponding maintainer D62B (33.94%, 24.85%). Analysis of methylation revealed that male sterile line D62A was less methylated than that of corresponding maintainer line D62B in all methylation types I, II and III. A total of 516 fragments were amplified between two F1 hybrids (D62A × R527 and D62B × R527). The total methylation in both hybrids (D62A × R527 and D62B × R527) was identical (34.69%). While full methylation rates for D62A × R527 and D62B × R527 were 25.78% and 25.58%, respectively, that is non-significant. Moreover, polymorphism in DNA methylation was found higher in F1 hybrids (5.43%) than parents (4.24%). These results implied that different cytoplasm leads to changes in nuclear DNA methylation and sterile cytoplasm has reduced the effect on nuclear methylation than non-sterile cytoplasm. Current study explains the interaction between cytoplasmic male sterility and DNA methylation which may contribute to further research.
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