Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Polymorphisms of the interleukin-15 gene and their associations with fatness and muscle fiber traits in chickens

100%

Lv S.-J., Su L., Li H., Han R.-L., Sun G.-R., Kang X. T.

Journal of Applied Genetics

2012

tom 53

nr 4

Interleukin-15 (IL-15) is a cytokine that has been proposed to modulate skeletal muscle and adipose tissue mass. In the present study, an F2 resource population of Gushi chickens crossed with Anka broilers was used to investigate the genetic effects of the chicken IL-15 gene. Two single nucleotide polymorphisms (SNPs) (g.31224G>A and g.31266T>G) were identified in exon 5 of the IL-15 gene by means of polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing. Associations between the two SNPs and chicken fatness and muscle fiber traits were determined using linkage disequilibrium, haplotype construction, and association analysis. Both of the SNPs were associated with abdominal fat weight, leg muscle fiber diameter, and leg muscle fiber density (p < 0.05). Haplotypes of the two linked SNPs were associated with abdominal fat weight, fat thickness under the skin, and leg muscle fiber diameter (p < 0.05). The results suggested that the IL-15 gene might be associated with the causative mutation or the quantitative trait locus (QTL) controlling the fatness traits and muscle fiber traits in chickens.

MicroRNAs-1614-3p gene seed region polymorphisms and association analysis with chicken production traits

100%

Li H., Sun G.-R., Tian Y.-D., Han R.-L., Li G.-X., Kang X.-T.

Journal of Applied Genetics

2013

tom 54

nr 2

In the present study, a total of 860 chickens from a Gushi–Anka F2 resource population were used to evaluate the genetic effect of the gga-miR-1614-3p gene. A novel, silent, single nucleotide polymorphism (SNP, +5 C>T) was detected in the gga-miR-1614-3p gene seed region through AvaII polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) and PCR products sequencing methods. Associations between the SNP and chicken growth, meat quality and carcass traits were performed by association analysis. The results showed that the SNP was significantly associated with breast muscle shear force and legmuscle water loss rate, wing weight, liver weight and heart weight (p<0.05), and highly significantly associated with the weight of the abdominal fat (p<0.01). The secondary structure of gga-miR-1614 and the free energy were altered due to the variation predicted by the M-fold program.

Ograniczanie wyników

2 Journal of Applied Genetics

2 Han R.-L.

2 Li H.

2 Sun G.-R.

1 Kang X. T.

1 Kang X.-T.

1 Li G.-X.

1 Lv S.-J.

1 Su L.

1 Tian Y.-D.

1 2013

1 2012

Wyniki wyszukiwania

Polymorphisms of the interleukin-15 gene and their associations with fatness and muscle fiber traits in chickens

MicroRNAs-1614-3p gene seed region polymorphisms and association analysis with chicken production traits