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Presentation of beta-galactosidase activity secreted by plant cells

100%

Stano J., Micieta K., Grancai D., Blanarikova V.

Herba Polonica

2007

tom 53

nr 1

Extracellular plant β-galactosidase from opium poppy and celandine was detected on agar plates by the presence of dyed zones from 1-naphthyl-β-D-galactopyranoside used as a synthetic substrate. Evaluating the azo-dye zones assessed the degree β-galactosidase activity. No coloration of the agar medium was observed on non-inoculated parts, in medium inoculated with heat – inactivated cells (10 min. at 100oC) or in medium without substrate with all calli tested. On the agar plates with substrate and sterile opium poppy, celandine or gherkin seedlings (2–6 days old) changes in coloration were observed showing the release of β-galactosidase from the roots during germination.

Immunomodulating activity of leaf decoctions from four Staphylea L. species

88%

Lacikova L., Urbancikova B., Bukovsky M., Masterova I., Grancai D.

Herba Polonica

2007

tom 53

nr 4

Immunomodulating activity of leaf decoctions from four Staphylea species (Staphylea colchica Stev., S. elegans Zab., S. holocarpa Hemsl. and S. pinnata L.) was studied on human mononuclear cells. Phagocytic activity of mononuclear cells was highest after incubation with the extract of S. holocarpa Hemsl. S. colchica Stev. possesses the highest lysozyme activity, a little lower values were determined by S. holocarpa Hemsl. and S. pinnata L. The inhibition of the peroxidase activity of mononuclear cells was most significant in S. holocarpa Hemsl., in opposition on S. pinnata L., the only species that showed the stimulating effect on the immune cells again. All the studied species showed the immunomodulating activity, S. pinnata L. seems to be immunostimulating only. S. colchica Stev. and S. holocarpa Hemsl. are the most prospective species from the view of immunomodulating activity.

Ograniczanie wyników

2 Herba Polonica

2 Grancai D.

1 Blanarikova V.

1 Bukovsky M.

1 Lacikova L.

1 Masterova I.

1 Micieta K.

1 Stano J.

1 Urbancikova B.

2 2007

Wyniki wyszukiwania

Presentation of beta-galactosidase activity secreted by plant cells

Immunomodulating activity of leaf decoctions from four Staphylea L. species