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1

Rola wirusa BPIV-3 w chorobach układu oddechowego u bydła

100%
Larska M.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2020
|
tom 15
|
nr 2
2

Problematyka Międzynarodowego Sympozjum na temat EVA. Czy w Polsce potrzebny jest program zwalczania wirusowego zapalenia tętnic koni?

100%
Larska M.
Życie Weterynaryjne
|
2009
|
tom 84
|
nr 01
3

Sytuacja epizootyczna zakażeń wirusem Schmallenberg w Polsce

100%
Larska M.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2015
|
tom 10
|
nr 2 Monografia
4

Wirus Schmallenberg - sytuacja w Polsce

75%
Larska M.
Top Agrar Polska
|
2014
|
nr 03 Top Bydło
5

Zastosowanie cytometrii przeplywowej do oceny wrazliwosci wybranych linii komorkowych na zakazenie wirusem zapalenia tetnic koni

63%
Larska M., Rola J.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 01
105-109
The purpose of the study was to attempt the application of flow cytometry to evaluate the sensitivity of various cell lines to EAV infections, according to their type and passage number. Monolayers of RK13, Vero, BHK-21 and MDBK cells were infected with reference EAV strain Bucyrus. First of all the susceptibility of each cell line to different titers of the virus was tested. The second step was to establish a time course kinetic of viral infection. Sequentially, starting from 2 to 72 hours post infection the cells were fixed, permeabilized and stained with FITC monoclonal EAV-specific antibodies. The analysis carried out in Coulter Epics XL (Beckman Coulter) flow cytometer considered the percentage of EAV infected cells determined in a time range by gating FITC/Count histograms. Significant differences in the sensitivity to EAV infection in particular cell lines were found. After 24 h. p.i. most RK13 cells infected with Bucyrus strain showed signs of infection in the titer of 10 TCID₅₀, whilst in Vero and BHK-21 a similar histogram was not obtained until 100 and 1000 TCID₅₀ respectively. As early as 10 and 12 h post inoculation significant level of infected cells (37.9 and 18.6%) were detected in line RK13 passage 36 and 11 respectively. Cell line Vero passage 115 indicated a higher sensitivity to EAV infection comparing to Vero passage 153. More than 70% of cells from that line were EAV infected after 24 hours post inoculation. In Vero passage 153 the infected cells (15.4%) were not detected until 36 h.p.i. The presence of infected cells was found also after 36 h post inoculation in BHK-21 cell line passage 10. The obtained results indicate that differences in cell line susceptibility to EAV infection depend on their type and decrease with their passage.
6

Zastosowanie real time RT-PCR do wykrywania wirusa biegunki bydła i choroby błon śluzowych (BVD-MD) typu 1 i typu 3 w materiale biologicznym

63%
Larska M., Polak M. P.
Medycyna Weterynaryjna
|
2011
|
tom 67
|
nr 07
The aim of the study was to use real time RT-PCR for the detection of genetic material of bovine viral diarrhea virus (BVDV) type 1 and type 3 in serum and milk samples. Material tested included the fetal calf serum used for cell culture, serum samples from healthy calves and from calves experimentally inoculated with BVDV type 1 and type 3, and milk samples (pasteurized and treated with ultra high temperature). Sensitivity of the test was 200 copies of RNA per reaction (10⁵ viral RNA copies per ml) for both types of BVDV, using dedicated primers and standards. Detection limit was 10² tissue culture infectious dose 50 (TCID₅₀) and 1 TCID₅₀ for type 1 and type 3, respectively. Diagnostic specificity of the method was 100%. Out of 10 samples of milk, 3 were positive for BVDV type 1, while none was positive for type 3. On the other hand, BVDV type 3 was found in 6 out of 10 samples of fetal calf serum. Real Time RT-PCR for BVDV type 1 and type 3 proved to be a highly sensitive and highly specific technique, enabling the detection of viral genetic material in various samples, even when its detection by virus isolation or antigen ELISA tests is impossible because of virus inactivation by such processes as high temperature, gamma irradiation, or the presence of virus neutralizing antibodies.
7

Odkrycie homologu wirusa zapalenia wątroby typu C u psów

63%
Larska M., Larski W.
Magazyn Weterynaryjny
|
2012
|
tom 21
|
nr 04
8

Zmiennosc genetyczna wirusa a diagnostyka zakazen EAV

63%
Larska M., Rola J.
Medycyna Weterynaryjna
|
2006
|
tom 62
|
nr 12
1348-1351
Equine arteritis virus (EAV) belongs to the family Arteriviridae of the order Nidovirales. The viral genome composed by single-stranded positive sense RNA is enclosed in a icosahedral nucleocapsid and surrounded by a proteolipid envelope. The genome consists of nine open reading frames (ORFs) coding both structural and non-structural proteins. Although only one serotype of EAV is distinguished, field isolates differ in virulence and pathogenicity. The EAV infection is usually subclinical. 30-60% of stallions after infection become persistent carriers of the virus and can shed EAV with their semen during next several weeks, months or even years. Mares covered by EAV shedding stallions can result in abortions, fetus resorptions, infertility and even death of the newborn foals that lead to large losses in horse breeding. Seropositive stallions sheding the virus in their semen are the main reservoir of the virus; the control of the disease, therefore, should be based on their identification and elimination from breeding. Genetic variability of EAV can lead to the increase of virulence of the isolates and to changes in viral properties having an impact on the results of laboratory testing. Researching genetic modification of the viral genome provides important information about the changes in the nucleotide sequence of currently circulating strains and about the direction of EAV evolution. The purpose of the review is to present current data concerning molecular biology and diagnostics of equine arteritis virus infections.
9
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Zwierzęta wolno żyjące jako rezerwuar wirusa zapalenia wątroby typu E w Polsce

63%
Larska M., Larski W.
Życie Weterynaryjne
|
2016
|
tom 91
|
nr 05
10
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Detection of Schmallenberg virus RNA in bull semen in Poland

63%
Kesik-Maliszewska J., Larska M.
Polish Journal of Veterinary Sciences
|
2016
|
tom 19
|
nr 3
The detection of Schmallenberg virus (SBV) in the breeding bull semen raised the question of the possibility of venereal transmission of SBV which could result in cost-intensive restrictions in the trade of bovine semen. In order to evaluate the presence of SBV RNA in bovine semen, 131 bull semen samples from four locations in Poland collected between 2013 and 2015 were analysed by RT-PCR for viral RNA. SBV RNA was detected in 5.3% of the samples. The study has revealed that application of an appropriate RNA extraction method is crucial to detect virus excretion via semen.
11

Immobilizacja farmakologiczna żubrów

63%
Krzysiak M. K., Larska M.
Medycyna Weterynaryjna
|
2014
|
tom 70
|
nr 03
Białowieża National Park (BNP) takes care of free-ranging bison herds, as well as captive bison reserves. Bison, as a non-domestic animal, requires general immobilization for the purposes of diagnostic sample collection, thorough clinical examination, treatment, and preventive interventions. The paper presents the results of 110 successful immobilizations of European bison, performed between 2009 and 2013 in the Bison Breeding Centre of the BNP. Depending on the use of specific drugs and additional substances for premedication, such as xylazine, immobilized animals were divided into three groups. The preparations were administered with specialized pneumatic Dan-Inject applicators. Experience shows that the use of preparations containing etorphine is safe for both the patient and the personnel after the immobilization of the animal. Following a successful shot, the animal is immobilized within 15 minutes. General pharmacological immobilization is necessary for the efficient and safe performance of veterinary and animal husbandry manipulations, such as blood, swab, and tissue biopsy sampling.
12

Zastosowanie testu immunoperoksydazowego posredniego do potwierdzania tozsamosci izolatow EAV

63%
Larska M., Rola J.
Medycyna Weterynaryjna
|
2007
|
tom 63
|
nr 06
712-716
The purpose of the study was the elaboration and introduction of an effective method to confirm the identity of EAV isolates obtained in cell culture from the serum of persistently infected stallions. In view of the low cost, simplicity of preparation, and its high specificity, an indirect immunoperoxidase test based on rabbit polyclonal sera was introduced. After immunization of the rabbits with the EAV reference strain Bucyrus, purified by ultracentrifugation, a specific polyclonal serum in the titer of SN antibodies from 1:32 to 1:128 was obtained. Peroxidase-conjugated goat IgG antibodies against horse immunoglobulins were used as a secondary antibody and DAB as a substrate. After the optimalization of the test conditions, its sensitivity and specificity was defined. The sensitivity estimated in reference to the Bucyrus strain was equal to 1 TCID₅₀. The specificity was defined at 100%. After the preliminary validation, the indirect immunoperoxidase test based on the obtained polyclonal sera was affirmed as a method to identify EAV isolates. All 68 EAV field isolates reacted positively in the immunoperoxidase test.
13

BVDV-2 - nowe czy stare zagrożenie?

63%
Larska M., Antos
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2020
|
tom 15
|
nr 1
14

Ryzyko wystąpienia chorób przenoszonych przez wektory owadzie u zwierząt w Europie

63%
Krason K., Larska M.
Postępy Mikrobiologii
|
2018
|
tom 57
|
nr 4
15

COVID-19 w aspekcie weterynaryjnym

63%
Larska M., Krzysiak M. K.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2020
|
tom 15
|
nr 2
16

Zroznicowanie genetyczne szczepow EAV wsrod trwale zakazonych ogierow w stadach ogierow w Polsce

63%
Larska M., Rola J.
Medycyna Weterynaryjna
|
2007
|
tom 63
|
nr 11
1381-1386
The purpose of the study was to analyse the genetic diversity of Polish EAV isolates. Genetic variability can lead to increased virulence of isolates and to significant changes in EAV antigen properties influencing the results of laboratory testing. Studies on genetic modifications of viral genomes as well as on the phylogenetic affinity of strains have facilitated the investigation of viral evolution. Phylogenetic analysis was performed on 32 isolates that were isolated from the semen of asymptomatic virus-shedding stallions originating from 8 national studs. These isolates were compared with 15 reference EAV strains commonly used in phylogenesis. On the basis of the nucleotide sequence analysis of ORF5 gene encoding GP5 glycoprotein it was shown that Polish EAV isolates belonged to two subgroups and demonstrated the closest relationship to the European strains. None of these strains had any relationship to the first Polish strain Wroclaw-2 isolated in 1976. The homology of ORF5 nucleotide and predicted GP5 amino-acid sequences of Polish isolates attained a level of 81.2-99.0% and 90.1-99.4% respectively. Analyzing the genetic diversity of ORF5 facilitated the conducting of retrospective epizootic investigations.
17

Rola krwi w transmisji chorob prionowych

63%
Larska M., Polak M. P.
Medycyna Weterynaryjna
|
2003
|
tom 59
|
nr 08
670-672
18

RT-PCR as effective detection method of equine arteritis virus in the semen of naturally infected stallions

63%
Larska M., Rola J.
Bulletin of the Veterinary Institute in Puławy
|
2003
|
tom 47
|
nr 2
19
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Co dalej z wirusem Schmallenberg?

51%
Kesik-Maliszewska J., Larska M., Rola J.
Życie Weterynaryjne
|
2023
|
tom 98
|
nr 02
20

Atypowe pestiwirusy - ryzyko dla hodowli bydła i znaczenie dla kontroli wirusowej biegunki bydła i znaczenie dla kontroli wirusowej biegunki bydła i choroby błon śluzowych (BVD-MD)

51%
Kuta A., Larska M., Polak M. P., Zmudzinski J. F.
Medycyna Weterynaryjna
|
2012
|
tom 68
|
nr 02
The paper presents the current state of knowledge on the prevalence and risk of a new type of bovine viral diarrhea virus known as type 3 (BVDV-3). The first discovered atypical pestivirus was the isolate D32/00_’HOBI’, detected in fetal calf serum (FCS) originating from Brazil. The isolates CH-KaHO/cont, SVA/cont-08 and IZSPLV_To are further examples confirming the presence of BVDV type 3 in the FCS. This new species of pestiviruses (BVDV-3) is a problem not only for research laboratories using bovine serum, but also for cattle breeders. Natural infections with this virus have been reported in Brazil, Thailand and Italy, which may suggest that the new species of pestivirus is also present in European cattle. The methods used for routine diagnosis of BVDV infection are ineffective in detecting atypical pestiviruses, which may pose a risk of false negative results. It may also influence the safety of vaccines and biological products produced on the basis of contaminated batches of fetal bovine serum.
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