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Within the Ocimum genus there occur about 200 species in different varieties and forms. They differ in the essential oil content and quality, as well as in many morphological features. The studies conducted in the years 2008–2009 were aimed at defining the effect of basil cultivar (‘Kasia’ and ‘Wala’), as well as of the differentiated doses of CaCO3 (6 or 12 g·dm-3 of substratum) upon the biological value and chemical composition of the oil in the examined plants, grown in pots filled with transitory peat. A significant interaction was reported between the examined cultivar and CaCO3 dose upon the yield of fresh basil plant weight. The highest yield (172.3 g·plant-1) was obtained from plants of ‛Kasia’ cultivar, when a higher dose of CaCO3 was applied (12 g·dm-3 substratum). Lack of significant effect of calcium carbonate dose was reported upon the yield of fresh basil plant weight. In the examined objects similar fresh weight yield values were obtained. At a higher dose of calcium carbonate the unit plant weight equaled 165.9 g·plant-1, and at a lower dose of CaCO3 it was 160.2 g·plant-1. The percentage of dry matter significantly depended on the cultivar. Basil of ‘Wala’ cultivar had higher dry matter content, (32.0%) compared to ‘Kasia’ (25.6%). Both the cultivar and calcium carbonate dose significantly affected the vitamin C contents in fresh basil herb. Increasing the CaCO3 dose from 6 g·dm-3 to 12 g·dm-3 caused the increase of vitamin C concentration by 12.6%. Higher essential oil contents was characteristic of the herb of ‘Kasia’ plants – 1.33% compared to ‘Wala’ cultivar, in whose herb 1.03% of oil was determined. The qualitative composition of isolated oil depended on the examined factors. The predominating compounds were: linalool, 1.8-cineole, geranyl, D germacrene, γ-cadinene, Epi-α -cadinole. A certain differentiation was found in the contents of particular compounds in the essential oils of ‘Kasia’ and ‘Wala’ plants, as the effect of calcium nutrition.
Basil, cultivated as a medicinal and seasoning plant, has strict requirements as to climate, as well as soil fecundity and humidity. The herb harvested during flowering period, contains, besides valuable essential oil, plant dye, vitamins and mineral components. The undertaken studies were aimed at analyzing the contents of macro and microelements in the herbs of two basil cultivars (‘Kasia’ and ‘Wala’), depending on the doses of calcium carbonate – 6 and 12 g·dm-3 substratum. The total nitrogen contents was on quite an even level – from 5.23 to 5.43% d.m. A significant effect of the examined cultivar was reported upon the N-total contents, whereas the calcium carbonate dose did not differentiate the content of this component in a plant. Significant effects of calcium fertilizer dose and cultivar were reported upon potassium and calcium contents in basil herb. The double dose of CaCO3 caused the decrease of potassium and calcium amounts in the plant. Higher concentrations of K and Ca were found in the herb of ‘Kasia’ than ‘Wala’ cultivar. Higher zinc, manganese and copper contents were found in plants of ‘Wala’ cultivar, as compared to plants of ‘Kasia’ cultivar. Doubling the dose of calcium carbonate caused the decrease of manganese, copper and iron concentrations in basil herb, as compared to a single dose. Only as to zinc no such response was reported. The concentrations of nutrients in the examined substratum was differentiated depending on the analyzed factors.
The influence of nitrogen rate and type of potassium fertilizer (KCl, K2S04, KC1 + K2S04) on yield, essential oil content and mineral composition of thyme herb was examined in the greenhouse experiment. Analyzed factors did not significantly affect the plant's yielding. No negative effects of chloride ions on plant's yielding and EC value in medium were ob­served. Total content of essential oil in thyme herb ranged from 3.09% to 4.13%. When us­ing KCl, the increase of oils in herb occurred along with the increase of nitrogen rate. The inverse dependence was recorded when K2S04 was applied. Increasing nitrogen rate and various potassium fertilizers influenced on nutrients contents in thyme herb. The plant showed elevated tendency to accumulate the minerals (N-tot, N-N03, Ca, Mg, S-S04, CI) in herb due to increasing nitrogen fertilization.
The experiments carried out in 2006–2007 referred to the growth of marjoram plants, its yields and chemical composition depending on the plant density and harvest time. Four plant densities (setting spacing 20 × 40 cm, 30 × 30 cm, 30 × 40 cm and 40 × 40 cm) as well as two harvest times (beginning of flower bud forming and full blooming) were applied. The lowest density favored the plant tillering, which apparently affected the herb yield. Plants growing at the lowest density (40 × 40 cm spacing) had the largest fresh and air-dried herb yields (0.71 and 0.18 kg m⁻², respectively). Herb of plants grown at denser spacing contained more total nitrogen and nitrates (V) as well as less potassium, than that of plants grown at lower density. The herb harvest at full flowering stage turned out to be favourable because of significantly lover nitrates concentration as compared to other stages. The contents of essential oil was significantly higher in the full flowering stage than in those harvested in the bud formation stage. Plants growing at denser spacing produced more oil. Trans-sabinene hydrate and terpinene-4-ol were dominating components of marjoram essential oil.
Garden savory (Satureja hortensis L.) is an aromatic spice plant commonly used in the food and pharmaceutical industries. Biological value of this plant is influenced by biotic and abiotic conditions, which include fertilization and date of harvesting. The research was aimed at determining the effect of differentiated nitrogen fertilization (nitrogen doses 0, 4, 8, 12, 16 g·m–2) and different harvesting time (initial flowering and full flowering phase) on yield of fresh plant weight, yield of essential oils, dry matter, protein, L-ascorbic acid contents in the raw material of garden savory of Saturn cv. The highest yield of fresh mass and essential oil was recorded in the herb harvested in the initial flowering phase after applying the highest doses of nitrogen. The obtained results allow to conclude that the greatest influence on the content of L-ascorbic acid in the herb had a dose of 4 g N∙m–2 in both examined dates. The highest amount of protein and dry matter was found in savory raw material after applying the highest dose of nitrogen in the full flowering phase.
The examinations were aimed at determining the dependence between form and rate of applied nitrogen fertilizer vs. yield and chemical composition of leaves of Swiss chard cultivated in greenhouse in spring. Nitrogen was applied in a form of urea, potassium nitrate, and ammonium nitrate at three amounts: 0.2; 0.4; 0.6 g N·dm⁻³ of substrate. Following items were assessed: yield, nutrients contents, as well as chemical analyses of substrate after plant harvest were made. Increase of nitrogen rate in objects with potassium nitrate and ammonium nitrate resulted in the decrease of fresh matter yield, while in objects with urea, the yield remained at constant level. The highest yield of above ground parts (356 g·plant⁻¹) was achieved by fertilizing the plants with the lowest nitrogen rate (as ammonium nitrate). Contents of nitrates in leaf dry matter was within the range of 0.59–1.27% depending on nitrogen rate and form. The highest nitrate levels were found when potassium nitrate was applied as fertilizer, whereas the lowest – when applying ammonium nitrate; however, regardless the fertilizer type, higher rates caused the increase of nitrate contents. Studies revealed that at increasing nitrogen concentration in a substrate, level of vitamin C also increased. Contents of N, P, K, Ca, and Mg in Swiss chard’s leaves depended on nitrogen fertilizer type. Comparison of studied factors influence on potassium concentration in plants indicated that increasing nitrogen rates in objects with urea and ammonium nitrate was accompanied by the decrease of this element content. An inverse dependence was recorded in objects with potassium nitrate, where content of potassium in Swiss chard’s leaves increased along with the nitrogen level increase. Application of 0.2 g N· dm⁻³ substrate appeared to be the most profitable in spring cultivation of Swiss chard, because the largest yields of fresh matter and the lowest share of nitrates in dry matter of studied plant were found.
Fertilizing cultivable plants is an effective way of improving yield quantity and quality. The studies conducted in the years 2008–2009 were aimed at determining the relationship between the kind of nitrogen fertilizer, manner of its application, as well as the dose of calcium carbonate and the plant unit weight, as well as chemical composition of spinach beet, grown in glasshouse in the spring period. Nitrogen was applied in the form of ammonium sulphate and urea in a localized form (N-deposit) and in the form of solution. Calcium was administered in the form of calcium carbonate in the doses of 5 and 15 g · dm-3 of substratum. The length of leaves, plant unit weight and selected parameters of beet leaf utility value were assessed. The substratum was also analyzed after plant harvest. The highest unit weight of plants was obtained with the application of ammonium sulfate in the form of a solution with a lower calcium carbonate dose, compared to the remaining combinations. The examined spinach beet plants were distinguished by high contents of dry matter, protein, total nitrogen, potassium, calcium and magnesium The chemical composition of leaves was significantly differentiated, depending on the examined factors. The contents of nitrates in the leaf dry matter ranged from 0.26 to 0.45%, depending on the kind of nitrogen fertilizer and manner of its application. The applied nitrogen fertilization to a small extent influenced the concentration of nitrates in beet leaves. More nitrates were contained in plants fed by a higher dose of calcium carbonate, compared to the plants which were given a lower dose of CaCO3. The highest content of vitamin C and the lowest share of nitrates in leaf dry matter were obtained with the application of urea in the form of deposit with a lower dose of calcium carbonate.
In the soilless cultivation of tomato under covers the main factors determining the quantity and quality of obtained yield are the kind of substratum used, as well as the content of nutritional solution dosed under plants. Studies conducted in the glasshouse in the years 2005–2007 were aimed at determining the effect of the substratum of rockwool, perlite and expanded clay, as well as two kinds of nutritreint solution of differentiated macroelement concentrations (EC I – 2.4 mS·cm⁻¹ and EC II – 3.6 mS·cm⁻¹) upon yielding and chemical composition of leaves and fruits of tomato cv. 'Cunero F1', grown in extended cycle (22 clusters). Cultivation was conducted with the use of dripping fertigation system, with closed nutrient solution circuit, without recirculation. In the conducted studies no significant differences were found in the total and marketable yield of tomato grown in the examined substrata. Kind of substratum did not also have any significant effect upon the mean weight of one fruit and number of fruits from a plant. In objects cultivated with a solution with higher macroelement concentration (EC II) significantly higher marketable yield was reported, as well as higher fruit unit weight and less non-marketable fruits compared to the basic nutrient solution (EC I). In the leaves of plants fertilized with solution containing 25% more macroelements (EC II) significantly more total nitrogen, potassium, calcium and magnesium was reported. The fruits of plants fertilized with solution of higher macronutrients concentration (EC II) contained significantly more dry matter (5.71%), nitrogen (2.41% d.m.), phosphorus (0.32% d.m.), potassium (4.23% d.m.), calcium (1096 mg·kg⁻¹ d.w.) and less vitamin C (17.2 mg·100 g⁻¹ fr.w.) compared to fruit from plants fertilized with basic nutrient solution of EC 2.4 mS·cm⁻¹. In the studies no significant effect of substratum type was found upon the contents of vitamin C, sugars, total nitrogen, phosphorus, calcium and magnesium in tomato fruit.
Tarragon is a spice but also used for medicinal purposes. In Poland, tarragon plants are grown on plantations to produce herbage used as raw material. The aim of the study was to evaluate the yield of tarragon grown from seedlings in south-eastern Poland and to assess the content of minerals and essential oils in raw material. The experiment was established using seedlings produced in a greenhouse and planted at 30 x 40 cm spacing. The essential oil content in ground herbs was determined in a Deryng apparatus according to a method defined in Pharmacopoeia. Qualitative and quantitative composition of essential oils was characterized by means of gas chromatography coupled with the mass spectrometry (GC/MS) technique. Ground herb underwent determinations of total nitrogen, ammonia, nitrates, phosphorus, potassium, calcium, magnesium and sulfur. The level of atmospheric precipitation considerably affected the height of tarragon plants and fresh herb yields. Total nitrogen and potassium were dominant elements in tarragon herb (32.0 g kg–1 d.m. and 28.8 g kg–1 d.m., respectively). The content of essential oils in herb ranged from 0.75 to 0.95%. Presence of 35 compounds, including two unidentifiable ones, was detected in the essential oil. Most of the identified compounds were monoterpenes. The following substances dominated in the essential oil: elemicin (56.0%), sabinene (20.9%), methyleugenol (6.2%), and E-asarone (6.2%). These compounds belong to different chemical classes.
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