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Changing morphology in the human cotyledon subjected to dual perfusion in vitro exposed to variable magnetic fields

100%

Lopucki M., Lancut M., Kankofer M., Rogowska W., Czerny K., Jedrych B., Pietruszewski S., Stachowicz N., Kornarzynski K., Kotarski J.

Polish Journal of Environmental Studies

2004

tom 13

nr Suppl.2, Part 1

The paper presents the results of morphological examinations of the human cotyledons perfused in vitro and exposed to variable magnetic field (MF) during the 180-minute experiment. The cotyledon biopsies were collected immediately after perfusion and morphologically examined using the electron microscope. The control group C (10 perfusions) was not exposed to MF. In the experimental group E, (10 perfusions), the cotyledons were exposed to the 2mT, 50Hz variable magnetic field while in the experimental group E2 (10 perfusions) the 5mT, 50 Hz was used. In the groups E, and E2, numerous indentations of the sheath (areola) in the syntrophoblast nuclei were found, condensed (thickened) nuclear chromatin right beneath the sheath was substantially reduced and pyknosis was observed in some nuclei. The villi revealed widened vascular - epithelial membrane resulting from the oedema of the endothelial cells. Moreover, an increased number of collagen fibres in the villi and decreased number of active mitochondria were observed in the group E2

The influence of the magnetic field on the glucose consumption and lactic acid production in the in vitro perfused human placental cotyledon

100%

Lopucki M., Kankofer M., Pietruszewski S., Kornarzynski K., Rogowska W., Stachowicz N., Semczuk-Sikora A., Czekierdowski A., Kotarski J.

Polish Journal of Environmental Studies

2004

tom 13

nr Suppl.2, Part 1

Objective: The biological effects of variable magnetic fields (MF) generated by widely used electrical devices on living organisms are not well understood. However MF may be potentially hazardous for pregnant women, this problem was not profoundly studied. The aim of this study was to evaluate the influence of the variable magnetic field (MF) on the placental metabolism by measurement of glucose consumption and lactate production in the human placenta under dual recirculating perfusion conditions. Material and methods: Altogether 20 term human placental cotyledons were exposed to homogeneous variable MF of B = 2 mT and f = 50 Hz (n=10; group E,) or of B = 5 mT and f = 50 Hz (n=10; group E2) during 3 hours dual close perfusion in vitro. 10 term placental cotyledons were perfused without an exposure to the MF and they served as a control group. Results: The results showed no effect of used MF on the glucose consumption either in group E, or in group E2in. There was also no influence of MF on lactic acid production in group Er In group E2, however, a significant increase of lactic acid production in the fetal circulation from 90lh to 180th minute and in the maternal circulation at 180 minute was noted. Conclusions: An increase in the lactic acid production in the group E2in observed during the experiment may result from hindered oxygen supply to the placenta and intensified anaerobic metabolism of glucose.

Ograniczanie wyników

2 Polish Journal of Environmental Studies

2 Kankofer M.

2 Kornarzynski K.

2 Kotarski J.

2 Lopucki M.

2 Pietruszewski S.

2 Rogowska W.

2 Stachowicz N.

1 Czekierdowski A.

1 Czerny K.

1 Jedrych B.

1 Lancut M.

1 Semczuk-Sikora A.

2 2004

Wyniki wyszukiwania

Changing morphology in the human cotyledon subjected to dual perfusion in vitro exposed to variable magnetic fields

The influence of the magnetic field on the glucose consumption and lactic acid production in the in vitro perfused human placental cotyledon