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Detection of Babesia bigemina antibodies by Dot-ELISA in cattle and buffaloes

100%

Mishra A. K., Reddy G. G. B., Rao J. R., Tewari A. K.

Acta Parasitologica

1998

tom 43

nr 1

An affinity purified Babesia bigemina piroplasm antigen was successfully employed in a Dot-ELISA for detection of antibodies in buffaloes and catle. A low degree of cross-reactivity was seen against putative B. bigemina (Mexican) and B. bovis undiluted reference serum and no cross-reactivity was observed in seru dilutions of 1:10 and above. Theileria annulata did not show any reactivity with B. bigemina. The assay detected antibodies at 7, 14 or 21 days post-experimental infection in bovine calves with 37.5, 87.5 and 100% seroreactivity, respectively. The incidence of B. bigemina infections was found highest in cattle (19.91) as against 7.4% in buffaloes out of 486 sera samples, examined from Boophilus microplus infested areas from Orissa, Rajasthan and Uttar Pradesh States.

Ograniczanie wyników

1 Acta Parasitologica

1 Mishra A. K.

1 Rao J. R.

1 Reddy G. G. B.

1 Tewari A. K.

1 1998

Wyniki wyszukiwania

Detection of Babesia bigemina antibodies by Dot-ELISA in cattle and buffaloes