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1

Faryngotomia i faryngostomia w zabiegach stomatologicznych i chirurgii szczękowej

100%
Gawor J., Gawor M.
Magazyn Weterynaryjny
|
2014
|
tom 23
|
nr 01
2

Kij w mrowisko, ...czyli propozycja nowej strategii odstrzalu jeleni

81%
Bobek B., Ciepluch Z., Gawor M., Widera E.
Łowiec Polski
|
2000
|
nr 11
12-15
3

The role of drebrin in the neuromuscular junction

81%
Alvarez-Suarez P., Gawor M., Proszynski T. J.
Acta Neurobiologiae Experimentalis
|
2019
|
tom 79
|
nr Suppl.1
INTRODUCTION: Drebrin is an actin-binding protein that regulates cytoskeleton dynamics in different cell types. It plays an important role in shaping dendritic spines and facilitating the stabilization of neurotransmitter receptors at the postsynaptic membrane in the CNS. Its role at the neuromuscular junction (NMJ) in the PNS has not been investigated. AIM(S): In the present study we aimed to explore the role of Drebrin in this special type of synapse and to study its mechanism of action. METHOD(S): We used an in vitro model of C2C12‑derived myotubes in which Drebrin1 expression is silenced with siRNA or its actin-binding function is blocked by a BTP2 inhibitor. To address the role of Drebrin at the postsynaptic machinery, we used both biochemical and immunohistochemical approaches. RESULTS: We found that Drebrin colocalizes with acetylcholine receptors (AChR) at the surface of myofibers in vivo and in vitro, and its depletion causes impairments in receptor aggregation and clusters complexity, suggesting a crucial role in the regulation of these processes. We assessed whether drebrin inhibition affects the expression levels and cell surface delivery of AChRs or the microtubule organization underneath AChRs. Our experiments revealed that drebrin depletion in cultured myotubes affects the organization of cortical microtubules, which has been previously shown to be indispensable for incorporation of newly synthesized AChR into the postsynaptic specialization. CONCLUSIONS: We found that Drebrin is a component of the muscle postsynaptic machinery and it plays an important role in their organization. The mechanism through which Drebrin regulates AChR clustering appears to occur through its interaction with EB3, that leads to the recruitment of microtubules and allows the stabilization of AChRs. FINANCIAL SUPPORT: This research was supported by the National Science Centre grants: UMO‑2018/29/B/ NZ3/02675, UMO‑2016/21/D/NZ4/03069, and UMO- ‑2018/29/N/NZ3/02682.
4

Role of Amot12, Rast8 and Homer1 in the organization of postsynaptic machinery

61%
Gawor M., Niewiadomski P., Bernadzki K., Jozwiak J., Rojek K., Redowicz M. J., Proswzynski T. J.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: Vertebrate neuromuscular junction (NMJ) undergoes a series of topological rearrangements in order to achieve its mature complex shape resembling pretzels. We have previously reported that podosomes, actin-rich dynamic adhesive organelles are implicated in the NMJ developmental remodeling. The main aim of this study was to understand molecular mechanisms regulating formation of podosomes and/or remodeling of the postsynaptic machinery with a special focus on the role of Amotl2 scaffold protein in these processes. METHODS: To identify Amotl2-binding proteins we used TAP-tag affinity purification and mass spectrometry. Localization of proteins to NMJ subsynaptic compartments was performed using standard cytochemical procedures and confocal microscopy. We performed RNAi-based knockdown experiments on cultured C21C12 myotubes to assess the importance of proteins in the organization of postsynaptic AChR clusters. RESULTS: We identified several novel Amotl2-binding proteins and subsequently focused our experiments on two of them, Rassf8 and Homer1, that remain uncharacterized at the NMJ. Amotl2, Rassf8 and Homer1 are concentrated at postsynaptic areas of NMJs in the indentations between the AChR-rich branches. Our results suggest that Rassf8 and Homer1 may be involved in AChR organization and development of the neuromuscular synapses. CONCLUSIONS: We identified novel components of the muscle postsynaptic machinery that specifically localize to the sites of NMJ remodeling. Our results suggest that Amotl2 may be involved in the developmental remodeling of the postsynaptic machinery through the interactions with Rassf8 and Homer1. This research was supported by the NCN grant 2012/05/E/ NZ3/00487.
5

Liprin-alpha-1 is a novel component of the neuromuscular junction and involved in the organization of the postysynaptic machinery

61%
Bernadzki K. M., Pezinski M., Gawor M., Mazurek P., Niewiadomski P., Redowicz J., Proszynski T. J.
Acta Neurobiologiae Experimentalis
|
2017
|
tom 77
|
nr Suppl.1
INTRODUCTION: Neuromuscular junctions (NMJs) are specialized synapses formed between the motor neurons and skeletal muscles. The proper functioning of the NMJ is important for activities involving muscle contraction including breathing. Liprin‑α‑1 was previously identified in a biochemical screen as an interactor of α‑dystrobrevin‑1, a component of dystrophin-associated glycoprotein complex (DGC) responsible for the attachment of the postsynaptic machinery to the extracellular matrix. Liprin‑α‑1 has been shown to be an organizer of synapses in the central nervous system and our study presents an insight into the localization and function of this protein at the vertebrate NMJ. AIM(S): To unravel the localization and function of liprin‑α‑1 at the vertebrate NMJ. METHOD(S): We employed immunohistochemical, siRNA-mediated RNAi techniques and confocal microscopy techniques. RESULTS: We show that liprin‑α‑1 localizes to the postsynaptic site of NMJ in a nerve-dependent manner, and its localization is maintained throughout postnatal development. Liprin‑α‑1 plays a crucial role in the formation of the postsynaptic machinery since liprin‑α‑1 depleted myotubes failed to cluster postsynaptic acetylcholine receptors (AChRs). We provide evidence that liprin‑α‑1 plays a role in the attachment of microtubule ends to the cell cortex, where they stabilize the postsynaptic machinery and promote clustering of AChRs. Consistently with this observation abrupt disruption of microtubules with in cultured myotubes did not affected preexisting AChR clusters, but abolished formation of the new assemblies. CONCLUSIONS: Our results show that Liprin‑α‑1 is important component of the postsynaptic machinery involved in AChR clustering. FINANCIAL SUPPORT: This research was supported by the NCN grants 2012/05/E/NZ3/00487, 2015/19/N/ NZ5/02268, 2014/13/B/NZ3/00909, and 2013/09/B/ NZ3/03524.
6

Novel Alpha-Dystrobrevin interactors regulate neuromuscular junction postsynaptic machinery

61%
Gawor M., Gingras J., Bernadzki K., Niewiadomski P., Sanes R., Proszynski T. J.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: Alpha-Dystrobrevin-1 (aDB1) is a component of the dystrophin-associated glycoprotein complex (DGC), which stabilizes the neuromuscular junction (NMJ) postsynaptic machinery. The goal of our research is to gain insight into the mechanism of aDB-1 function at the NMJ, with special focus on the role of aDB1 phosphorylation, which was previously shown to be critical for proper synaptic organization. METHODS: To determine the importance of each tyrosine phosphorylation site, we expressed mutant proteins lacking individual sites in myotubes. Next, we developed phospho-specific antibodies and used them to analyze the level of aDB1 phosphorylation during NMJ remodeling. We also performed a biochemical screen to identify general and phospho-specific aDB1-binding proteins. Finally, we performed RNAi experiments on cultured myotubes to demonstrate the importance of these proteins in the organization of AChR clusters. RESULTS: Our experiments identified Liprin-α1, α-Catulin and Usp9x as novel aDB1-interacting proteins. We have demonstrated that aDB1 phosphorylation is dynamically regulated during NMJ remodeling in development and upon denervation and that the phosphorylation at the most critical tyrosine Y713 triggers recruitment of aDB1 phospho-specific interacting proteins, including Grb2, SH3BP2, Arhgef5 and PI3K. Subsequently, we demonstrated that Liprin-α1, α-Catulin and Grb2 are associated with the postsynaptic NMJ machinery and are indispensable for proper AChR organization. CONCLUSIONS: Our research highlights the importance of aDB1 phosphorylation in the remodeling of neuromuscular synapses. We identify several novel aDB1-interacting components of the postsynaptic machinery, which play important roles in its organization. This research was supported by the grant 2013/09/B/NZ3/03524 from the National Science Centre (NCN).
7

Niski poziom czynnika VII jest czynnikiem predykcyjnym występowania ciężkich krwawień wewnątrzczaszkowych u noworodków z ekstremalnie niską masą ciała

51%
Dabrowska-Wojciak I., Mikinka M., Gawor M., Januszewska E., Walas W., Stepnicka-Bindemann M., Sobala W., Piotrowski A.
Diagnostyka Laboratoryjna
|
2007
|
tom 43
|
nr 3
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