Our paper presents biological Cr(VI) removal from water solution by applying a clean fungi culture, namely Aspergillus niger. The growth of the organism and removal of chromium(VI) were done in water solution of various chromium(VI) contents and at optimal pH value. During 14 days of incubation, samples of 5 ml each were collected daily to determinate chromium(VI) contents in solution. Then the efficiency of this biological removal was also specified. The Cr(VI) removal process may occur via reduction, biosorption, or bioaccumulation pathways. To determine which pathway may be used in the study the Cr(III) contents were also determined in the samples as well chromium contents in ooze after mycelium irrigating and in mycelium.