Physiological parameters (vigour and viability, total protein, albumin and globulin contents) were studied in yellow lupin seeds stored for 20 years at -14ºC, 0ºC and at room temperature (approx. 20ºC). The storage of yellow lupin cv. Iryd seeds, at room temperature resulted in viability decrease to 2% and increased seed leachate. No evident differences could be observed in vigour and viability of seeds stored at lowered (0ºC and -14 ºC) temperatures. Protein content was the lowest in seeds stored at +20 ºC and the highest in seeds stored at -14ºC.